Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

Bae, Kee Hwa; Yoon, Eui Soo

doi:10.3329/ptcb.v25i1.24110

Cited by 3 publications

(2 citation statements)

References 20 publications

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“…A few related species from the genus Lychnis have been already successfully introduced to in vitro cultures: L. senno (Chen et al 2006a, b), L. wilfordii (Bae et al 2014), L. fulgens, and L. cognata (Bae and Yoon 2015). In this study, different cultures of L. flos-cuculi have been obtained for the first time.…”

Section: Discussionmentioning

confidence: 89%

“…In contrast, a single L. senno shoot tip explant resulted in formation of at most three axillary shoots on MS medium containing BAP 5.0 mg l −1 with NAA 0.5 mg l −1 . The shoot cultures of the other species, namely L. cognata, L. fulgens, and L. wilfordii, were all established through indirect organogenesis, therefore the efficiency of multiplication coefficient is difficult to compare (Bae et al 2014;Bae and Yoon 2015). For L. flos-cuculi, the presence of auxins in the media was not crucial in the process of rhizogenesis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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Lychnis flos-cuculi L. is a species containing ecdysteroids, triterpenoid saponins, flavonoids, and phenolic acids, and therefore is a plant of potential medicinal value. In the presented research, diverse in vitro cultures of this taxon were developed to obtain the uniform material capable of producing ecdysteroids, including micropropagated plantlets, shoot cultures, liquid agitated whole plant cultures with fast-growing roots, and callus. A protocol of micropropagation through axillary bud formation was established using plant growth regulators at different concentrations and combinations. All the variants of plant growth regulator supplementation significantly affected a shoot proliferation rate ranging from 8 to 16 plantlets per explant, depending on the medium; DNA content of all the studied plant materials was similar. The thin-layer chromatography analysis of the extracts revealed the presence of ecdysteroids in every plant material apart from callus. The content of 20-hydroxyecdysone and polypodine B was evaluated by high-performance liquid chromatography. Agitated plantlets were in vitro cultures that efficiently formed abundant root biomass with significant concentrations of ecdysteroids. In vitro-derived adventitious roots contained twofold higher content of ecdysteroids than those of intact plants. The organs of flowering in vitro-propagated plants, transferred to experimental plot, contained twice as much ecdysteroids when compared to the organs of plants from the natural site, among which flowers were the richest in ecdysteroids. The results revealed that adventitious roots from L. flos-cuculi agitated cultures can be considered as an alternative biotechnological source of biomass rich in pharmaceutically active ecdysteroids. Key message Diverse types of Lychnis flos-cuculi in vitro cultures have been developed. HPLC analysis demonstrated much higher ecdysteroid content in cultures and regenerated plants when compared to natural site plants. Keywords Micropropagation • Callus • 20-Hydroxyecdysone • Polypodine B • Agitated plant cultures • Genome size Abbreviations 2,4-D 2,4-Dichlorophenoxyacetic acid 20HE 20-Hydroxyecdysone BAP N 6-benzylaminopurine Dic Dicamba (3,6-dichloro-2-methoxybenzoic acid) IAA Indole-3-acetic acid Kin Kinetin (N 6-furfuryladenine) MS Murashige & Skoog medium NAA 1-Naphthaleneacetic acid PGRs Plant growth regulators Pic Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) polB Polypodine B (5β,20-dihydroxyecdysone) TDZ Thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea TLC Thin-layer chromatography Communicated by Ali R. Alan.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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Morphological, cytogenetic, and phytochemical estimation of Lychnis flos-cuculi L. cell cultures: can cell biomass be a source of bioactive compounds?

Hermosaningtyas,

Kruszka,

Juszczyk

et al. 2024

Plant Cell Tiss Organ Cult

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The ragged robin (Lychnis flos-cuculi L.) from the Caryophyllaceae family contains flavonoids, phenolic acids, flavonoids, triterpenoid saponins, and ecdysteroids, making it potential for biological and medicinal application. Plant cell culture technologies could be beneficial for obtaining low-cost biomass sources with valuable phytochemicals sustainably. The current study reports the induction and proliferation of ragged robin cell culture and evaluation of various plant growth regulators (PGRs) impact on the secondary metabolites profile using UPLC-HRMS/MS. Callus was initiated from different explants on the Murashige and Skoog medium with various combinations and concentrations of PGRs. It was found that prolonged proliferation on MS medium enriched with a combination of 1.0 mg l− 1 2,4-Dichlorophenoxyacetic acid and 0.1 mg l− 1 N6-furfuryl adenine (Kinetin) yields the maximum biomass with 504.05% growth index. The results of flow cytometric analysis suggested no genetic alteration between differentiated and dedifferentiated cultures. Furthermore, the UPLC-HRMS/MS result distinguished 101 significant signals that were manually identified as ecdysteroids (16 metabolites), flavonoids (11 metabolites), phenolic acids (10 metabolites), triterpene saponins and sapogenins (53 metabolites), and some primary metabolites. Our study demonstrates that the cell culture of L. flos-cuculi displays significant differences in its phytochemical makeup to plants cultivated in vitro. Moreover, the type of growth regulators used for callus cultures was identified to cause substantial shifts in the identified metabolites. Graphical Abstract

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Cold Stratification, Gibberellic Acid, and Priming Treatments to Promote Germination of <i>Lychnis wilfordii</i> (Regel) Maxim. Seeds

Kim¹,

Cho²,

Cho³

et al. 2020

Flower Res. J.

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Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

Cited by 3 publications

References 20 publications

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Morphological, cytogenetic, and phytochemical estimation of Lychnis flos-cuculi L. cell cultures: can cell biomass be a source of bioactive compounds?

Cold Stratification, Gibberellic Acid, and Priming Treatments to Promote Germination of <i>Lychnis wilfordii</i> (Regel) Maxim. Seeds

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