Sebum: analysis by infrared spectroscopy. II. The suppression of fatty acids by systemically administered antibiotics

Pablo, Gary

doi:10.1001/archderm.111.6.734

Cited by 6 publications

(6 citation statements)

References 6 publications

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“…pilosebaceous follicles and acne lesions (20), are closely associated with acne vulgaris. Broadspectrum antibiotics used in acne therapy are thought to decrease the numbers of viable P. acnes recovered from lesions and hence reduce the amount of free fatty acids in the sebum (2, 7,8,18). In these studies, however, bacteriological sampling methods have been used which do not necessarily represent the organisms of the pilosebaceous duct.…”

Section: Discussionmentioning

confidence: 99%

“…Improvement in acne is often achieved by long-term administration of broad-spectrum antibiotics which are thought to decrease the density of propionibacteria (1, 12,14) and hence reduce the free fatty acids in the sebum (7,18). At present, there are few reports describing the effects of antibiotics on propionibacteria in vitro, those that do exist being mainly concerned with the evaluation of antimicrobial susceptibility (9,19).…”

mentioning

confidence: 99%

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Effect of clindamycin, erythromycin, lincomycin, and tetracycline on growth and extracellular lipase production by propionibacteria in vitro

Unkles¹,

Gemmell²

1982

Antimicrob Agents Chemother

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Two propionibacteria identified as Propionibacterium acnes and Propionibacterium granulosum were grown anaerobically in the presence of growth subinhibitory concentrations (0.25 and 0.5 minimal inhibitory concentrations) of clindamycin, erythromycin, lincomycin, and tetracycline. Viable counts and assays of extracellular lipase were performed on samples taken at 24-h intervals over a 96-h period. The results showed that lincomycin and clindamycin could inhibit the production of the enzyme by both strains with little effect on their growth rates. Tetracycline caused inhibition of lipase production by P. granulosum only. Although production of the enzyme by P. acnes was delayed in the presence of tetracycline, the final titer was the same as the control. Erythromycin had little effect on growth and enzyme production of either strain. It is possible, therefore, that certain antibiotics used in acne therapy may act not only as bactericidal agents but also as inhibitors of enzyme production under non-growth-limiting conditions. Hydrolysis of sebum triglycerides by bacterial lipases, in particular those of propionibacteria (13,20), and the subsequent release of irritating free fatty acids in the pilosebaceous follicles has been proposed as a major factor in the formation of lesions in acne vulgaris (22). Improvement in acne is often achieved by long-term administration of broad-spectrum antibiotics which are thought to decrease the density of propionibacteria (1,12,14) and hence reduce the free fatty acids in the sebum (7, 18). At present, there are few reports describing the effects of antibiotics on propionibacteria in vitro, those that do exist being mainly concerned with the evaluation of antimicrobial susceptibility (9,19). In a recent publication, however, Webster et al. (26) found that lipase production in vitro by a strain of Propionibacterium acnes was inhibited by subgrowth-inhibitory concentrations of tetracycline and declomycin and delayed by erythromycin. The present study sought, therefore, to investigate the effect of antibiotics, commonly used in acne therapy, at subinhibitory concentrations on the growth and extracellular lipase production of P. acnes and Propionibacterium granulosum. Levels of drug at one-half and one-fourth minimal inhibitory concentrations (MIC) were chosen for this study. A similar experimental system has been used to follow the biosynthesis of toxins and enzymes in Staphylococcus aureus (5) and Streptococcus pyogenes (4). MATERIALS AND METHODSBacteral strains. Two strains (P2 and P27) were selected from a collection of 52 Propionibacterium strains which had been isolated from normal skin and maintained anaerobically on Brewer's agar without indicator (Difco Laboratories, Detroit, Mich.), supplemented with 1% Tween 80 (Koch Light, Ltd., Colnbrook, England). The strains were identified using the biochemical classification scheme of Marples and McGinley (15): strain P2 was classified as P. acnes, and strain P27 was classified as P. granulosum.Antibiotics. The antibiotics used in thi...

