Selected autochthonous rhizobia, applied in combination with AM fungi, improve seed quality of common bean cultivated in reduced fertilization condition

Massa, Nadia; Cesaro, Patrizia; Todeschini, Valeria; Capraro, Jessica; Scarafoni, Alessio; Cantamessa, Simone; Copetta, Andrea; Anastasia, Flavio; Gamalero, Elisa; Lingua, Graziano; Berta, Graziella; Bona, Elisa

doi:10.1016/j.apsoil.2020.103507

Cited by 35 publications

(28 citation statements)

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“…For this test, the standard disk diffusion method was used to determine the antibiotics sensitivity of selected rhizobial isolates (Massa et al, 2020 ). The rhizobial culture was grown and spread on YEMA media plates and incubated for 72 h at 28°C.…”

Section: Methodsmentioning

confidence: 99%

Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

Chaudhary

Gera

Shukla

2021

Front. Bioeng. Biotechnol.

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Plant growth-promoting rhizobacteria (PGPR) are root endophytic bacteria used for growth promotion, and they have broader applications in enhancing specific crop yield as a whole. In the present study, we have explored the potential of Rhizobium pusense MB-17a as an endophytic bacterium isolated from the roots of the mung bean (Vigna radiata) plant. Furthermore, this bacterium was sequenced and assembled to reveal its genomic potential associated with plant growth-promoting traits. Interestingly, the root endophyte R. pusense MB-17a showed all essential PGPR traits which were determined by biochemical and PGPR tests. It was noted that this root endophytic bacterium significantly produced siderophores, indole acetic acid (IAA), ammonia, and ACC deaminase and efficiently solubilized phosphate. The maximum IAA and ammonia produced were observed to be 110.5 and 81 μg/ml, respectively. Moreover, the PGPR potential of this endophytic bacterium was also confirmed by a pot experiment for mung bean (V. radiata), whose results show a substantial increase in the plant's fresh weight by 76.1% and dry weight by 76.5% on the 60th day after inoculation of R. pusense MB-17a. Also, there is a significant enhancement in the nodule number by 66.1% and nodule fresh weight by 162% at 45th day after inoculation with 100% field capacity after the inoculation of R. pusense MB-17a. Besides this, the functional genomic annotation of R. pusense MB-17a determined the presence of different proteins and transporters that are responsible for its stress tolerance and its plant growth-promoting properties. It was concluded that the unique presence of genes like rpoH, otsAB, and clpB enhances the symbiosis process during adverse conditions in this endophyte. Through Rapid Annotation using Subsystem Technology (RAST) analysis, the key genes involved in the production of siderophores, volatile compounds, indoles, nitrogenases, and amino acids were also predicted. In conclusion, the strain described in this study gives a novel idea of using such type of endophytes for improving plant growth-promoting traits under different stress conditions for sustainable agriculture.

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Section: Methodsmentioning

confidence: 99%

Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

Chaudhary

Gera

Shukla

2021

Front. Bioeng. Biotechnol.

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“…The final volume of each soil was around 100 cm 3 . The soil samples were analyzed for physical-chemical properties (Table S2) using standard procedures reported in detail in Massa et al, (13). The meteorological data related to the monthly average of maximum and minimum temperatures were identified by the two stations of Djelfa and Ghardaïa of the Algerian National Office of Meteorology.…”

Section: Soil Samplingmentioning

confidence: 99%

“…The genomic DNA was extracted using the DNeasy ® Po-werSoil ® Kit (Qiagen, Milan, Italy), starting from 0.25 g of soil following the manufacturer's instructions and quantified by a fluorometric method according to the Qubit ® 4.0 Fluorimeter protocol. The preparation of the bacterial 16S DNA libraries was performed The soil samples were analyzed for physical-chemical properties (Table S2) using standard procedures reported in detail in Massa et al, (13). The meteorological data related to the monthly average of maximum and minimum temperatures were identified by the two stations of Djelfa and Ghardaïa of the Algerian National Office of Meteorology.…”

