Selectable Marker Genes Engineered for Specific Expression in Target Cells for Plant Transformation

Özcan, Sebahattin; Firek, Simon; Draper, John

doi:10.1038/nbt0293-218

Cited by 22 publications

(13 citation statements)

References 22 publications

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“…As expected from previous studies (e.g. Ozcan et al, 1993) which have utilised the CaMV35S promoter, the GUS ($358) plants displayed a range of constitutive GUS activities with peak levels greater than 60 pmoles 4MU h -1 gg-1 of protein (S358) were chosen for analysis. GUS activity was measured in extracts produced from total leaf tissue.…”

Section: Endoplasmic Reticulum-targeted G Us ($358) Is Activementioning

confidence: 99%

Endoplasmic reticulum targeting of active modified β-glucuronidase (GUS) in transgenic tobacco plants

Firek

Whitelam

Draper

1994

Transgenic Research

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We have investigated targeting to the endoplasmic reticulum (ER) of wild-type GUS and a modified form (GUS S358) by making an N-terminal fusion of the beta-glucuronidase (GUS) enzyme with the wheat alpha-amylase signal peptide. In vitro studies demonstrated that the modified GUS (S358) lacked the glycosylation site present within the wild-type enzyme. Analysis of transgenic tobacco plants revealed that the modified GUS enzyme retained activity upon passage to the ER. When further experiments were carried out to determine the cellular location of the modified GUS enzyme, it was found that (contrary to expectation) the majority of GUS activity was retained within the cell and was not secreted to the cell surface via the default pathway. The data indicated that the modified GUS enzyme is an unsuitable reporter enzyme for studying protein secretion.

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Section: Endoplasmic Reticulum-targeted G Us ($358) Is Activementioning

confidence: 99%

Endoplasmic reticulum targeting of active modified β-glucuronidase (GUS) in transgenic tobacco plants

Firek

Whitelam

Draper

1994

Transgenic Research

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“…Considering that AoPR1-directed GUS gene expression was mainly confined to wound sites, it was obvious that the AoPR1 promoter was transcriptionally more active, on a per cell basis, following wounding than CaMV35S promoter [11]. Although the AoPR1 promoter showed strong expression at wound sites and in developing callus tissue, it was virtually silent in non-wounded leaves, roots and tubers (the latter in the case of potato) tissue from the transgenic plants [16,17]. We report here the use of the AoPR1 promoter to limit the expression of a Bt gene to wound sites caused by insect invasion.…”

Section: Introductionmentioning

confidence: 98%

Expression of Cry1Ac in Transgenic Tobacco Plants Under the Control of a Wound-Inducible Promoter (AoPR1) Isolated from Asparagus officinalis to Control Heliothis virescens and Manduca sexta

et al. 2009

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Expression of cry1Ac gene from Bacillus thuringiensis (Bt) was evaluated under the control of a wound-inducible AoPR1 promoter from Asparagus officinalis in transgenic tobacco plants. The leaves of transgenic plants were mechanically wounded to evaluate the activity of the AoPR1 promoter in driving the expression of Cry1Ac protein at the wound site. Our results indicate that mechanical wounding of transgenic plants was effective in inducing the expression of Cry1Ac protein. As a result of this induction, the accumulated levels of Cry1Ac protein increased during 6-72 h post-wounding period. The leaves of transgenic tobacco plants were evaluated for resistance against Heliothis virescens and Manduca sexta in insect bioassays in two different ways. The detached tobacco leaves were either fed directly to the insect larvae or they were first mechanically wounded followed by a 72 h post-wounding feeding period. Complete protection of mechanically wounded leaves of transgenic plants was observed within 24 h of the bioassay. The leaves of transgenic plants fed directly (without pre-wounding) to the larvae achieved the same level of protection between 24 and 72 h of the bioassay.

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“…This system was successfully used in tobacco first time for suppression of npt II wound inducible promoter AoPRi 28 . This system allowed selection of transformed cells and less or negligible expression of npt II after selection.…”

Section: Temporary Expression Of Marker Genementioning

confidence: 99%

Approaches for Biological Improvement of Ecofriendly Crops

Priyadarshi¹,

Shukla²,

Borse³

2013

J Biol Sci Opin

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Selectable Marker Genes Engineered for Specific Expression in Target Cells for Plant Transformation

Cited by 22 publications

References 22 publications

Endoplasmic reticulum targeting of active modified β-glucuronidase (GUS) in transgenic tobacco plants

Endoplasmic reticulum targeting of active modified β-glucuronidase (GUS) in transgenic tobacco plants

Expression of Cry1Ac in Transgenic Tobacco Plants Under the Control of a Wound-Inducible Promoter (AoPR1) Isolated from Asparagus officinalis to Control Heliothis virescens and Manduca sexta

Approaches for Biological Improvement of Ecofriendly Crops

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