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Effect of clindamycin, erythromycin, lincomycin, and tetracycline on growth and extracellular lipase production by propionibacteria in vitro

Unkles¹,

Gemmell²

1982

Antimicrob Agents Chemother

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“…Variations in sebum production and intake of drugs (e.g. retinoids, antibiotics) are likely associated with subtle changes in its molecular composition [12] which in turn may alter the cornification of the infundibu- lum and be a key feature for comedogenesis [13,14]. Other changes in the composition of the sebum have been ascribed to specific diseases such as AIDS [15].…”

Section: Micro-anatomy and Function Of Sebaceous Glandsmentioning

confidence: 99%

EEMCO Guidance for the in vivo Assessment of Skin Greasiness

Pierard

Piérard-Franchimont

Marks

et al. 2000

Skin Pharmacol Physiol

140

119

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Sebaceous gland activity has four distinct components which are sebum production (a secretion rate function), storage (a volume function), surface output (a delivery rate function) and stratum corneum permeation (an influx rate function). The oily appearance of skin results from an excess of sebum excretion and spreading over the body surface and its interaction with the skin surface. A multi-pronged approach is often useful to assess skin greasiness with precision. The clinical evaluation of skin greasiness and its shiny appearance should be further complemented by quantifying the large pores, follicular plugs and comedones. The sebum amount present at the skin surface can be measured non-invasively using one of several methods based on solvent extraction, cigarette paper pads, photometric assessment, bentonite clay and lipid-sensitive tapes. Quantitative parameters include the sebum casual level, the sebum excretion rate, the sebum replacement time, the instant sebum delivery, the follicular excretion rate, the density in sebum-enriched reservoirs and the sustainable rate of sebum excretion. A series of environmental and biological features influence the data. Hence rigorous methodological designs are mandatory to support claims. As a rule, accuracy of the methods is adversely affected by skin temperature, degree of hydration and surface roughness. An additional confounding factor is the inherent difficulty of collecting the surface lipids without a contribution from the follicular reservoir. A better understanding of factors that alter the sebum amount at the skin surface may well assist in the development of sebosuppressive agents to help the reduction of the skin greasiness and improve acne.

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“…Trabalhos científicos demonstram que alguns antibióticos têm a capacidade de alterar a composição do SB de pacientes portadores de AV; as tetraciclinas são mais efetivas que as eritromicinas (a clindamicina é a mais potente desta classe) neste objetivo (Pablo;Fulton Jr., 1975). Mostra-se uma redução nas quantidades de AGL em prol de um aumento dos TG (Feinkel et al, 1965;Powell, 1969).…”

Section: Ou III Em Uso Sistêmico De Limeciclina E/ou Suplementaçãunclassified

“…Observa-se que as tetraciclinas são mais efetivas que as eritromicinas (sendo a clindamicina a mais potente desta classe) (Pablo;Fulton Jr, 1975 …”

Section: Sebo E Antibioticoterapia Sistêmicaunclassified

Estudo das mudanças quali e quantitativas da participação de ácidos graxos no sebo de pacientes com acne inflamatória, graus II ou III, em uso sistêmico de limeciclina e/ou suplementação oral à base de ácidos linoleico, gamalinolênico

Costa¹

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Sebum: analysis by infrared spectroscopy. II. The suppression of fatty acids by systemically administered antibiotics

Cited by 6 publications

References 6 publications

Effect of clindamycin, erythromycin, lincomycin, and tetracycline on growth and extracellular lipase production by propionibacteria in vitro

Effect of clindamycin, erythromycin, lincomycin, and tetracycline on growth and extracellular lipase production by propionibacteria in vitro

EEMCO Guidance for the in vivo Assessment of Skin Greasiness

Estudo das mudanças quali e quantitativas da participação de ácidos graxos no sebo de pacientes com acne inflamatória, graus II ou III, em uso sistêmico de limeciclina e/ou suplementação oral à base de ácidos linoleico, gamalinolênico

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