Section 1B: Rh flow cytometryCoordinatorˈs report.Rhesus index and antigen density: an analysis of the reproducibility of flow cytometric determination

The DAU allele cluster of the RHDgene

“…16,20 The secondary antibody was goat anti-human IgG, Fab-fragment, fluorescein isothiocyanate (FITC)-conjugated (Jackson Immunoresearch, supplied by Dianova, Hamburg, Germany). For the DAU-3 sample, which had a positive direct agglutination test, the background fluorescence was determined by incubating the sample with secondary antibody only.…”

DNB: a partial D with anti-D frequent in Central Europe

“…An antigen density of 5908 antigens per cell and a Rhesus index 13,18 (RI) of 0.02 were determined in RIR-2. These results were comparable to those obtained with a DNU sample (8073 antigens per cell, RI 0.06) and a CcDee DIV type 3 sample (4544 antigens per cell, RI 0).…”

Avaliação de reagentes anti-D na detecção dos antígenos D fraco e D parcial

“…[20][21][22] O anticorpo secundário utilizado foi um anticorpo IgG antihumano produzido em cabra, fragmento Fab, conjugado com fluoresceína (FITC). A fluorescência obtida nas amostras RhD fraco foi comparada com a de hemácias controles com o fenótipo RhD-positivo R1r (DCcee).…”