Secretome of Olfactory Mucosa Mesenchymal Stem Cell, a Multiple Potential Stem Cell

Abstract

Nasal olfactory mucosa mesenchymal stem cells (OM-MSCs) have the ability to promote regeneration in the nervous system in vivo. Moreover, with view to the potential for clinical application, OM-MSCs have the advantage of being easily accessible from patients and transplantable in an autologous manner, thus eliminating immune rejection and contentious ethical issues. So far, most studies have been focused on the role of OM-MSCs in central nervous system replacement. However, the secreted proteomics of OM-MSCs have not been reported yet. Here, proteins secreted by OM-MSCs cultured in serum-free conditions were separated on SDS-PAGE and identified by LC-MS/MS. As a result, a total of 274 secreted proteins were identified. These molecules are known to be important in neurotrophy, angiogenesis, cell growth, differentiation, and apoptosis, and inflammation which were highly correlated with the repair of central nervous system. The proteomic profiling of the OM-MSCs secretome might provide new insights into their nature in the neural recovery. However, proteomic analysis for clinical biomarkers of OM-MSCs needs to be further studied.

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

“…They also express p75-NTR and SMA [77]. Consistent with the characteristics of multilineage diferentiation capacity of MSCs, OM-MSCs can be diferentiated into osteocytes and adipocytes [13,82,88], chondrocytes [78,90], neuron like cells [13,78,81] and myocardial-like cells [80] when cultured under appropriate conditions. They, therefore, present abilities to diferentiate into mesodermal and ectodermal cell types.…”

“…MSCs can be obtained from a vast array of tissues that include adipose tissue, lungs, bone marrow, umbilical cord (Wharton's jelly and umbilical cord blood), synovium, amniotic luid, fetal blood, dental pulp, skeletal muscle, circulatory system [68,69] and olfactory mucosa [13]. Applied to regenerative medicine, MSCs present exceptional features that make them great options, such as easy expansion, diferentiation into diferent cell types, immune-privileges and immune modulation, tropism to injured sites, trophic stimulation and modulation of tissues functions [46].…”

“…Phenotypic analysis reveals the presence of Stro-1 [75][76][77], CD29 [80,88], CD44 [80,88], CD49b [90], CD54 [13,77,82], CD73 [77,82,90], CD90 [13, 75-78, 80, 82, 88, 90], CD105 [13, 75-78, 80, 82], CD106 [77,78] and CD166 [77,78,80,82,90] markers and the absence of haematopoietic stem cell markers such as CD34 [13, 75-78, 80, 88, 90], CD45 [13, 75-78, 80, 88] and CD11b [88]. Strangely, one work indicates lack of Stro-1 [82].…”

“…The only study of the determination of the secretome and metabolome of OM-MSCs allowed to identify 274 proteins in OM-MSCs conditioned medium and the identiication of some processes that are usually associated to transplantation processes such as biological regulation, cellular processes, metabolic processes, development processes and response to stimuli [13]. These processes promote repairing by facilitating migration to injured sites, remodelling the extracellular matrix and increasing metabolism and cellular activity.…”

Standardizing Harvesting Methods for Human Turbinate Mesenchymal Stem Cells from Surgical Tissue

“…Our study may help address this issue as by using hTMSCs for reprogramming, it is possible to get the unique characteristics of neuronal cells and solve the problem of nerve regeneration. This may be due to the fact that olfactory cells secrete unique materials that promote neural differentiation [4].…”