Seleção de agentes alternativos ao ágar para propagação de plântulas de Cattleya loddigesii Lindley (Orchidaceae)

Galdiano, Renato Fernandes; Mantovani, Cibele; Lemos, Eliana Gertrudes de Macedo

doi:10.5039/agraria.v7isa1930

Cited by 4 publications

References 14 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

View full text Add to dashboard Cite

Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

show abstract

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

View full text Add to dashboard Cite

show abstract

In vitro propagation and conservation of Cattleya tigrina A. Rich

et al. 2022

View full text Add to dashboard Cite

Cattleya tigrina A. Rich has been suffering heavy losses in its natural habitat and it is now included in the list of plants that are vulnerable to extinction. The development of in vitro propagation and conservation methodologies, as well as acclimatization, are considered important for species at the risk of extinction, as they promote the multiplication and conservation of the species, hence avoiding the loss of their genetic variability. The present study established the protocol of micropropagation and the in vitro conservation of C. tigrina. For the in vitro propagation, the study tested two volumes of the MS medium and two medium consistencies (stationary liquid and semi-solid). For acclimatization, the substrate mixtures containing pine bark, charcoal, vermiculite, and coconut coir were analyzed. For the in vitro conservation, different concentrations of the salts were tested in the MS medium, together with the osmotic regulators (sucrose, mannitol, and sorbitol), and at two temperatures (18 and 25 °C). The results obtained inferred that the semi-solid medium was superior to the stationary liquid medium in the variables of survival and the presence of roots, while the liquid medium was superior to the semi-solid medium in the number of shoots. For acclimatization, pine bark was the substrate where the plants developed an improved height, with sprouting, and rooting. The conservation was satisfactory and the plants remained viable for a period of 730 days, with the MS medium with 25% of the salts, and at temperatures of 18 ºC or 25 ºC. The plants were propagated in the stationary liquid MS medium (10 mL) and the semi-solid medium (25 mL), while they were acclimatized in pine bark and preserved in the MS medium with 25% of the salts (18 ºC or 25 ºC).

show abstract

AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus

Rabaiolli

Reiniger

Stefanel

et al. 2017

CERNE

View full text Add to dashboard Cite

ABSTRACT:The golden trumpet tree Handroanthus chrysotrichus (Bignoniaceae) is an important species, owing to its economic, ecological, medicinal, and ornamental value. However, its production is hindered due to lack of seed availability and viability improved methods of vegetative propagation are needed. Therefore, we evaluated the effect of agar on in vitro rhizogenesis of H. chrysotrichus shoots and the subsequent ex vitro acclimatization of the plants obtained. In the in vitro rhizogenesis assay, we cultured the micropropagated shoots on half-strength nutritive Woody Plant Medium (½WPM) supplemented with 30 g . L -1 sucrose, 50 mg . L -1 myoinositol, 30 cm³ of vermiculite, and one of the three agar concentrations (0, 3.5, or 7 g . L -1 ), and after 30 d, we assessed the shoots for root production and leaf number. Thereafter, the plants were subjected to a 49-d ex vitro acclimatization period, which consisted of two stages in a growth room and one stage in a greenhouse; we assessed plant survival, leaf number and increases in stem height at the end of each stage. We found that the addition of agar to the growth medium was not necessary to induce adventitious rooting, especially with respect to secondary roots, and that plants grown on medium without agar had the highest number of leaves after acclimatization. Therefore, agar actually hindered both in vitro rhizogenesis and ex vitro acclimatization. The ex vitro acclimatization result in high survival and resumption of growth and development of H. chrysotrichus micropropagated plants, and this step is not affected by agar on in vitro rhizogenesis. AGAR NÃO AFETA A RIZOGÊNESE IN VITRO E A ACLIMATIZAÇÃO EX VITRO DE Handroanthus chrysotrichus RESUMO:Handroanthus chrysotrichus (Mart. ex DC.) J. Mattos (Bignoniaceae), conhecida como ipê-amarelo, é uma espécie dotada de importância econômica, ecológica e ornamental. No presente trabalho o objetivo foi avaliar o efeito do ágar na rizogênese in vitro e na subsequente aclimatização ex vitro, bem como a eficiência deste processo em relação à sobrevivência e à retomada do crescimento e desenvolvimento das plantas micropropagadas de H. chrysotrichus. Na rizogênese in vitro, brotações micropropagadas na ausência de reguladores de crescimento foram cultivadas in vitro por 30 dias, em meio nutritivo WPM cujas concentrações de sais foram reduzidas à metade (½WPM), acrescido de 30 g . L -1 de sacarose, 50 mg . L -1 de mio-inositol e 30 cm³ de vermiculita, na presença de diferentes concentrações de ágar (0; 3,5 ou 7 g . L -1 ) e ausência de fitorreguladores. Decorrido esse período, as plantas provenientes dos tratamentos do ensaio de rizogênese foram submetidas à aclimatização ex vitro, processo composto por três etapas e duração de 49 dias. O ágar foi dispensável na indução de raízes adventícias, principalmente no que diz respeito às secundárias. Igualmente, na aclimatização, plantas provenientes de cultivo na ausência de ágar apresentaram melhor desempenho, em especial no que diz respeito ao número de folhas. A ausência de ága...

show abstract

Seleção de agentes alternativos ao ágar para propagação de plântulas de Cattleya loddigesii Lindley (Orchidaceae)

Cited by 4 publications

References 14 publications

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

In vitro propagation and conservation of Cattleya tigrina A. Rich

AGAR DOES NOT AFFECT IN VITRO RHIZOGENESIS AND EX VITRO ACCLIMATIZATION OF Handroanthus chrysotrichus

Contact Info

Product

Resources

About