Selection of human blastocysts with a high implantation potential based on timely compaction

Mizobe, Yamato; Ezono, Yuji; Tokunaga, Makoto; Oya, Naoto; Iwakiri, Reiko; Yoshida, Naomi; Sato, Yumi; Onoue, Nanase; Miyoshi, Katsuhiko

doi:10.1007/s10815-017-0962-y

Cited by 22 publications

(12 citation statements)

References 39 publications

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“…This process is conserved in all mammalian species studied so far, but the timing varies. The precise timing of the onset and completion of compaction is important for the successful development of both mouse and human embryos ( Mizobe et al, 2017 ; Kim et al, 2017 ; Skiadas et al, 2006 ). Human embryos in which compaction is either initiated prematurely or delayed have a lower potential to form blastocysts (see Glossary, Box 1 ).…”

Section: Compaction and Polarisation: The First Morphogenetic Transfomentioning

confidence: 99%

Mechanisms of human embryo development: from cell fate to tissue shape and back

2020

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Gene regulatory networks and tissue morphogenetic events drive the emergence of shape and function: the pillars of embryo development. Although model systems offer a window into the molecular biology of cell fate and tissue shape, mechanistic studies of our own development have so far been technically and ethically challenging. However, recent technical developments provide the tools to describe, manipulate and mimic human embryos in a dish, thus opening a new avenue to exploring human development. Here, I discuss the evidence that supports a role for the crosstalk between cell fate and tissue shape during early human embryogenesis. This is a critical developmental period, when the body plan is laid out and many pregnancies fail. Dissecting the basic mechanisms that coordinate cell fate and tissue shape will generate an integrated understanding of early embryogenesis and new strategies for therapeutic intervention in early pregnancy loss.

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Section: Compaction and Polarisation: The First Morphogenetic Transfomentioning

confidence: 99%

Mechanisms of human embryo development: from cell fate to tissue shape and back

2020

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“…However, the determination of embryonic development 72 h after insemination might be more appropriate for the "compacting morula" rather than the "morula" in mice. Although previous studies have observed compaction and subsequent embryonic development using time-lapse technology in human embryos, these focused on vacuolization in blastomeres [24], timing of morula formation [22,23,25,26], and patterns of compaction with or without extruded blastomeres [22,27]. There have been, to our knowledge, no previous studies involving the observation of a temporary loosening of compaction during the formation of morula beginning with the initiation of compaction in human embryos [44].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the automatic capture of time-lapse images would provide useful information for embryo selection for subsequent embryo transfer. Previous studies have investigated pronuclear formation [15,16], timing of cleavage [16,17], fragmentation [18][19][20][21], compaction [22][23][24][25][26][27], blastocoel formation [26,28], and the shape of the inner cell mass [29] in human zygotes/embryos using a time-lapse monitoring system. Embryos that cleave early at the first cleavage have a higher potential for subsequent pre-and post-implantation development [17,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Morphokinetic Analysis of Preimplantation Development of Mouse Oocytes Fertilised in Vitro Using a Non-humidifying Incubator With Time-lapse Cinematography (CCM-iBIS)

Watanabe¹,

Ito²,

Shintome³

et al. 2021

Preprint

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Preimplantation development of mouse oocytes fertilised in vitro was assessed in a non-humidified incubator with time-lapse cinematography (CCM-iBIS). The developmental rates of embryos to the 4-cell and blastocyst stages under 5% CO2, 5% O2, and 90% N2 in CCM-iBIS were significantly higher than those under 5% CO2 in air in CCM-iBIS and a conventional CO2 incubator (CPO2-2301). The developmental speed of embryos was much faster in those cultured under lower oxygen tension in CCM-iBIS than in higher oxygen tension in CCM-iBIS and CPO2-2301. Embryonic development was much faster and more synchronised under lower oxygen tension. Non-humidified culture did not affect the development of the embryos. Mouse embryos cultured at lower oxygen tension reached 2-cell at 17 h, 3-cell at 39 h, 4-cell at 40 h, initiation of compaction at 58 h, morula at 70 h, and blastocyst at 82 h after insemination on average. Although compaction partially unravelled with cell division in compacting embryos, it appeared to complete depending on the timing of observation. Observation at a conventional 24-h interval 13 likely misjudges the developmental rate to morula. Determination of embryonic development 72 h after insemination may be more appropriate for “compacting morula” rather than “morula” in mice.

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“…The accumulation of PARD6B also seems to occur about 90 h post fertilization [114]. The human embryo then consists of at least 16 cells and compaction has completed [10,12,103]. This is later than in the mouse, which compacts and polarises during the 8-cell stage [11,20].…”

Section: Internalisation Of the Icm Precursorsmentioning

confidence: 99%

“…While a minority of embryo start compaction as early as during the 8-cell stage, the majority of human embryos begin compaction during the 4 th wave of cleavages, with at least 9 blastomeres and most finish compaction during the 16-cell stage [10]. Embryos compacting less than 80 h after fertilization show higher rate of implantation and live birth than embryos compacting after the 80 h mark [12,101,103]. What controls the timing of compaction remains unclear though.…”

Section: Compactionmentioning

confidence: 99%

Morphogenesis of the human preimplantation embryo: bringing mechanics to the clinics

Firmin

Maître

2021

Seminars in Cell & Developmental Biology

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During preimplantation development, the human embryo forms the blastocyst, the structure enabling uterine implantation. The blastocyst consists of an epithelial envelope, the trophectoderm, encompassing a fluid-filled lumen, the blastocoel, and a cluster of pluripotent stem cells, the inner cell mass. This specific architecture is crucial for the implantation and further development of the human embryo. Furthermore, the morphology of the human embryo is a prime determinant for clinicians to assess the implantation potential of in vitro fertilized human embryos, which constitutes a key aspect of assisted reproduction technology.Therefore, it is crucial to understand how the human embryo builds the blastocyst. As any material, the human embryo changes shape under the action of forces. Here, we review recent advances in our understanding of the mechanical forces shaping the blastocyst. We discuss the cellular processes responsible for generating morphogenetic forces that were studied mostly in the mouse and review the literature on human embryos to see which of them may be conserved. Based on the specific morphological defects commonly observed in clinics during human preimplantation development, we discuss how mechanical forces and their underlying cellular processes may be affected. Together, we propose that bringing tissue mechanics to the clinics will advance our understanding of human preimplantation development, as well as our ability to help infertile couples to have babies.

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Selection of human blastocysts with a high implantation potential based on timely compaction

Cited by 22 publications

References 39 publications

Mechanisms of human embryo development: from cell fate to tissue shape and back

Mechanisms of human embryo development: from cell fate to tissue shape and back

Morphokinetic Analysis of Preimplantation Development of Mouse Oocytes Fertilised in Vitro Using a Non-humidifying Incubator With Time-lapse Cinematography (CCM-iBIS)

Morphogenesis of the human preimplantation embryo: bringing mechanics to the clinics

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