Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
17
0
3

Year Published

2012
2012
2020
2020

Publication Types

Select...
6
1
1

Relationship

2
6

Authors

Journals

citations
Cited by 25 publications
(20 citation statements)
references
References 46 publications
0
17
0
3
Order By: Relevance
“…Moreover, the number of genes analyzed in these studies was restricted by the use of Sanger-based cDNA sequencing and/or nucleic acid hybridization techniques. Such methodologies were also applied in large-scale gene expression analyses in M. perniciosa, providing initial insight into the pathogen's biology Pires et al, 2009;Leal et al, 2010). Nevertheless, these studies addressed the transcriptional regulation of M. perniciosa exclusively under artificial conditions, and the genetic program governing the fungal interaction with cacao remains unexplored.…”
Section: Introductionmentioning
confidence: 99%
“…Moreover, the number of genes analyzed in these studies was restricted by the use of Sanger-based cDNA sequencing and/or nucleic acid hybridization techniques. Such methodologies were also applied in large-scale gene expression analyses in M. perniciosa, providing initial insight into the pathogen's biology Pires et al, 2009;Leal et al, 2010). Nevertheless, these studies addressed the transcriptional regulation of M. perniciosa exclusively under artificial conditions, and the genetic program governing the fungal interaction with cacao remains unexplored.…”
Section: Introductionmentioning
confidence: 99%
“…External symptoms evolve to tissue necrosis, from where basidiocarps emerge. Control of this fungus is hard and expensive due to the cocoa crops conditions: high humidity, tropical forest environment, high cost fungicides, short interval applications due to perseverant raining, perennial crop, traditional methods of farming [11] Leal et al 2010. The objectives of this work were to test natural extracts from Solanum lycopersicon and purify such extracts to prove the growth inhibition of M. perniciosa and the specificity of such compound to this fungus.…”
Section: Introductionmentioning
confidence: 99%
“…Assim, os genes de patogenicidade são mais expressos sob carência de N, sendo esta tendência reforçada pela identificação na região promotora de genes de patogenicidade, de sequências específicas para o acoplamento de fatores de transcrição relacionados com metabolismo de N em fungos e bactérias (SNOEIJERS et al, 2000). Na busca por candidatos a genes de virulência e patogenicidade de M. perniciosa, foi realizada a procura em biblioteca de genes induzidos em cultivo sob a carência de N e sob fotoperíodo (LEAL Jr. et al, 2010). Esta estratégia vem sendo utilizada na identificação destes tipos de genes candidatos de virulência e patogenicidade, principalmente no cultivo in vitro sob condições de estresse abiótico, tais como restrição de carbono ou nitrogênio, presença de luz ou alteração de pH (PIETERSE et al, 1994;TALBOT et al, 1997;TRAIL et al, 2003;ACKERVEKEN et al, 1994;PRUSKY;YAKOY, 2003 (VERBENE et al, 2000).…”
Section: Microssatélites Em Fungosunclassified
“…Foi avaliada a expressão de sete genes presumíveis de patogenicidade e outras seis sequências selecionadas (LEAL Jr., 2006;LEAL Jr. et al, 2010) Os treze genes presumíveis associados à virulência foram então empregados para análise de expressão gênica por amplificação quantitativa de transcritos reversos (RT-qPCR).…”
Section: Amplificação Enzimática Quantitativa De Transcritos Reversosunclassified
See 1 more Smart Citation