Secondary metabolite profiles and antifungal drug susceptibility of Aspergillus fumigatus and closely related species, Aspergillus lentulus, Aspergillus udagawae, and Aspergillus viridinutans

Tamiya, Hiroyuki; Ochiai, Eri; Kikuchi, Kazuyo; Yahiro, Maki; Toyotome, Takahito; Watanabe, Akira; Yaguchi, Takashi; Kamei, Katsuhiko

doi:10.1016/j.jiac.2015.01.005

Cited by 42 publications

(43 citation statements)

References 44 publications

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“…Perhaps the differences are influenced by that fact that A. fumigatus Af293 is from a clinical isolate, whereas A. novofumigatus have been isolated from chamise chaparral soil after a bush fire in Southern California (2,24). Indeed, earlier analyses have shown that clinical isolates produce fewer exometabolites and sporulate less (4,30,31).…”

Section: Secondary Metabolite Profile Of a Novofumigatus Compared Withmentioning

confidence: 99%

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Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species

Kjærbølling

Vesth

Frisvad

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

120

116

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The fungal genus of is highly interesting, containing everything from industrial cell factories, model organisms, and human pathogens. In particular, this group has a prolific production of bioactive secondary metabolites (SMs). In this work, four diverse species (, ,, and )have been whole-genome PacBio sequenced to provide genetic references in three sections. and also were sequenced for SM elucidation. Thirteen genomes were analyzed with comparative genomics to determine phylogeny and genetic diversity, showing that each presented genome contains 15-27% genes not found in other sequenced Aspergilli. In particular, was compared with the pathogenic species This suggests that can produce most of the same allergens, virulence, and pathogenicity factors as, suggesting that could be as pathogenic as Furthermore, SMs were linked to gene clusters based on biological and chemical knowledge and analysis, genome sequences, and predictive algorithms. We thus identify putative SM clusters for aflatoxin, chlorflavonin, and ochrindol in ,, and , respectively, and novofumigatonin,-cycloechinulin, and -aszonalenins in Our study delivers six fungal genomes, showing the large diversity found in the genus; highlights the potential for discovery of beneficial or harmful SMs; and supports reports of pathogenicity. It also shows how biological, biochemical, and genomic information can be combined to identify genes involved in the biosynthesis of specific SMs.

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Section: Secondary Metabolite Profile Of a Novofumigatus Compared Withmentioning

confidence: 99%

“…It is likely that different combinations of virulence factors among the species affect pathogenicity (31). It has been suggested that species unable to produce some metabolites may be able to produce proxy-exometabolites that can serve the same function.…”

Section: Secondary Metabolite Profile Of a Novofumigatus Compared Withmentioning

confidence: 99%

Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species

Kjærbølling

Vesth

Frisvad

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

120

116

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“…Species in Aspergillus section Fumigati are both phenotypically and genotypically distinct (Raper and Fennell, 1965 ; Geiser et al, 1998 ; Hong et al, 2005 , 2006 , 2008 ; Katz et al, 2005 ; Geiser et al, 2007 ; Samson et al, 2007 ; Yaguchi et al, 2007 ). Aspergillus lentulus was originally claimed to be a sibling species of A. fumigatus , but was later shown to be phenotypically very different from A. fumigatus , especially concerning extrolite profiles (Larsen et al, 2007 ; Tamiya et al, 2015 ). The species A. pseudofelis, A. parafelis , and A. pseudoviridinutans have not been examined chemically, but they are very close phylogenetically and morphologically to A. felis and may be real sibling species with no phenotypic differences (Sugui et al, 2014 ).…”

Section: Aspergillus Taxonomymentioning

confidence: 99%

Extrolites of Aspergillus fumigatus and Other Pathogenic Species in Aspergillus Section Fumigati

Frisvad

Larsen

2016

Front. Microbiol.

