2022
DOI: 10.3390/cryst12070976
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Abstract: Heavy-atom soaking has been a major method for experimental phasing, but it has been difficult for membrane proteins, partly owing to the lack of available sites in the scarce soluble domain for non-invasive heavy-metal binding. The lipid cubic phase (LCP) has proven to be a successful method for membrane protein crystallization, but experimental phasing with LCP-grown crystals remains difficult, and so far, only 68 such structures were phased experimentally. Here, the selenourea was tested as a soaking reagen… Show more

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“…51,52 Selenium is also introduced into crystals of proteins and nucleic acids by soaking with SeU and can be a convenient vehicle for solving phase correction problems. 54,55 Fig. 1E shows the binding sites of SeU in a cyan-uorescent protein where the tryptophan-based chromophore serves as the intrinsic uorophore.…”
Section: Introductionmentioning
confidence: 99%
“…51,52 Selenium is also introduced into crystals of proteins and nucleic acids by soaking with SeU and can be a convenient vehicle for solving phase correction problems. 54,55 Fig. 1E shows the binding sites of SeU in a cyan-uorescent protein where the tryptophan-based chromophore serves as the intrinsic uorophore.…”
Section: Introductionmentioning
confidence: 99%