Secretome analysis of human bone marrow derived mesenchymal stromal cells

Baberg, Falk; Geyh, Stefanie; Waldera-Lupa, Daniel M.; Stefanski, Anja; Zilkens, Christoph; Haas, Rainer; Schroeder, Thomas; Stühler, Kai

doi:10.1016/j.bbapap.2019.01.013

Cited by 43 publications

(28 citation statements)

References 50 publications

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“…In a recent approach, we described the comparison of abundances of secreted and cellular proteins as a valuable tool to select proteins that are enriched in the secretome and therefore probably secreted 15 . Using this approach in ten different experimental settings, including mouse, rat, and human cell types 16,17 , we developed a database containing proteins showing a high likelihood to be secreted in the respective systems (called secreted proteins in this chapter) as well as those showing very high likelihood to not be secreted (called cellular proteins in this chapter). The proteins that were secreted in at least three different experiments and did not contain a signal peptide and/or transmembrane domain (UniProt Annotation release May 2018) were selected as the training set for OutCyte-UPS.…”

Section: Methodsmentioning

confidence: 99%

OutCyte: a novel tool for predicting unconventional protein secretion

Zhao

Poschmann

Waldera-Lupa

et al. 2019

Sci Rep

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The prediction of protein localization, such as in the extracellular space, from high-throughput data is essential for functional downstream inference. It is well accepted that some secreted proteins go through the classic endoplasmic reticulum-Golgi pathway with the guidance of a signal peptide. However, a large number of proteins have been found to reach the extracellular space by following unconventional secretory pathways. There remains a demand for reliable prediction of unconventional protein secretion (UPS). Here, we present OutCyte, a fast and accurate tool for the prediction of UPS, which for the first time has been built upon experimentally determined UPS proteins. OutCyte mediates the prediction of protein secretion in two steps: first, proteins with N-terminal signals are accurately filtered out; second, proteins without N-terminal signals are classified as UPS or intracellular proteins based on physicochemical features directly generated from their amino acid sequences. We are convinced that OutCyte will play a relevant role in the annotation of experimental data and will therefore contribute to further characterization of the extracellular nature of proteins by considering the commonly neglected UPS proteins.OutCyte has been implemented as a web server at www.outcyte.com.

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Section: Methodsmentioning

confidence: 99%

OutCyte: a novel tool for predicting unconventional protein secretion

Zhao

Poschmann

Waldera-Lupa

et al. 2019

Sci Rep

Self Cite

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“…Several mechanisms may contribute to tendon repair with exogenic BMSCs. First, BMSCs can differentiate into certain new cells (tenocytes) to replace lost normal cells[ 19 , 39 ]; second, BMSCs can secrete various cytokines and growth factors to promote the proliferation of cells in injured tissue[ 40 ]; and third, BMSCs can increase the deposition of collagenous proteins[ 41 ]. BMSC-based therapy has been found to improve histological and biomechanical properties and to increase the expression of collagen in animal injury[ 42 , 43 ].…”

Section: Endogenous and Exogenous Stem Cells/progenitors For Tendon Rmentioning

confidence: 99%

Mechanotransduction of stem cells for tendon repair

Wang

Huang

2020

WJSC

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Tendon is a mechanosensitive tissue that transmits force from muscle to bone. Physiological loading contributes to maintaining the homeostasis and adaptation of tendon, but aberrant loading may lead to injury or failed repair. It is shown that stem cells respond to mechanical loading and play an essential role in both acute and chronic injuries, as well as in tendon repair. In the process of mechanotransduction, mechanical loading is detected by mechanosensors that regulate cell differentiation and proliferation via several signaling pathways. In order to better understand the stem-cell response to mechanical stimulation and the potential mechanism of the tendon repair process, in this review, we summarize the source and role of endogenous and exogenous stem cells active in tendon repair, describe the mechanical response of stem cells, and finally, highlight the mechanotransduction process and underlying signaling pathways.

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“…Pre-conditioning tendon cells by in-vitro culture with BM-MSCs secretome and further combining those cells with an electrospun keratin-based scaffold resulted in improved biomechanical performance in an in-vivo rat model of chronic massive rotator cuff tear [82]. These outcomes suggest a beneficial effect of BM-MSCs secretome, which has been demonstrated to include several growth factors, cytokines and other soluble molecules intervening in cellular growth and/or maintenance and signal transduction processes [83].…”

Section: Cell-based Therapies For Tendon Applicationsmentioning

confidence: 99%

Mesenchymal Stem Cells Empowering Tendon Regenerative Therapies

Costa‐Almeida

Calejo

Gomes

2019

IJMS

103

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Tendon tissues have limited healing capacity. The incidence of tendon injuries and the unsatisfactory functional outcomes of tendon repair are driving the search for alternative therapeutic approaches envisioning tendon regeneration. Cellular therapies aim at delivering adequate, regeneration-competent cell types to the injured tendon and toward ultimately promoting its reconstruction and recovery of functionality. Mesenchymal stem cells (MSCs) either obtained from tendons or from non-tendon sources, like bone marrow (BM-MSCs) or adipose tissue (ASCs), have been receiving increasing attention over the years toward enhancing tendon healing. Evidences from in vitro and in vivo studies suggest MSCs can contribute to accelerate and improve the quality of tendon healing. Nonetheless, the exact mechanisms underlying these repair events are yet to be fully elucidated. This review provides an overview of the main challenges in the field of cell-based regenerative therapies, discussing the role of MSCs in boosting tendon regeneration, particularly through their capacity to enhance the tenogenic properties of tendon resident cells.

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Secretome analysis of human bone marrow derived mesenchymal stromal cells

Cited by 43 publications

References 50 publications

OutCyte: a novel tool for predicting unconventional protein secretion

OutCyte: a novel tool for predicting unconventional protein secretion

Mechanotransduction of stem cells for tendon repair

Mesenchymal Stem Cells Empowering Tendon Regenerative Therapies

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