Seeds of Endangered<i>Harrisia fragrans</i>form Persistent Soil Seed Banks and Withstand Orthodox Storage Conditions

Goodman, Joie; Walters, D.F.; Bradley, Keith; Maschinski, Joyce; Salazar, Ana

doi:10.2985/026.018.0111

Cited by 13 publications

(22 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results agree with those of Goodman et al [23] who found that seeds of Harrisia fragrans, a globally endangered cactus, can withstand orthodox storage conditions. Our results also agree with studies on cactus species from coastal and arid regions of Brazil, which exhibit orthodox seed behavior [25].…”

Section: Discussionsupporting

confidence: 83%

“…After desiccation, we sealed seeds in foil packs and froze them at -20°C for one week or 28 weeks. Because scarification with sulfuric acid has been shown to be an effective method to break dormancy of Harrisia fragrans (Cactaceae) seeds [23] after the treatments we soaked the seeds in 1N sulfuric acid (H 2 SO 4 ) for one minute, rinsed them twice with tap water, and then soaked them in tap water for 15 minutes. We sowed the scarified seeds in Petri dishes on filter paper and dampened with plant preservative mixture water (Caisson ® ).…”

Section: Seed Orthodox Storagementioning

confidence: 99%

See 1 more Smart Citation

Ex-situ Seed Conservation of Endangered Key Tree Cactus (Pilosocereus robinii)

Maschinski¹

2013

J Biodivers Endanger Species

View full text Add to dashboard Cite

Ex-situ seed collections are fundamental to the conservation of threatened and endangered plant species. We tested whether or not seeds of the U.S. endangered tree cactus, Pilosocereus robinii, withstand orthodox storage conditions of low humidity (25%RH) and freezing temperatures (-20°C). Mean final germination of fresh seeds and seeds stored under low humidity and low temperature for 1 and 28 weeks ranged from 92 to 96%. Mean final germination did not differ significantly across storage conditions. Seed germination rate exhibited small, but significant differences across storage conditions. Seeds stored under dry conditions of low humidity and ambient temperature germinated significantly faster than dry-stored seeds maintained at freezing temperatures and fresh seeds. Because orthodox methods of drying and freezing can be used to store seeds of Pilosocereus robinii, ex-situ seed banking can effectively assist the conservation and regeneration of this endangered species.

show abstract

Section: Discussionsupporting

confidence: 83%

Section: Seed Orthodox Storagementioning

confidence: 99%

Ex-situ Seed Conservation of Endangered Key Tree Cactus (Pilosocereus robinii)

Maschinski¹

2013

J Biodivers Endanger Species

View full text Add to dashboard Cite

show abstract

“…This reduction of MGT over storage time can be attributed to the possible formation of fissures in the seeds tegument, which can facilitate the soaking process (Salomão ; Goodman et al . ; Salazar et al . ).…”

Section: Discussionmentioning

confidence: 96%

“…After being stored at low temperature conditions, the seeds of some species can germinate significantly faster than non-stored seeds or seeds stored at room temperature, as we observed for seeds of M. sergipensis and M. zehntneri stored in a cold chamber and a freezer. This reduction of MGT over storage time can be attributed to the possible formation of fissures in the seeds tegument, which can facilitate the soaking process (Salomão 2002;Goodman et al 2012;Salazar et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Seed storage of Brazilian cacti species in different threat categories

Santos

Hassemer

Meiado

2018

Plant Species Biology

View full text Add to dashboard Cite

Cactaceae is considered the fifth most endangered taxonomic group. In light of this, the aim of this study was to evaluate the efficiency of different low‐temperature storage techniques in maintaining the viability of seeds of cacti in different threat categories. Seeds of six cacti taxa were stored in a cold chamber (8°C), a freezer (−5°C), in liquid nitrogen (−196°C) and at room temperature (25–27°C) for a period of 0, 1, 3, 6, 9 and 13 months. At each evaluation interval we removed a seed sample for each taxon studied, which was distributed into four repetitions of 25 seeds maintained at room temperature under 12‐h light/dark photoperiods. We evaluated the germinability, mean germination time and synchronization index. Most of the studied taxa presented germinability of above 50%, which was influenced by time and by storage temperatures. Also, most taxa stored at room temperature presented a significant reduction in germinability, whereas almost all taxa showed maintenance of the seed viability when stored in a cold chamber, a freezer or liquid nitrogen. This response can be justified by the reduction of the seed metabolism and the degradation of the reserve compounds of the seeds while at lower temperatures. Our results indicate that storage at low temperatures is an effective method for the conservation of cacti seeds and can be used for the formation of artificial seed banks of threatened cacti species.

show abstract

“…; Cheib & Garcia ; Goodman et al . ; Álvarez‐Espino et al . ); however, most studies did not extend beyond 2 years.…”

Section: Introductionmentioning

confidence: 99%

Ecological longevity of Polaskia chende (Cactaceae) seeds in the soil seed bank, seedling emergence and survival

et al. 2017

View full text Add to dashboard Cite

Soil seed banks are essential elements of plant population dynamics, enabling species to maintain genetic variability, withstand periods of adversity and persist over time, including for cactus species. However knowledge of the soil seed bank in cacti is scanty. In this study, over a 5-year period we studied the seed bank dynamics, seedling emergence and nurse plant facilitation of Polaskia chende, an endemic columnar cactus of central Mexico. P. chende seeds were collected for a wild population in Puebla, Mexico. Freshly collected seeds were sown at 25 °C and 12-h photoperiod under white light, far-red light and darkness. The collected seeds were divided in two lots, the first was stored in the laboratory and the second was use to bury seeds in open areas and beneath a shrub canopy. Seeds were exhumed periodically over 5 years. At the same time seeds were sown in open areas and beneath shrub canopies; seedling emergence and survival were recorded over different periods of time for 5 years. The species forms long-term persistent soil seed banks. The timing of seedling emergence via germination in the field was regulated by interaction between light, temperature and soil moisture. Seeds entered secondary dormancy at specific times according to the expression of environmental factors, demonstrating irregular dormancy cycling. Seedling survival of P. chende was improved under Acacia constricta nurse plants. Finally, plant facilitation affected the soil seed bank dynamics as it promoted the formation of a soil seed bank, but not its persistence.

show abstract

Seeds of EndangeredHarrisia fragransform Persistent Soil Seed Banks and Withstand Orthodox Storage Conditions

Cited by 13 publications

References 39 publications

Ex-situ Seed Conservation of Endangered Key Tree Cactus (Pilosocereus robinii)

Ex-situ Seed Conservation of Endangered Key Tree Cactus (Pilosocereus robinii)

Seed storage of Brazilian cacti species in different threat categories

Ecological longevity of Polaskia chende (Cactaceae) seeds in the soil seed bank, seedling emergence and survival

Contact Info

Product

Resources

About