Selective control of parasitic nematodes using bioactivated nematicides

Burns, Andrew R.; Baker, Rachel J.; Kitner, Megan; Knox, Jessica; Cooke, Brittany; Volpatti, Jonathan; Vaidya, Aditya S.; Puumala, Emily; Palmeira, Bruna M.; Redman, Elizabeth; Snider, Jamie; Marwah, Sagar; Chung, Sai W.; MacDonald, Margaret H.; Tiefenbach, Jens; Hu, Chun; Xiao, Qin; Finney, Constance A. M.; Krause, Henry M.; MacParland, Sonya A.; Štagljar, Igor; Gilleard, John S.; Cowen, Leah E.; Meyer, Seth D.; Cutler, Sean R.; Dowling, James J.; Lautens, Mark; Zasada, Inga A.; Roy, Peter J.

doi:10.1038/s41586-023-06105-5

Cited by 17 publications

(34 citation statements)

References 63 publications

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“…Nematodes are an enormous threat to global agriculture and food security, and development of environmentally benign botanical nematicides is urgent need. 33 Plant metabolites are an excellent choice for development of botanical nematicides, due to their safety, sustainability, and effectiveness. 11 E. peplus harbors a wealth of specialized metabolites, and DI3A is the most abundant diterpenoid in E. peplus latex, whose content in the latex could reach approximately 1630 μg/mL.…”

Section: ■ Discussionmentioning

confidence: 99%

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Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1

Mu,

Hua,

et al. 2024

J. Agric. Food Chem.

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The latex of Euphorbia peplus and its major component 20-deoxyingenol-3-angelate (DI3A) displayed significant nematicidal activity against Caenorhabditis elegans and Panagrellus redivivus. DI3A treatment inhibited the growth and development of nematodes and caused significantly negative effects on locomotion behavior, reproduction, and accumulation of reactive oxygen species. Transcriptome analysis indicated that differential expression genes in DI3A-treated C. elegans were mainly associated with the metabolism, growth, and development process, which were further confirmed by RT-qPCR experiments. The expression level of TPA-1 gene encoding a protein kinase C isotype was obviously upregulated by DI3A treatment, and knockdown of TPA-1 by RNAi technology in the nematode could relieve the growth-inhibitory effect of DI3A. Metabolic analysis indicated that DI3A was hardly metabolized by C. elegans, but a glycosylated indole derivative was specifically accumulated likely due to the activation of detoxification. Overall, our findings suggested that DI3A from E. peplus latex exerted a potent nematicidal effect through the gene TPA-1, which provides a potential target for the control of nematodes and also suggests the potential application value of E. peplus latex and DI3A as botanical nematicides.

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Section: ■ Discussionmentioning

confidence: 99%

“…Nematodes are an enormous threat to global agriculture and food security, and development of environmentally benign botanical nematicides is urgent need . Plant metabolites are an excellent choice for development of botanical nematicides, due to their safety, sustainability, and effectiveness …”

Section: Discussionmentioning

confidence: 99%

Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1

Mu,

Hua,

et al. 2024

J. Agric. Food Chem.

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“…Recently, Burns et al (2023), when screening uncharacterized compounds that disrupted the growth of the free‐living nematode Caenorhabditis elegans , discovered a new class of nematicides that contain imidazothiazole, and found that they were highly selective in killing only nematodes (Figure 1A). Experiments using various nematode species and a wide range of non‐nematode species and cell types indicated that the newly discovered nematicides, called selectivins, exhibit even higher selectivity for nematodes than most commercially available formulations tested.…”

Section: Figurementioning

confidence: 99%

“…Recently, Burns et al (2023), when screening uncharacterized compounds that disrupted the growth of the free-living nematode Caenorhabditis elegans, discovered a…”

mentioning

confidence: 99%

“…There have been hardly any safer alternatives developed to replace them, which leaves the farmers with limited options to control nematodes, endangering global food security. Therefore, there is great demand to develop nematode-selective nematicides.Recently, Burns et al (2023), when screening uncharacterized compounds that disrupted the growth of the free-living nematode Caenorhabditis elegans, discovered a…”

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The marksman: Bioactivated nematicides selectively kill plant‐parasitic nematodes

Cao

Ikram

Chen

et al. 2023

JIPB

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Plant‐parasitic nematodes (PPNs) are destructive pathogens that cause great damage to crops and severely limit food production, hindering our ability to meet the growing demand for food worldwide (Topalovic and Vestergård, 2021). The global crop losses caused by PPNs are estimated to be about 8.8%‐14.6% of total crop production and 100‐157 billion USD annually (Singh et al., 2013). Due to their small size and fast propagation speed, PPNs are difficult to see with naked eye, hence difficult to control. Farmers employ different ways to reduce or prevent the damage caused by nematodesThis article is protected by copyright. All rights reserved.

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Volatile organic compounds released from entomopathogenic nematode‐infected insect cadavers for the biocontrol of Meloidogyne incognita

Li,

Wei,

Pei

et al. 2024

Pest Management Science

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BACKGROUNDRoot‐knot nematodes (RKNs), Meloidogyne spp., are one of the most destructive polyphagous plant‐parasitic nematodes. They pose a serious threat to global food security and are difficult to control. Entomopathogenic nematodes (EPNs) show promise in controlling RKNs. However, it remains unclear whether the volatile organic compounds (VOCs) emitted from EPN‐infected cadavers can control RKNs.RESULTSWe investigated the fumigation activity of VOCs released from cadavers infected by five different species of EPNs on RKNs in Petri dishes, and found that VOCs released from Steinernema feltiae (SN strain) and S. carpocapsae (All strain) infected cadavers had a significant lethal effect on second‐stage juveniles (J2s) of Meloidogyne incognita. The VOCs released from the cadavers infected with S. feltiae were analyzed using SPME‐GC/MS. Dimethyl disulfide (DMDS), tetradecane, pentadecane, and butylated hydroxytoluene (BHT), were selected for a validation experiment with pure compounds. The DMDS compound had significant nematicidal activity and repelled J2s. DMDS also inhibited egg hatching and the invasion of tomato roots by J2s. In a pot experiment, the addition of S. feltiae‐infected cadavers and cadavers wrapped with a 400‐mesh nylon net also significantly reduced the population of RKNs in tomato roots after 7 days. The number of root knots and eggs was reduced by 58% and 74.34%, respectively, compared to the control.CONCLUSIONThese results suggested that the VOCs emitted by the EPN‐infected cadavers affected various developmental stages of M. incognita and thus have the potential to be used in controlling RKNs through multiple methods. © 2024 Society of Chemical Industry.

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Selective control of parasitic nematodes using bioactivated nematicides

Cited by 17 publications

References 63 publications

Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1

Nematicidal Activity of 20-Deoxyingenol-3-angelate from Euphorbia peplus Latex Through Protein Kinase C Isotype TPA-1

The marksman: Bioactivated nematicides selectively kill plant‐parasitic nematodes

Volatile organic compounds released from entomopathogenic nematode‐infected insect cadavers for the biocontrol of Meloidogyne incognita

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