Seasonal variation of serum lysozyme levels in Atlantic halibut (Hippoglossus hippoglossus L.)

Bowden, Timothy J.; Bütler, R; Bricknell, Ian

doi:10.1016/j.fsi.2003.12.001

Cited by 49 publications

(28 citation statements)

References 20 publications

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“…Lysozyme activity was measured using the turbidimetric assay as described previously [14]. Briefly, 50 µL of serum was pipetted, in duplicate, in a 96-well plate (Iwaki, Tokyo, Japan).…”

Section: Lysozyme Assaymentioning

confidence: 99%

Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>)

Le¹,

Fotedar²

2013

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The dietary selenium (Se) requirement of yellowtail kingfish (Seriola lalandi) in normal and infected conditions was investigated. The fish were fed one of five experimental diets; a control un-supplemented diet (3.35 mg/kg Se) or diets supplemented with Se to provide 4.86, 5.38, 5.85 or 6.38 mg/kg Se. After feeding for 6 weeks, the fish were challenged by Vibrio anguillarum immersion and then observed for 4 weeks. Supplementation of Se had no effect on feed intake, feed conversion ratio and survival over 6 weeks of feeding; however, it significantly increased growth and Se content in muscle tissues. The optimal Se level for maximal growth of yellowtail kingfish estimated by second order regression was 5.56 mg/kg. Following the bacterial challenge, the immune-stimulating effects of Se were demonstrated in lysozyme and bactericidal activities, and there was a corresponding increase in survival and antibody response by supplementation of Se at ≥2 mg/kg (measured Se of ≥5.38 mg/kg). Under normal and infectious conditions, antioxidant capacity of fish measured as glutathione peroxidase activity increased by supplementation of Se. During post-challenge period, haematocrits were higher in the fish fed Se supplemented diets than the fish fed the control diet, while more macrophage aggregates were seen in the control group than in the others. Furthermore, there was evidence of myopathy in fish fed the diet without Se supplementation. Therefore, the results indicated that the optimal dietary Se requirement of yellowtail kingfish is 5.56 mg/kg.

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“…Lysozyme activity was measured using the turbidimetric assay as described previously [14]. Briefly, 50 µL of serum was pipetted, in duplicate, in a 96-well plate (Iwaki, Tokyo, Japan).…”

Section: Lysozyme Assaymentioning

confidence: 99%

Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>)

Le¹,

Fotedar²

2013

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“…Lysozyme acts as an acute-phase protein, being released not only in response to bacterial antigens but also to other alarm situations such as stress (Demers & Bayne 1997). Consequently, the level or activity of this enzyme has been widely used as an index to evaluate fish defensive capacity, towards disease and/or any other challenging situation (Bowden et al 2004, Wu et al 2007, Costas et al 2011. The nitroblue tetrazolium (NBT) assay is mostly used to measure the oxidative radical production by leukocytes in defence against pathogens (Jeney & Anderson 1993, Jeney et al 1997, Castro et al 1999, Cook et al 2003, Sahoo et al 2005.…”

Section: Sarcoma In the Thymus Of Juvenile Meagrementioning

confidence: 99%

Sarcoma in the thymus of juvenile meagre Argyrosomus regius reared in an intensive system

Soares¹,

Leitão²,

Moreira³

et al. 2012

Dis. Aquat. Org.

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Juvenile meagre Argyrosomus regius (Asso, 1809) maintained in experimental conditions developed lateral and/or bilateral circular-shaped sarcoma within the opercular cavity. The sarcoma was dense, reddish and its growth from the branchial arch exerted pressure on the operculum forcing it to open. Histologically, the neoplasm exhibited marked proliferation of mesenchymal connective tissue composed largely of fusiform cells, which developed in a solid pattern accompanied by abundant mononuclear cell types. Multifocal areas of discrete necrosis were also observed, compatible with a sarcomatous proliferation. The immunological parameters analysed suggested an inflammatory response. No bacteria were isolated from the hematopoietic organs. However, Vibrio species, components of the normal seawater flora, were isolated from the tumour, which may have had a role in eliciting the immune response. No evidence of viral pathogens was found by electron microscopy. In order to look for cytogenetic alterations often linked to sarcomas, the diploid number and karyotype of this species were determined for the first time. An increase in the aneuploidy level was observed in sarcoma cell metaphase stages compared to other tissues. The aetiology of this tumour remains unknown.

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“…A positive correlation between season and immune parameters like in serum lysozyme level, respiratory burst activity, blood cell count and antibody titre was found in rainbow trout, Oncorhynchus mykiss, while the haematocrit value was negative [36]. Atlantic halibut also showed significantly different lysozyme levels between summer and winter [37].…”

Section: Discussionmentioning

confidence: 91%

Haematological and innate immune responses in Puntius sarana: normal range and seasonal variation

2012

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A study was conducted to measure the normal ranges, seasonal and annual variations in haematological and immune parameters of juvenile healthy Puntius sarana (weighing 75–100 g) during three major seasons over two consecutive years. Significantly (P<0.05) lower serum myeloperoxidase and ceruloplasmin activities, superoxide production, plasma glucose level, packed cell volume and haemoglobin concentration were detected in winter as compared to the summer season. However, serum lysozyme activity, antiprotease activity, total erythrocyte count (TEC) and TLC profiles were consistent during all seasons. The reference intervals (25th–75th) of each parameter were estimated and a range was established. The annual changes in immune parameters with minimum and maximum values were measured and a significant variation was noticed in myeloperoxidase, antiprotease, total protein levels and TEC over two consecutive years. The lower levels in haematological and innate immune status of fish during winter possibly indicate a higher disease risk period for the species.

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Seasonal variation of serum lysozyme levels in Atlantic halibut (Hippoglossus hippoglossus L.)

Cited by 49 publications

References 20 publications

Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>)

Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>)

Sarcoma in the thymus of juvenile meagre Argyrosomus regius reared in an intensive system

Haematological and innate immune responses in Puntius sarana: normal range and seasonal variation

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Seasonal variation of serum lysozyme levels in Atlantic halibut (Hippoglossus hippoglossus L.)

Cited by 49 publications

References 20 publications

Dietary selenium requirement of yellowtail kingfish (&lt;i&gt;Seriola lalandi&lt;/i&gt;)

Dietary selenium requirement of yellowtail kingfish (&lt;i&gt;Seriola lalandi&lt;/i&gt;)

Sarcoma in the thymus of juvenile meagre Argyrosomus regius reared in an intensive system

Haematological and innate immune responses in Puntius sarana: normal range and seasonal variation

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Product

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Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>)

Dietary selenium requirement of yellowtail kingfish (<i>Seriola lalandi</i>)