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Aspergillus fumigatus is an important opportunistic human pathogen known for its production of a large array of extrolites. Up to 63 species have been described in Aspergillus section Fumigati, some of which have also been reliably reported to be pathogenic, including A. felis, A. fischeri, A. fumigatiaffinis, A. fumisynnematus, A. hiratsukae, A. laciniosus, A. lentulus, A. novofumigatus, A. parafelis, A. pseudofelis, A. pseudoviridinutans, A. spinosus, A. thermomutatus, and A. udagawae. These species share the production of hydrophobins, melanins, and siderophores and ability to grow well at 37°C, but they only share some small molecule extrolites, that could be important factors in pathogenicity. According to the literature gliotoxin and other exometabolites can be contributing factors to pathogenicity, but these exometabolites are apparently not produced by all pathogenic species. It is our hypothesis that species unable to produce some of these metabolites can produce proxy-exometabolites that may serve the same function. We tabulate all exometabolites reported from species in Aspergillus section Fumigati and by comparing the profile of those extrolites, suggest that those producing many different kinds of exometabolites are potential opportunistic pathogens. The exometabolite data also suggest that the profile of exometabolites are highly specific and can be used for identification of these closely related species.

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“…Pyrenocine J showed cytotoxic activity against the human hepatic cancer cell line HepG2 with an IC (50) value of 28.5 μg/ml. The presence of different mycotoxins, gliotoxin, sphingofungins, subroutines, and verruculogen in Aspergillus fumigatus a close relative of A. neoflavipes has also been reported (Tamiya et al, 2015) which are of great commercial value. The capacity of producing Vinblastin, an anticancerous drug from Alternaria sp isolated from C. roseus was reported (Guo et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…However literature reveals that Curvularia affinis, Curvularia fallax, and Curvularia senegalensis are synonymous to C. geniculata. The presence of the mycotoxins, fumifungin, fumiquinazoline A/B and D, fumitremorgin B, gliotoxin, sphingofungins, subroutines, and verruculogen in Aspergillus fumigatus a close relative of A. neoflavipes has been reported (Tamiya et al, 2015). The presence of isochromenone, from an endophytic fungus Aspergillus fumigatus isolated from Bacopa monnieri plant and proof its antioxidant and antitubercular activity has been investigated (Thakur et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogeny and genetic diversity studies of some potent endophytic fungi isolated from medicinal plants (Calotropis procera and Catharanthus roseus) using 18S rRNA and RAPD analysis

Chowdhury¹,

Chattopadhyay²,

Roy³

2018

Afr. J. Microbiol. Res.

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Molecular identification of four endophytic fungi (Penicillium singorense, Curvularia geniculata, Aspergillus neoflavipes and Alternaria alternata) from two important medicinal plants, Calotropis procera (L.) R.Br. and Catharanthus roseus (L.) G.Don. was carried out by 18SrRNA sequencing. Genetic diversity using two RAPD primer viz. OPB-07 and OPC-06 showed good result with high polymorphism between the four strains. The sample DRC1 Accession No. MG322179 indicated strong homology with Penicillium singorense, strain DI16-118 (Accession No. LT558940), sample DRC2 Accession No. MG322180 with Curvularia geniculata strain F1 (Accession No. KX022497), sample DRC3 Accession No. MG322181 with Aspergillus neoflavipes strain AJR1 ribosomal gene (Accession No. KX218387), sample DRC4 Accession No. MH021686 with A. alternata strain AE1 18SrRNA gene, partial sequence, ITS1, 5.8S rRNA gene and ITS 2 complete sequence (Accession No. KY676196). Phylogenetic tree showing relatedness of sample DRC1 Accession No. MG322179 with 19 strains of Penicillium sp, DRC2 Accession No. MG322180 with 34 strains of Curvularia sp, DRC3 Accession No. MG322181 with 24 strains of Aspergillus sp and DRC4 Accession No. MH021686 with 16 strains of Alternaria sp of diverse origin. The present investigation thus gave an insight for the detection and genetic diversity study among the fungal endophytes isolated from two important Indian medicinal plants (C. procera and C. roseus) and it would probably the first report of its own kind from West Bengal, India.

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Secondary metabolite profiles and antifungal drug susceptibility of Aspergillus fumigatus and closely related species, Aspergillus lentulus, Aspergillus udagawae, and Aspergillus viridinutans

Cited by 42 publications

References 44 publications

Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species

Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species

Extrolites of Aspergillus fumigatus and Other Pathogenic Species in Aspergillus Section Fumigati

Molecular phylogeny and genetic diversity studies of some potent endophytic fungi isolated from medicinal plants (Calotropis procera and Catharanthus roseus) using 18S rRNA and RAPD analysis

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