Secondary Metabolites of a Mangrove Endophytic Fungus Aspergillus terreus (No. GX7-3B) from the South China Sea

Abstract

The mangrove endophytic fungus Aspergillus terreus (No. GX7-3B) was cultivated in potato dextrose liquid medium, and one rare thiophene compound (1), together with anhydrojavanicin (2), 8-O-methylbostrycoidin (3), 8-O-methyljavanicin (4), botryosphaerone D (5), 6-ethyl-5-hydroxy-3,7-dimethoxynaphthoquinone (6), 3β,5α-dihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (7), 3β,5α,14α-trihydroxy-(22E,24R)-ergosta-7,22-dien-6-one (8), NGA0187 (9) and beauvericin (10), were isolated. Their structures were elucidated by analysis of spectroscopic data. This is the first report of a natural origin for compound 6. Moreover, compounds 3, 4, 5, 7, 8 and 10 were obtained from marine microorganism for the first time. In the bioactive assays in vitro, compounds 2, 3, 9 and 10 displayed remarkable inhibiting actions against α-acetylcholinesterase (AChE) with IC50 values 2.01, 6.71, 1.89, and 3.09 μM, respectively. Furthermore, in the cytotoxicity assays, compounds 7 and 10 exhibited strong or moderate cytotoxic activities against MCF-7, A549, Hela and KB cell lines with IC50 values 4.98 and 2.02 (MCF-7), 1.95 and 0.82 (A549), 0.68 and 1.14 (Hela), and 1.50 and 1.10 μM (KB), respectively; compound 8 had weak inhibitory activities against these tumor cell lines; compounds 1, 2, 3, 4, 5, 6 and 9 exhibited no inhibitory activities against them.

Isochromanone derivatives from the endophytic fungus Fusarium sp. PDB51F5

“…One new isochromenone, fusaraisochromenone (1), and one new isochromanone, fusaraisochromanone (2) (Fig. 1), were isolated from the broth extract (ethyl acetate) together with 11 known compounds; (R)-3,4-dihydro-4,8-dihydroxy-6-methoxy-4,5-dimethyl-3-methyleneisochromen-1-one (3), 4 8-O-methyljavanicin (4), 5 3-acetyl-7-hydroxy-5-methoxyl-3H-isobenzofuran-l-one (5), 6 curvulin (6), 7 fusalanipyrone (7), 8 daidzein (8), 9 formononetin (9), 9 7-O-methyl genistein (10), 10 kakkatin (11), 10 p-hydroxy benzoic acid (12), 11 and tyrosol (13). 12 All of the compounds (1-13) were isolated using chromatographic techniques (see ESI) and their structures elucidated from spectroscopic data (IR, UV, 1D, and 2D NMR and by mass spectrometry).…”

Metabolites from Two Dominant Thermophilic Fungal Species Thermomyces lanuginosus and Scytalidium thermophilum

“…tritici , Phytophthora capisici , and Rhizoctonia cerealis . In 2013, 16 was identified from a mangrove endophytic fungus Aspergillus terreus and found to have weak inhibitory activities against human breast cancer cells (MCF‐7), lung cancer cells (A549), cervix carcinoma cells (HeLa) and human nasopharyngeal carcinoma cells (KB) …”

Fusarihexins A and B: Novel Cyclic Hexadepsipeptides from the Mangrove Endophytic Fungus Fusarium sp. R5 with Antifungal Activities

“…Compounds 1 and 2 exhibited potent antifungal activities against the plant pathogens C. gloeosporioides and C. musae, and compounds 1 and 3 towards F. oxysporum, yielding better results compared with the positive control carbendazim, thus indicating that these compounds could be potential antifungal agents. To date, cyclic hexadepsipeptides have been reported to possess diverse bioactivities, including antimalaria, antituberculosis, cytotoxic [28], acetylcholinesterase inhibitory [29], antitrypanosomal [30], anti-inflammatory [31], insecticidal [32], antibacterial [33], phtotoxic [34], and immunosuppressive [21] activities. However, only a few cyclic hexadepsipeptides have been reported to possess antifungal activities.…”