Seed Viability Determinations in Cabbage Utilizing Sinapine Leakage and Electrical Conductivity Measurements

Hill, H. J.; Taylor, A.G.; Huang, Xiao-hua

doi:10.1093/jxb/39.10.1439

Cited by 15 publications

(8 citation statements)

References 12 publications

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“…Conductivity is often cited as a useful vigour indicator as it is thought to reflect the general state of membrane integrity within the seeds (Oliveira et al, 1984). However, results can be conflicting and it is not always an accurate predictor for B. oleracea seed vigour (Hill et al, 1988). The present results also raise questions about the usefulness of conductivity as a measure of Brassica seed vigour, as it is not correlated with other measures of seed performance such as germination under stress (Table 1).…”

Section: mentioning

confidence: 65%

Quantitative genetic analysis of seed vigour and pre‐emergence seedling growth traits in Brassica oleracea

Bettey¹,

Finch‐Savage²,

King³

et al. 2000

New Phytologist

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The ability of seeds to germinate and establish seedlings in a predictable manner under a range of conditions has a direct contribution to the economic success of commercial crops, and should therefore be considered in crop improvement. We measured traits associated with seed vigour and pre-emergence seedling growth in a segregating population of 105 doubled haploid Brassica oleracea lines. The germination traits measured were : mean germination times for unstressed germination ; germination under water stress or germination after a heat treatment ; and conductivity of seed leachate. The seedling growth traits measured were : seed weight ; seedling growth rate ; and seedling size at the end of the exponential growth phase. There were some correlations, notably among germination traits, and between seed weight and pre-emergence seedling growth. Heritability of the various traits was typically in the 10-15% range, with heritability of conductivity and mean germination time under water stress 25 and 24% respectively. Collectively the results indicate that germination and pre-emergence seedling growth are under separate genetic control. Quantitative trait loci analyses were carried out on all measurements and revealed significant loci on linkage groups O1, O3, O6, O7 and O9. We suggest that genes at these loci are important in determining predictable seed germination and seedling establishment in practice.

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Section: mentioning

confidence: 65%

Quantitative genetic analysis of seed vigour and pre‐emergence seedling growth traits in Brassica oleracea

Bettey¹,

Finch‐Savage²,

King³

et al. 2000

New Phytologist

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“…Sinapine thiocyanate (C 16 H 24 NO 5 SCN) forms through the combination of sinapine and SCN − (Harloff et al, 2012) and is a key marker compound for the qualitative and quantitative detection of sinapine (Thiyam et al, 2009; Huang et al, 2011). The leakage of sinapine from seeds indicates changes in seed viability, because sinapine leaks when the cell membrane is damaged, and sinapine leakage is negatively linearly correlated to germination rates (Hill et al, 1988; Taylor et al, 1991, 1993; Bettey et al, 2000). Seed viability can be predicted by measuring the sinapine leakage by optical absorption; however, this method can only distinguish seeds with distinct differences in viability, not those with subtle differences.…”

mentioning

confidence: 99%

Rapid and Nondestructive Determination of Radish Seed Viability by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry

Cui

et al. 2018

Crop Science

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Traditional methods of seed viability evaluation based on germination or detecting physiological indicators have several disadvantages, such as time consumption, seed destruction, and skill requirements et al. In this study, a nondestructive method based on surface desorption atmospheric pressure chemical ionization mass spectrometry (DAPCI–MS) was developed to evaluate radish [Raphanus sativus (L.) Domin] seed viability by detecting the sinapine that leakage from the interior of the seed to the coat. The signal intensity of the detected sinapine in seed coat by DAPCI–MS was significantly negatively correlated with the seed viability. One seed could be analyzed in an average of 4.4 s. We establish a new method to test radish seed viability based on DAPCI‐MS. This is a highly sensitive and high‐throughput method for detection without sample pretreatment. In addition, this study also provides new insights into the evaluation of seed viability in other plants.

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“…The absorbance at these wavelengths was considered to be proportional to the sinapine concentration in solution. A 26-fold increase in leakage was measured from heat-killed compared to viable seeds after an 8-h soak (Hill et al, 1988). Six seedlots from B. oleracea L. Capitata group, B. oleracea L. Botrytis group., B. napus L., and Erysimum hieracifolium L. were deteriorated by increasing seed moisture content to 20% (fresh weight basis) and incubating at 45C for 48 h (Taylor et al, 1991).…”

mentioning

confidence: 99%

Sinapine Leakage from Brassica Seeds

Taylor¹,

Paine²,

Paine³

1993

jashs

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The purpose of this project was to study factors that influence the leakage rate and develop methods to enhance leakage of sinapine from Brassica seeds. Six seedlots (two seedlots of one cultivar each of cabbage (Brassica oleracea L., Capitata group), broccoli, and cauliflower (B. oleracea L., Botrytis group) were studied. Leakage was quantified spectrophotometrically by measuring the absorbance of the soak water at 330 nm. The onset of sinapine leakage was determined from single seeds by the visual presence (yellow soak water) caused by soaking seeds in a biological buffer adjusted to pH 10. The leakage pattern from heat-killed seeds of all seedlots was sigmoidal with a distinct lag phase followed by a rapid efflux and final slower rate. The duration of the lag phase and the total amount of sinapine leaked after 24 hours was not the same for all seedlots after adjusting for seed count, seed weight, or sinapine content. Therefore, another factor was responsible for differences measured in leakage. Embryos or seeds with cracked testas leaked faster than intact seeds, and the leakage pattern without testa integrity was biphasic. From these studies, we conclude that the testa was a major factor regulating sinapine leakage. Pretreating heat killed seeds, with up to 1.0% NaOCl for 10 minutes, accelerated the onset of leakage. The time for 50 percent of the seeds to leak (T50) decreased as NaOCl concentration increased. Leakage uniformity, as measured by the standard deviation of the distribution, generally increased as NaOCl concentration increased. The sigmoidal leakage pattern from heat-killed Brassica seeds may be attributed to seedcoat cracking associated with imbibitional swelling. A NaOCl pretreatment may have increased the permeability of the testa and, thereby, enhanced the leakage rate.

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Seed Viability Determinations in Cabbage Utilizing Sinapine Leakage and Electrical Conductivity Measurements

Cited by 15 publications

References 12 publications

Quantitative genetic analysis of seed vigour and pre‐emergence seedling growth traits in Brassica oleracea

Quantitative genetic analysis of seed vigour and pre‐emergence seedling growth traits in Brassica oleracea

Rapid and Nondestructive Determination of Radish Seed Viability by Surface Desorption Atmospheric Pressure Chemical Ionization Mass Spectrometry

Sinapine Leakage from Brassica Seeds

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