Selected plant essential oils inhibit biofilm formation and
 luxS
 ‐ and
 pfs
 ‐mediated quorum sensing by
 Escherichia coli
 O157:H7

Sharifi, Aram; Fasaei, Bahar Nayeri

doi:10.1111/lam.13673

Cited by 20 publications

(15 citation statements)

References 39 publications

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“…Similarly, it has been reported that luxS and pfs deletion mutants of E. coli inhibit AI-2 production, biofilm formation, and motility ( Yang et al, 2014 ; Wang et al, 2016 ; Zuberi et al, 2017 ). Therefore, the luxS and pfs have been considered important therapeutic drug targets, and many inhibitors against luxS and pfs have been investigated ( Shivaprasad et al, 2021 ; Sharifi and Nayeri Fasaei, 2022 ). The lsr system is liable for AI-2 detection, uptake, and signal transduction in E. coli ( Pereira et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli

et al. 2022

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A new antibacterial strategy based on inhibiting bacterial quorum sensing (QS) has emerged as a promising method of attenuating bacterial pathogenicity and preventing bacterial resistance to antibiotics. In this study, we screened Echinatin (Ech) with high-efficiency anti-QS from 13 flavonoids through the AI-2 bioluminescence assay. Additionally, crystal violet (CV) staining combined with confocal laser scanning microscopy (CLSM) was used to evaluate the effect of anti-biofilm against Escherichia coli (E. coli). Further, the antibacterial synergistic effect of Ech and marketed antibiotics were measured by broth dilution and Alamar Blue Assay. It was found that Ech interfered with the phenotype of QS, including biofilm formation, exopolysaccharide (EPS) production, and motility, without affecting bacterial growth and metabolic activity. Moreover, qRT-PCR exhibited that Ech significantly reduced the expression of QS-regulated genes (luxS, pfs, lsrB, lsrK, lsrR, flhC, flhD, fliC, csgD, and stx2). More important, Ech with currently marketed colistin antibiotics (including colistin B and colistin E) showed significantly synergistically increased antibacterial activity in overcoming antibiotic resistance of E. coli. In summary, these results suggested the potent anti-QS and novel antibacterial synergist candidate of Ech for treating E. coli infections.

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Section: Discussionmentioning

confidence: 99%

Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli

et al. 2022

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“…The inhibition effect of 32 to 256 μg/mL concentrations of synthesized AuNFs on the biofilm of the three bacterial strains tested was determined by a 96-well microplate assay as previously described [ 103 , 104 ]. Briefly, bacterial strains were inoculated in Trypticase Soy Broth (TSB) with 2 % glucose and incubated overnight at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…Then, 100 µL of AuNFs (three wells for each concentration) at different concentrations (MIC to 1/16 MIC) were added into the wells of a 96-well microtiter plate and the plates were incubated further at 37 °C for 24 h. Wells containing bacterial strains and TSB were used as negative controls. The biofilm viability was measured following the previous evaluation protocol [ 103 , 104 ].…”

Section: Methodsmentioning

confidence: 99%

Green Synthesis of Gold Nanoflowers Using Rosmarinus officinalis and Helichrysum italicum Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities

et al. 2022

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This study was concerned with the green synthesis of gold nanoflowers (AuNFs) using the bioactive constituents of Rosmarinus officinalis (rosemary) and Helichrysum italicum (immortelle) extracts, as reducer and stabilizer agents along with the determination of their antibacterial and antibiofilm activity against E. coli, S. aureus, and S. epidermidis. The AuNFs were characterized using STEM, UV–Vis, DLS, ZETA, FESEM-EDX, and FTIR techniques. The antibacterial and antibiofilm activity of the AuNFs were evaluated by microdilution broth and microtiter plate (MTP) tests, respectively. STEM and DLS analysis confirmed the flower-like morphology of gold nanoparticle clusters of R. officinalis-AuNFs (R-AuNFs) and H. italicum-AuNFs (H-AuNFs) with a size of 20–130 nm and 15–90 nm, respectively. The MICs of R-AuNFs were found to be 40 µg/mL for E. coli and S. epidermidis and 160 µg/mL for S. aureus. The MICs of H-AuNFs against all bacterial strains were 20 µg/mL. All tested AuNFs exhibited a strong dose-dependent antibiofilm activity against the test strains, and H-AuNFs was more effective than R-AuNFs. The green synthesis of AuNFs from the rosemary and immortelle extracts can be applied as a potential agent to overcome the growth of biofilm-producing microorganisms in food industries.

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“…In order to investigate the MBC value, 10 μL from wells showing no visible in the microdilution broth test was inoculated onto tryptone soya agar (TSA). The MBC value was defined as the minimum concentration of the compound required to kill 99.9% of the bacteria [70]. These tests were performed in triplicate.…”

Section: The Microdiluition Broth Assaymentioning

confidence: 99%

Antibacterial and anti-biofilm properties of carvacrol alone and in combination with cefixime against Escherichia coli

et al. 2023

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Background Plant-derived compounds can be used as antimicrobial agents in medicines and as food preservatives. These compounds can be applied along with other antimicrobial agents to strengthen the effect and/or reduce the required treatment dose. Results In the present study, the antibacterial, anti-biofilm and quorum sensing inhibitory activity of carvacrol alone and in combination with the antibiotic cefixime against Escherichia coli was investigated. The MIC and MBC values for carvacrol were 250 μg/mL. In the checkerboard test, carvacrol showed a synergistic interaction with cefixime against E. coli (FIC index = 0.5). Carvacrol and cefixime significantly inhibited biofilm formation at MIC/2 (125 and 62.5 μg/mL), MIC/4 (62.5 and 31.25 μg/mL) and MIC/8 (31.25 and 15.625 μg/mL) for carvacrol and cefixime, respectively. The antibacterial and anti-biofilm potential effect of carvacrol confirmed by the scanning electron microscopy. Real-time quantitative reverse transcription PCR revealed significant down-regulation of the luxS and pfs genes following treatment with a MIC/2 (125 μg/mL) concentration of carvacrol alone and of only pfs gene following treatment with MIC/2 of carvacrol in combination with MIC/2 of cefixime (p < 0.05). Conclusions Because of the significant antibacterial and anti-biofilm activity of carvacrol, the present study examines this agent as an antibacterial drug of natural origin. The results indicate that in this study the best antibacterial and anti-biofilm properties are for the combined use of cefixime and carvacrol.

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Selected plant essential oils inhibit biofilm formation and luxS ‐ and pfs ‐mediated quorum sensing by Escherichia coli O157:H7

Cited by 20 publications

References 39 publications

Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli

Novel quorum sensing inhibitor Echinatin as an antibacterial synergist against Escherichia coli

Green Synthesis of Gold Nanoflowers Using Rosmarinus officinalis and Helichrysum italicum Extracts: Comparative Studies of Their Antimicrobial and Antibiofilm Activities

Antibacterial and anti-biofilm properties of carvacrol alone and in combination with cefixime against Escherichia coli

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