Self-Assembled Hydroxyapatite-Graphene Scaffold for Photothermal Cancer Therapy and Bone Regeneration

Li, Dejian; Nie, Wei; Chen, Liang; McCoul, David; Liu, Dinghua; Zhang, Xu; Jin, Ying; Yu, Baoqing; He, Chuanglong

doi:10.1166/jbn.2018.2646

Cited by 73 publications

(45 citation statements)

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“…21,22 On the basis of GO, it is possible for carboxyl, 23 amino, 24 hydroxyl 25 and other functional groups to be introduced not only to improve the fluidity and dispersion of graphene, but also to further endow it with new functions, among which is that various active substances can be loaded to improve bioactivities. 26,27 At present, the functionalized GDs have been compounded with various substrates such as metals, 28 inorganic, 29 natural polymer 30 and synthetic polymer 31 to prepare scaffolds, 32,33 coatings, 34 membranes 35 and injectable hydrogels. 36 Lots of studies in vitro and in vivo have demonstrated that the GDscontaining composites could regulate the extracellular microenvironment to effectively promote bone regeneration.…”

Section: Introductionmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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During continuous innovation in the preparation, characterization and application of various bone repair materials for several decades, nanomaterials have exhibited many unique advantages. As a kind of representative two-dimensional nanomaterials, graphene and its derivatives (GDs) such as graphene oxide and reduced graphene oxide have shown promising potential for the application in bone repair based on their excellent mechanical properties, electrical conductivity, large specific surface area (SSA) and atomic structure stability. Herein, we reviewed the updated application of them in bone repair in order to present, as comprehensively, as possible, their specific advantages, challenges and current solutions. Firstly, how their advantages have been utilized in bone repair materials with improved bone formation ability was discussed. Especially, the effects of further functionalization or modification were emphasized. Then, the signaling pathways involved in GDs-induced osteogenic differentiation of stem cells and immunomodulatory mechanism of GDs-induced bone regeneration were discussed. On the other hand, their applications as contrast agents in the field of bone repair were summarized. In addition, we also reviewed the progress and related principles of the effects of GDs parameters on cytotoxicity and residues. At last, the future research was prospected.

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Section: Introductionmentioning

confidence: 99%

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Du¹,

Wang

Zhang³

et al. 2020

IJN

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“…The near-infrared (NIR) laser irradiation significantly elevates the local temperatures around photothermal agents to kill cancer cells via hyperthermia, and inhibit the expression of the metastasis-related factors such as matrix metalloproteinase, twist and transforming growth factor-β1 [24]. Notably, graphene oxide (GO) with excellent light-absorbing property and photothermal conversion efficiency has been widely used for PTT application [20,25]. Moreover, the carbonyl, epoxy and hydroxyl groups in the GO can serve as bioactive sites for cell proliferation and bone mineralization via activating MAPK signaling pathway [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Graphene-modified CePO4 nanorods effectively treat breast cancer-induced bone metastases and regulate macrophage polarization to improve osteo-inductive ability

Liu

et al. 2021

J Nanobiotechnol

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Background Breast cancer bone metastasis has become one of the most common complications; however, it may cause cancer recurrence and bone nonunion, as well as local bone defects. Methods Herein, In vitro, we verified the effect of bioscaffold materials on cell proliferation and apoptosis through a CCK8 trial, staining of live/dead cells, and flow cytometry. We used immunofluorescence technology and flow cytometry to verify whether bioscaffold materials regulate macrophage polarization, and we used ALP staining, alizarin red staining and PCR to verify whether bioscaffold material promotes bone regeneration. In vivo, we once again studied the effect of bioscaffold materials on tumors by measuring tumor volume in mice, Tunel staining, and caspase-3 immunofluorescence. We also constructed a mouse skull ultimate defect model to verify the effect on bone regeneration. Results Graphene oxide (GO) nanoparticles, hydrated CePO4 nanorods and bioactive chitosan (CS) are combined to form a bioactive multifunctional CePO4/CS/GO scaffold, with characteristics such as photothermal therapy to kill tumors, macrophage polarization to promote blood vessel formation, and induction of bone formation. CePO4/CS/GO scaffold activates the caspase-3 proteasein local tumor cells, thereby lysing the DNA between nucleosomes and causing apoptosis. On the one hand, the as-released Ce3+ ions promote M2 polarization of macrophages, which secretes vascular endothelial growth factor (VEGF) and Arginase-1 (Arg-1), which promotes angiogenesis. On the other hand, the as-released Ce3+ ions also activated the BMP-2/Smad signaling pathway which facilitated bone tissue regeneration. Conclusion The multifunctional CePO4/CS/GO scaffolds may become a promising platform for therapy of breast cancer bone metastases.

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“…This suggests that the presence of GO and HA was responsible for the slight suppression of the proliferation of the rBMSCs that were seeded on the test materials. This claim is supported by several preceding studies which reported that the presence of numerous surface functional groups in GO increases intracellular reactive oxygen species levels which may somehow induce cell apoptosis [29][30][31]. HA has also been considered to demonstrate some levels of cytotoxicity mainly due to its intrinsic characteristics like alkalinity [14,32].…”

Section: In Vitro Analysismentioning

confidence: 60%

Preparation and characterizations of three-dimensional porous collagen/graphene oxide/hydroxyapatite nanocomposite scaffolds for bone tissue engineering

Zimba

Jiang²,

Li³

et al. 2019

OSJ

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Studies have reported that the incorporation of graphene oxide (GO) and hydroxyapatite (HA) into biocompatible polymers (such as collagen (Col), chitosan, alginate, etc) results in enhanced structural and mechanical properties respectively. The objective of this study was to prepare and characterize three-dimensional (3D) porous Col/GO/HA nanocomposite scaffolds and to investigate cytocompatibility and osteogenic differentiation potential of rat bone marrow mesenchymal stem cells (rBMSCs) on the as-prepared scaffolds. The SEM images revealed that the scaffolds were porous with the pore diameter inversely proportional to the concentration of HA. XRD results were able to depict the characteristic peaks for HA which shows that HA was incorporated into the scaffolds. The rBMSCs which were cultured on the scaffolds were able to attach and proliferate during the 21 days of the experiment which indicates that the as-prepared scaffolds are cytocompatible. The Alizarin red staining demonstrated the presence of calcium deposits as there were orange-red stains on the samples after culturing the cells using the osteogenic differentiation medium. These results demonstrate the promising potential of the 3D porous Col/GO/HA nanocomposite scaffolds for applications in bone tissue engineering.

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Self-Assembled Hydroxyapatite-Graphene Scaffold for Photothermal Cancer Therapy and Bone Regeneration

Cited by 73 publications

References 0 publications

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

<p>Applications of Graphene and Its Derivatives in Bone Repair: Advantages for Promoting Bone Formation and Providing Real-Time Detection, Challenges and Future Prospects</p>

Graphene-modified CePO4 nanorods effectively treat breast cancer-induced bone metastases and regulate macrophage polarization to improve osteo-inductive ability

Preparation and characterizations of three-dimensional porous collagen/graphene oxide/hydroxyapatite nanocomposite scaffolds for bone tissue engineering

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