Section: Microbiome Characterizationmentioning

confidence: 99%

“…By using the terminology applied for microorganisms inhabiting the human body, these microorganisms are indicated as plant microbiota. These associated bacteria are selected by the release of plant exudates and proliferate outside and inside the plant tissues, affecting plant growth, health, yield as well as the nutritional value of seeds and fruits [5][6][7][8][9][10][11][12][13][14][15]. Thus, they play a relevant role in the improvement of the host's life and fitness.…”

Section: Introductionmentioning

confidence: 99%

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Climatic Zone and Soil Properties Determine the Biodiversity of the Soil Bacterial Communities Associated to Native Plants from Desert Areas of North-Central Algeria

et al. 2021

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Algeria is the largest country in Africa characterized by semi-arid and arid sites, located in the North, and hypersaline zones in the center and South of the country. Several autochthonous plants are well known as medicinal plants, having in common tolerance to aridity, drought and salinity. In their natural environment, they live with a great amount of microbial species that altogether are indicated as plant microbiota, while the plants are now viewed as a “holobiont”. In this work, the microbiota of the soil associated to the roots of fourteen economically relevant autochthonous plants from Algeria have been characterized by an innovative metagenomic approach with a dual purpose: (i) to deepen the knowledge of the arid and semi-arid environment and (ii) to characterize the composition of bacterial communities associated with indigenous plants with a strong economic/commercial interest, in order to make possible the improvement of their cultivation. The results presented in this work highlighted specific signatures which are mainly determined by climatic zone and soil properties more than by the plant species.

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“…Indeed, these two components are strongly involved in the biogeochemical cycle and improvement of plant growth (Lu & Hedin, 2019). In addition, numerous research studies have shown that the development and productivity as well as the quality of common bean pods are strongly related to the symbiosis that the plant establishes with beneficial soil microorganisms including nitrogen-fixing bacteria and endomycorrhizal fungi with vesicles and arbuscular (Massa et al, 2020). In this context, this paper will allow the comparison of the yields of Phaseolus vulgaris L. in two plots with or without nitrogen fertilization.…”

Section: Introductionmentioning

confidence: 99%

Effects of Nitrogen Fertilization on Mycorrhizal Infection, Nodulation and Growth of Phaseolus vulgaris L.

Mansouri

Kheloufi

Belatreche

et al. 2020

Contemporary Agriculture

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SummaryThe common bean (Phaseolus vulgaris L.) has been the subject of a number of studies and has been described by many authors as a legume with low nitrogen fixing potential compared to other legume species. The first objective of this study is to assess the development and growth of P. vulgaris L. var. Djedida and its yield at different developmental stages (vegetative, flowering and fruiting), in the presence and absence of nitrogen fertilizer (Urea 46) in an arid climate and in a soil containing high level of CaCO3 and assimilable phosphorus. The second objective is to carry out a survey on nodulation, number of spores and mycorrhizal infection under these conditions. The results showed that the nitrogen fertilizer amendment is mainly significant (p< 0.0001) for various morphological parameters. Indeed, great improvement was observed on the majority of the morphological parameters with considerable percentages. However, no significant effect was reported for the roots length. In addition, a negligible number of nodules were obtained in the plot without fertilization and no significant effect on the number of spores was recorded. Furthermore, the effect of fertilization on the arbuscular intensity (a%, A%) and on mycorrhizal colonization (M%, m%) of the roots was found to be significant. The soil in the arid region of Biskra showed significant mycorrhizogenic potential, although the conditions in this region were not very favorable for their development, which could constitute an effective biological resource to improve the tolerance of host plants to biotic and abiotic constraints.

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Selected autochthonous rhizobia, applied in combination with AM fungi, improve seed quality of common bean cultivated in reduced fertilization condition

Cited by 35 publications

References 35 publications

Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

Deciphering the Potential of Rhizobium pusense MB-17a, a Plant Growth-Promoting Root Endophyte, and Functional Annotation of the Genes Involved in the Metabolic Pathway

Climatic Zone and Soil Properties Determine the Biodiversity of the Soil Bacterial Communities Associated to Native Plants from Desert Areas of North-Central Algeria

Effects of Nitrogen Fertilization on Mycorrhizal Infection, Nodulation and Growth of Phaseolus vulgaris L.

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