Journal of Chromatography A volume 1218, issue 51, P9250-9259 2011 DOI: 10.1016/j.chroma.2011.10.088 View full text
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Rahul D. Sheth, Christopher J. Morrison, Steven M. Cramer

Abstract: A library of displacer analogues with varying degrees of electrostatic, hydrophobic and hydrogen bonding moieties was evaluated for their ability to enhance the selectivity of multimodal (MM) chromatography under high loading conditions. The library was screened for displacement of model proteins using a robotic liquid handling system and selective batch separations were achieved for proteins that were inseparable with linear gradient chromatography. Trends in protein displacement were identified and displacer…

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