Secretory expression of a novel human spermatozoa antigen in E. coli and its application to a protein chip

Wang, Mingzhu; Qiu, Zhuolin; Cai, Xiangsheng; Li, Jingjing; She, Miaoqin; Xu, Yuanfeng; Wu, Yixuan

doi:10.1007/s10529-017-2377-3

Cited by 2 publications

(2 citation statements)

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“…In addition, the results obtained with the MAR test were also comparable with the biochip approach (Xu et al 2020). This type of technology was, therefore, suggested as an important tool for the screening of ASA in serum in large cohorts of patients (Wang et al 2017, Xu et al 2020.…”

Section: R58mentioning

confidence: 58%

“…The recently validated protein biochip, a microarraybased technology, which takes advantage of the highthroughput methodology with minimal input of sample and providing extremely high sensitive and specific results, has shown significant consistency with ELISA in serum ASA detection (Wang et al 2017, Xu et al 2020). In addition, the results obtained with the MAR test were also comparable with the biochip approach (Xu et al 2020).…”

Section: R58mentioning

confidence: 99%

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The impact of antisperm antibodies on human male reproductive function: an update

2021

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Immune infertility occurs due to the presence of antisperm antibodies (ASA). This type of infertility has a relatively low prevalence (2.6-6.6% in infertile men), and its etiology, risk factors, targets and consequences for male fertility are not completely understood. While it is largely accepted that abnormalities in the blood-testis barrier and/or blood-epididymal barrier are the main factors behind its etiology, and that sperm motility is the most frequently reported altered parameter, few are the well-defined risk factors and ASA targets only now started to be disclosed, with proteins involved in sperm-oocyte interaction rising as the most significant. The development of potential treatments is also limited, being the corticosteroids the more promising. Overall, there are still many knowledge gaps related to immune infertility. With this review we aimed to gather all the information collected from studies developed in humans in the last decade. Despite the controversial results/inconsistencies, that are not only a result from the complexity of mechanisms/variables involved in ASA infertility, but also from the technical approaches to assess ASA and the lack of a consensus regarding the thresholds to be used, this manuscript aims to bring a fresh update on the field. It has become clear that, to obtain more/reliable data, there is a need to assess ASA in all the routine seminal analysis, following WHO recommendations. In this way it will be possible to obtain consistent and comparable information, that can add to current knowledge. Additionally, multicentric studies with large cohorts are also missing, and future research should take this into consideration.

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Section: R58mentioning

confidence: 58%