Selective isolation of magnetic nanoparticle-mediated heterogeneity subpopulation of circulating tumor cells using magnetic gradient based microfluidic system

Kwak, Bongseop; Lee, Jun Ho; Lee, Dongkyu; Lee, Kang-Ho; Kwon, Ohwon; Kang, Sang Mo; Kim, Youngwoo

doi:10.1016/j.bios.2016.08.002

Cited by 58 publications

(37 citation statements)

References 17 publications

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“…The systems used Fe 3 O 4 NPs functionalized with (3‐glycidylox‐ypropyl)tri‐methoxysilane for immobilization of EpCAm antibody on their surface for the specific binding with CTCs. The system was able to isolate 3 mL of the heterogeneous CTC sample in 1 h with high isolating yield demonstrating its potential to shorten the date to diagnose the cancer metastasis in clinic . A different approach through a microfluidic platform comprising three different modules demonstrated high‐throughput separation of cancer cells from blood and on‐chip organization of those cells for streamlined analysis.…”

Section: Representative Biomedical Applicationsmentioning

confidence: 99%

Advances in Magnetic Nanoparticles for Biomedical Applications

Cardoso

Francesko

Ribeiro

et al. 2017

Adv Healthcare Materials

525

330

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Magnetic nanoparticles (NPs) are emerging as an important class of biomedical functional nanomaterials in areas such as hyperthermia, drug release, tissue engineering, theranostic, and lab-on-a-chip, due to their exclusive chemical and physical properties. Although some works can be found reviewing the main application of magnetic NPs in the area of biomedical engineering, recent and intense progress on magnetic nanoparticle research, from synthesis to surface functionalization strategies, demands for a work that includes, summarizes, and debates current directions and ongoing advancements in this research field. Thus, the present work addresses the structure, synthesis, properties, and the incorporation of magnetic NPs in nanocomposites, highlighting the most relevant effects of the synthesis on the magnetic and structural properties of the magnetic NPs and how these effects limit their utilization in the biomedical area. Furthermore, this review next focuses on the application of magnetic NPs on the biomedical field. Finally, a discussion of the main challenges and an outlook of the future developments in the use of magnetic NPs for advanced biomedical applications are critically provided.

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Section: Representative Biomedical Applicationsmentioning

confidence: 99%

Advances in Magnetic Nanoparticles for Biomedical Applications

Cardoso

Francesko

Ribeiro

et al. 2017

Adv Healthcare Materials

525

330

View full text Add to dashboard Cite

show abstract

“…Copyright (2016) American chemical society. (C) Magnetic force gradient based microfluidic chip [46]; reproduced by permission of Elsevier. (D) Immunofluorescence isolation method based on microfluidics and line confocal microscopy.…”

Section: Label‐dependent Technologiesmentioning

confidence: 99%

“…Figure 1C shows a magnetic force gradient‐based microfluidic chip that can separate CTCs depending on their expression level of EpCAM. This device can isolate CTCs from 3 mL blood within 1 h [46]. Among negative isolation methods, magnetic nanobeads coated with anti‐CD45 antibodies have been commonly used for separating WBCs from blood, thereby enriching CTCs [47].…”

Section: Label‐dependent Technologiesmentioning

confidence: 99%

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasis

Jiang

Wang

et al. 2020

Electrophoresis

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Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasisThe prognosis of malignant tumors is challenged by insufficient means to effectively detect tumors at early stage. Liquid biopsy using circulating tumor cells (CTCs) as biomarkers demonstrates a promising solution to tackle the challenge, because CTCs play a critical role in cancer metastatic process via intravasation, circulation, extravasation, and formation of secondary tumor. However, the effectiveness of the solution is compromised by rarity, heterogeneity, and vulnerability associated with CTCs. Among a plethora of novel approaches for CTC isolation and enrichment, microfluidics leads to isolation and detection of CTCs in a cost-effective and operation-friendly way. Development of microfluidics also makes it feasible to model the cancer metastasis in vitro using a microfluidic system to mimick the in vivo microenvironment, thereby enabling analysis and monitor of tumor metastasis. This paper aims to review the latest advances for exploring the dual-roles microfluidics has played in early cancer diagnosis via CTC isolation and investigating the role of CTCs in cancer metastasis; the merits and drawbacks for dominating microfluidics-based CTC isolation methods are discussed; biomimicking cancer metastasis using microfluidics are presented with example applications on modelling of tumor microenvironment, tumor cell dissemination, tumor migration, and tumor angiogenesis. The future perspectives and challenges are discussed.

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“…These models are highly useful for studying cell–cell interactions and the influence of the tumor microenvironment. They also have unique applications in pathological analysis, including the screening of tumor stem cells and circulating tumor cells (Kwak et al, ). There are already a number of heterogeneous tumor models used for drug development and screening.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

Review on biofabrication and applications of heterogeneous tumor models

Liu

Yao

Pang

et al. 2019

J Tissue Eng Regen Med

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Resolving the origin and development of tumor heterogeneity has proven to be a crucial challenge in cancer research. In vitro tumor models have been widely used for both scientific and clinical research. Currently, tumor models based on 2D cell culture, animal models, and 3D cell‐laden constructs are widely used. Heterogeneous tumor models, which consist of more than one cell type and mimic cell–cell as well as cell–matrix interactions, are attracting increasing attention. Heterogeneous tumor models can serve as pathological models to study the microenvironment and tumor development such as tumorigenesis, invasiveness, and malignancy. They also provide disease models for drug screening and personalized therapy. In this review, the current techniques, models, and oncological applications regarding 3D heterogeneous tumor models are summarized and discussed.

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Selective isolation of magnetic nanoparticle-mediated heterogeneity subpopulation of circulating tumor cells using magnetic gradient based microfluidic system

Cited by 58 publications

References 17 publications

Advances in Magnetic Nanoparticles for Biomedical Applications

Advances in Magnetic Nanoparticles for Biomedical Applications

Microfluidic applications on circulating tumor cell isolation and biomimicking of cancer metastasis

Review on biofabrication and applications of heterogeneous tumor models

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