Secondary metabolite profiling, cytotoxicity, anti-inflammatory potential and in vitro inhibitory activities of Nardostachys jatamansi on key enzymes linked to hyperglycemia, hypertension and cognitive disorders

Bose, Biswajit; Tripathy, Debabrata; Chatterjee, Anupam; Tandon, Pramod; Kumaria, Suman

doi:10.1016/j.phymed.2018.08.010

Cited by 38 publications

(20 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, metabolomics-based approaches have become increasingly applied to all aspects of diabetes research. Recent literature shows that metabolomics has been applied to biomarker and drug discovery, drug assessment [259, 260], toxicology [261, 262], and clinical biochemistry with a particular focus on gestational diabetes mellitus (GDM) [263–270]. Some of the recent metabolomics-aided discoveries in antidiabetic plant research are summarised below.…”

Section: Metabolite Profilingmentioning

confidence: 99%

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

2019

View full text Add to dashboard Cite

Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitations, the alarming increase in the prevalence of diabetes, and the soaring cost of managing diabetes and its complications underscores an urgent need for safer, more efficient and affordable alternative treatments. Over 1200 plant species are reported in ethnomedicine for treating diabetes and these represents an important and promising source for the identification of novel antidiabetic compounds. Evaluating medicinal plants for desirable bioactivity goes hand-in-hand with methods in analytical biochemistry for separating and identifying lead compounds. This review aims to provide a comprehensive summary of current methods used in antidiabetic plant research to form a useful resource for researchers beginning in the field. The review summarises the current understanding of blood glucose regulation and the general mechanisms of action of current antidiabetic medications, and combines knowledge on common experimental approaches for screening plant extracts for antidiabetic activity and currently available analytical methods and technologies for the separation and identification of bioactive natural products. Common in vivo animal models, in vitro models, in silico methods and biochemical assays used for testing the antidiabetic effects of plants are discussed with a particular emphasis on in vitro methods such as cell-based bioassays for screening insulin secretagogues and insulinomimetics. Enzyme inhibition assays and molecular docking are also highlighted. The role of metabolomics, metabolite profiling, and dereplication of data for the high-throughput discovery of novel antidiabetic agents is reviewed. Finally, this review also summarises sample preparation techniques such as liquid–liquid extraction, solid phase extraction, and supercritical fluid extraction, and the critical function of nuclear magnetic resonance and high resolution liquid chromatography–mass spectrometry for the dereplication, putative identification and structure elucidation of natural compounds from evidence-based medicinal plants.

show abstract

Section: Metabolite Profilingmentioning

confidence: 99%

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

2019

View full text Add to dashboard Cite

show abstract

“…Overall, our findings revealed quite good correlations between phenolics and biological properties. In this regard, secondary metabolites (such as polyphenols) are known to be characterized by different mechanisms of radical scavenging and enzymatic inhibitions, thus making them potential agents to treat many ailments, such as cardiovascular diseases, cancer, diabetes and other pathologies [ 36 , 37 , 38 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia

et al. 2021

View full text Add to dashboard Cite

Lupin seeds can represent a valuable source of phenolics and other antioxidant compounds. In this work, a comprehensive analysis of the phytochemical profile was performed on seeds from three Lupinus species, including one cultivar (Lupinus albus) and two wild accessions (Lupinus cossentinii and Lupinus luteus), collected from the northern region of Tunisia. Untargeted metabolomic profiling allowed to identify 249 compounds, with a great abundance of phenolics and alkaloids. In this regard, the species L. cossentinii showed the highest phenolic content, being 6.54 mg/g DW, followed by L. luteus (1.60 mg/g DW) and L. albus (1.14 mg/g DW). The in vitro antioxidant capacity measured by the ABTS assay on seed extracts ranged from 4.67 to 17.58 mg trolox equivalents (TE)/g, recording the highest values for L. albus and the lowest for L. luteus. The DPPH radical scavenging activity ranged from 0.39 to 3.50 mg TE/g. FRAP values varied between 4.11 and 5.75 mg TE/g. CUPRAC values for lupin seeds ranged from 7.20 to 8.95 mg TE/g, recording the highest for L. cossentinii. The results of phosphomolybdenum assay and metal chelation showed similarity between the three species of Lupinus. The acetylcholinesterase (AChE) inhibition activity was detected in each methanolic extract analyzed with similar results. Regarding the butyrylcholinesterase (BChE) enzyme, it was weakly inhibited by the Lupinus extracts; in particular, the highest activity values were recorded for L. albus (1.74 mg GALAE/g). Overall, our results showed that L. cossentinii was the most abundant source of polyphenols, consisting mainly in tyrosol equivalents (5.82 mg/g DW). Finally, significant correlations were outlined between the phenolic compounds and the in vitro biological activity measured, particularly when considering flavones, phenolic acids and lower-molecular-weight phenolics.

show abstract

“…Corroborating this hypothesis, researchers reported that a plasma increase of BChE is correlated with IL-6 and C-reactive protein in patients with a stroke (Kliper et al, 2013). Increases in BChE activity have been identified in pathologies, such as Alzheimer's disease (Geula and Darvesh, 2004), diabetes mellitus (Rao et al, 2007), obesity (Dantas et al, 2011) and hypertension (Bose et al, 2019). In this context, plasma cholinergic signaling may contribute to the induction or maintenance of a low-grade inflammatory state in metalworkers, making them susceptible to the development of several chronic pathologies (Hopf et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Accelerated apoptosis, oxidative stress, and cholinergic inflammation in blood of metalworkers

et al. 2021

View full text Add to dashboard Cite

Metalworkers are exposed to numerous chemicals in their workplace environment, such as solvents, heavy metals, and metalworking fluids, that have a negative impact on their health. Furthermore, there is an increase in the prevalence of chronic diseases among metalworkers; however, the molecular mechanisms involved in this increased predisposition to chronic diseases are unclear. Considering that occupational exposure represents a potential risk for metalworkers, the aim of this study was to measure biomarkers of oxidative stress, inflammation, and cytotoxicity in the peripheral blood of metalworkers from Southern Brazil. The study included 40 metalworkers and 20 individuals who did not perform activities with any recognized exposure to chemical substances, such as those working in administration, commerce, and education, as controls. Cellular and molecular biomarkers as leukocyte viability, intracellular production of reactive species, mitochondrial mass and membrane potential and plasma lipid peroxidation, sulfhydryl groups, total antioxidant capacity, and butyrylcholinesterase activity were evaluated in the blood of metalworkers and controls. Metalworkers were found to have higher rates of apoptosis, increased production of reactive species, and increased mitochondrial potential and mass in leukocytes associated with decreased antioxidant defenses and increased activity of the butyrylcholinesterase enzyme in their plasma. It can be concluded that cytotoxicity, oxidative stress, and inflammation are involved in the multiplicity of health outcomes related to chemical exposure in the metalworking industry.

show abstract

Secondary metabolite profiling, cytotoxicity, anti-inflammatory potential and in vitro inhibitory activities of Nardostachys jatamansi on key enzymes linked to hyperglycemia, hypertension and cognitive disorders

Cited by 38 publications

References 87 publications

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

Understanding glycaemic control and current approaches for screening antidiabetic natural products from evidence-based medicinal plants

Untargeted Phytochemical Profile, Antioxidant Capacity and Enzyme Inhibitory Activity of Cultivated and Wild Lupin Seeds from Tunisia

Accelerated apoptosis, oxidative stress, and cholinergic inflammation in blood of metalworkers

Contact Info

Product

Resources

About