Selective Inhibitors of Cyclooxygenase-2 Delay the Activation of Nuclear Factor κB and Attenuate the Expression of Inflammatory Genes in Murine Macrophages Treated with Lipopolysaccharide

Callejas, Nuria A.; Fernández-Martínez, Amalia; Castrillo, Antonio; Martı́n-Sanz, Paloma

doi:10.1124/mol.63.3.671

Cited by 19 publications

(11 citation statements)

References 41 publications

(45 reference statements)

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“…4 and 5) was carried out. Rofecoxib (10 M) was previously shown to suppress COX2 and iNOS expression levels after 18 h of LPS activation, and the inhibition was LPS-dosedependent (effect was observed only at 100 ng/ml or lower LPS concentrations) (Callejas et al, 2003). In our experimental system optimized with 6 h of LPS activation at 1 g/ml, rofecoxib was unable to significantly inhibit gene expression (Fig.…”

Section: Anti-inflammatory Activity Of Peitc 331mentioning

confidence: 86%

In Vitro and in Vivo Anti-Inflammatory Activity of a Seed Preparation Containing Phenethylisothiocyanate

Dey¹,

Ribnicky²,

Kurmukov³

et al. 2005

J Pharmacol Exp Ther

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Winter cress (Barbarea verna) seed preparations rich in phenethylisothiocyanate (PEITC) had strong in vivo and in vitro anti-inflammatory activity, significantly reducing the size of carrageenan-induced rat paw edema. This in vivo effect was comparable with that of the nonsteroidal anti-inflammatory drug aspirin. The seed preparation, in a concentration-dependent manner, reduced the mRNA levels of inflammation-related genes such as the inducible forms of cyclooxygenase and nitric-oxide synthase and the proinflammatory cytokine interleukin in lipopolysaccharide-stimulated mouse macrophage cell line RAW 264.

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Section: Anti-inflammatory Activity Of Peitc 331mentioning

confidence: 86%

In Vitro and in Vivo Anti-Inflammatory Activity of a Seed Preparation Containing Phenethylisothiocyanate

Dey¹,

Ribnicky²,

Kurmukov³

et al. 2005

J Pharmacol Exp Ther

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“…After incubation for 4 hours, nonadherent cells were removed, and remnant cells were cultured for the indicated times. 16 …”

Section: Preparation Of Macrophagesmentioning

confidence: 99%

Selective Impairment of Nuclear Factor-κB-Dependent Gene Transcription in Adult Cardiomyocytes

Cuenca

Goren

Prieto

et al. 2007

The American Journal of Pathology

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The ability of neonatal and adult cardiomyocytes to activate the nuclear factor (NF)-B pathway in response to lipopolysaccharide and interleukin-1␤ challenge has been investigated and compared with that of peritoneal macrophages. The activation of the IB kinase and the phosphorylation and degradation of IB␣ and IB␤ was much lower in adult cardiomyocytes than in the neonatal counterparts and macrophages. This restricted activation of the NF-B pathway resulted in a significant reduction in the time of nuclear activation of NF-B, as deduced by electrophoretic mobility shift assays and in the transcription of target genes, such as IB␣, cyclooxygenase-2 (COX-2) and nitric-oxide synthase-2 (NOS-2). Studies on chromatin immunoprecipitation showed binding of NF-B proteins to the regulatory B sites identified in the promoters of the IB␣, COX-2, and NOS-2 genes in macrophages and, to a lower extent, in neonatal cardiomyocytes. The binding to these B sites in adult cardiomyocytes was observed only in the IB␣ promoter and was minimal or absent in the COX-2 and NOS-2 promoters, respectively, suggesting a restricted activation of NF-B-regulated genes in these cells. These data indicate that the function of the NF-B pathway in adult cardiomyocytes is limited in time, which results in the expression of a reduced number of genes and provides a functional explanation for the absence of NOS-2 inducibility in these cells under proinflammatory conditions. (Am J Pathol

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“…However, there are indications that there may be some actions of the drug on signal transduction pathways that may be of considerable significance. Thus, the binding of the transcription factor NFKB to DNA was inhibited at high concentrations of 10-100 ~molll whereas the binding of API was markedly increased at 100 ~olll concentrations of the drug [337,338]. These concentrations are relatively high and thus caution should be noted about the interpretation of these effects in relationship to known pharmacologically active concentrations of the drug.…”

Section: Pharmacology and Toxicologymentioning

confidence: 99%

COX-2 Inhibitors

Pairet¹,

Ryn²

2004

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Selective Inhibitors of Cyclooxygenase-2 Delay the Activation of Nuclear Factor κB and Attenuate the Expression of Inflammatory Genes in Murine Macrophages Treated with Lipopolysaccharide

Cited by 19 publications

References 41 publications

In Vitro and in Vivo Anti-Inflammatory Activity of a Seed Preparation Containing Phenethylisothiocyanate

In Vitro and in Vivo Anti-Inflammatory Activity of a Seed Preparation Containing Phenethylisothiocyanate

Selective Impairment of Nuclear Factor-κB-Dependent Gene Transcription in Adult Cardiomyocytes

COX-2 Inhibitors

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