Selection of vinasse degrading microorganisms

Abstract

Vinasse is a highly colored effluent with a high pollutant potential when disposed in the environment. Assays for decolorization of vinasse were performed, selecting the fungus Pleurotus sajor-caju CCB 020. The discoloration was cocominant with the increase of the activities of laccase, manganese-peroxidase and peroxidases. P. sajor-caju demonstrated a rise in biomass production (1.06 g 100 ml -1 ), and the enzyme activities such as laccase (varying from 400 to 450 IU l -1 ) reached between the 9th and 10th day of growth and for MnP at the 12th day of cultivation (varying from 60 to 100 IU l -1 ). It was concluded that the system P. sajor-caju/vinasse can be utilized as a bioprocess for color removal and degradation of complex vinasse compounds. It was observed an improvement in the characteristics and detoxification allowing its utilization as reused water, laccase and manganese-peroxidase enzymes production and for fungal biomass production with a high nutritional value.

Expression of manganese peroxidase by Lentinula edodes and Lentinula boryana in solid state and submerged system fermentation

“…After the period of maximum growth the system got into a phase of reduction in the rate of biomass production (characterized as stationary phase), in which secondary metabolism is activated, resulting in the production of bioactive compounds such as phenol oxidative enzymes. Ferreira et al (2010) states that nutritional stress may be one of the responsible factors for MnP synthesis, explaining why this enzyme expression was observed only after the 15 th day (Figure 1). …”

Pretreatment of vinasse from the sugar refinery industry under non-sterile conditions by Trametes versicolor in a fluidized bed bioreactor and its effect when coupled to an UASB reactor

“…They were then diluted to 10% and measured with a spectrophotometer Cole Parmer S2100-UV+ (Cole Parmer, USA) at wavelength of 475 nm [45]. …”

Pleurotusbiomass production on vinasse and its potential use for aquaculture feed

“…The anthropogenic intervention in the environment has revealed the performance of microorganisms in the pursuit of adaptation, when faced with damaging agents, thus becoming a powerful tool for environmental protection that can be further enhanced (Ferreira et al 2010). Industrial effluents represent a source of environmental pollution of rivers, lakes and seas, and it can affect the water quality and the microbial and aquatic flora (Kanu & Achi 2011).…”

“…Between 8 and 15 L of vinasse is produced for each litre of alcohol manufactured (Ferreira et al 2010). It has a dark-brownish colour and is also rich in nutrients.…”

“…The use of industrial by-products such as vinasse for the production of fungal biomass is of great interest from the standpoint of sustainable production of the alcohol industry, as evidenced by Ferreira et al (2010, 2011). Thus, this study aimed to verify the nutritional efficiency and toxicity of mycelia of the fungi Pleurotus sajor-caju, P. albidus, P. flabellatus and P. ostreatus cultivated in vinasse as a food source for Danio rerio and select the most promising species as a potential supplement to commercial feed.…”