Sea cucumber species identification of family Caudinidae from Surabaya based on morphological and mitochondrial DNA evidence

Amin, Mochamad; Pidada, I.B. Rai; Sugiharto, Sugiharto; Widyatmoko, Johan Nuari; Irawan, Bambang

doi:10.1063/1.4943311

Cited by 8 publications

(10 citation statements)

References 8 publications

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“…The identification of "teripang" in this study showed similarity to Acaudina sp. These results were similar to those of Amin and co-workers (2016) who identified the species using morphology and molecular identifications (Amin et al 2016). In addition, the identification of P. graeffei is consistent with local name reported by Setyastuti and Purwati (2015).…”

Section: Discussionsupporting

confidence: 91%

“…Other research groups have identified Indonesian fresh sea cucumbers using a molecular method by employing the cytochrome c oxidase subunit I (COI) region. Madduppa et al (2017) identified seven species of sea cucumbers from Kepulauan Seribu, meanwhile (Amin et al 2016) identified one species of sea cucumber from Surabaya. The advantage of using COI over the regular method is its resistance to the degradation from heat treatment (Rasmussen and Morrissey 2008).…”

Section: Introductionmentioning

confidence: 99%

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Identification of Beche-de-mers from Indonesia by molecular approach

et al. 2019

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Abstract. Patantis G, Dewi AS, Fawzya YN, Nursid M. 2019. Identification of Beche-de-mers from Indonesia by molecular approach. Biodiversitas 20: 537-543. Sea cucumbers have been commercially traded around the world due to their nutritional values and health benefits. Sea cucumbers are marketed as processed sea cucumber, also known as beche de mer, teripang, haisen or gamat. Due to the high global demand of beche-de-mers, there have been several cases of fraud due to the visual shape and color similarities of processed sea cucumbers. Additionally, the local name diversity of Indonesian sea cucumbers making the identification of beche-de-mers difficult. Therefore, this study aimed to identify commercial sea cucumbers from selected locations in Indonesia by molecular approach. The targeted Cytochrome c oxidase subunit I (COI) was used in this research. A total of 25 beche-de-mers was purchased from local markets in Indonesia, i.e. Boalemo (17 local names), Pesawaran (5), Surabaya (2) and West Lombok (1). Results showed that 22 species of beche-de-mer were successfully identified, ie. Acaudina sp., Actinopyga echinites, A. lecanora, A. miliaris, Bohadschia argus, B. marmorata, B. ocellata, B. vitiensis, Holothuria sp. H. atra, H. coluber, H. edulis, H. excellens, H. fuscocinerea, H. fuscogilva, H. fuscopunctata, H. scabra, Pearsonothuria graeffei, Phyrella sp., Stichopus herrmanni, S. horrens and S. monotuberculatus. The identification of Indonesian beche-de-mers using molecular method could support the identification of the local sea cucumbers to overcome mislabeled and fraud. Correct identification is important for the record of beche-de-mer trade in Indonesia and further conservation management.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Identification of Beche-de-mers from Indonesia by molecular approach

et al. 2019

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“…This Sea potato showed similarity with Acaudina sp. from Surabaya [20]. The sea cucumbers which have pink, smooth, and almost translucent body were also found in the soft bottom of the Wulan Estuary and were identified as A. molpadioides, Semper, 1868 (Figure 2F).…”

Section: Resultsmentioning

confidence: 97%

Sea cucumber species found in Soft-bottom of Wulan Estuary-Demak, Central Java

Hartati

Redjeki

Riniatsih

et al. 2021

IOP Conf. Ser.: Earth Environ. Sci.

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Indonesia has been known to have a high diversity of sea cucumber, but data and information are mostly on high-economic-value species. Sea cucumber living in soft-bottom substrates is less studied and unexploited. Wulan estuary located in the Demak Regency of Central Java was experiencing high sedimentation, which was consisted of fine material, and acted as a habitat for many macrozoobenthos, amongst others sea cucumbers. The objective of the present research was to identify the species of sea cucumber in the soft bottom of the Wulan estuary. The collection of sea cucumber specimens was done using a dredge. A total of 132 specimens were collected, examined, and identified through their morphological characters. The holothuroids found in the soft-bottom of the Wulan estuary represent five genera (Acaudina, Colochirus, Holothuria, Paracaudina, Phyllophorus,) in four families (Caudinidae, Cucumariidae, Holothuriidae, and Phyllophoridae) belonging to three orders (Molpadida, Dendrochirotida, and Aspidochirotida). The species were Acaudina sp., A. molpadioides, Colochirus quadrangularis, Holothuria cf. impatiens, Paracaudina sp., Paracaudina chilensis, P. australis, and Phyllophorus spiculata. A. molpadioides is presented as the most frequently found species. Although sea cucumber found in the Wulan estuary has not been exploited, it still needs to be managed to sustain its population.

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“…Total genomic DNA was extracted from about 30 mg of sea cucumbers muscle tissue using TIANamp Marine Animals DNA kit (TIANGEN, China) and purified using ZYMO Genomic DNA Clean & Concentrator kit (ZYMO, USA) according to the manufacture's protocols. Two mitochondrial fragments of 16S rRNA and COI gene were amplified using primers: 5´-CGCCTGTTTATCAAAAACAT-3´(16SAR), 5´-CTCCGGTTTGAACTCAGATCA-3´ (16SBR) (Kerr et al 2005) and 5´-ACTGCCCACGCCCTAGTAATGATATTTTTTATGGTNATGCC-3´ (COIce-F), 5´-TCGTGTGTCTACGTCCATTCCTACTGTRAACATRTG-3´ (COIceR) (Amin, et al 2016), respectively. The nuclear gene of H3 was amplified using primers: 5´-ATGGCTCGTACCAAGCAGACVGC-3´(H3a) and 5´-ATATCCTTRGGCATRATRGTGAC-3´ (H3b) (Colgan et al 1998).…”

Section: Methodsmentioning

confidence: 99%

Benthodytes marianensis, a new species of abyssal elasipodid sea cucumbers (Elasipodida: Psychropotidae) from the Mariana Trench area

Xiao

Zhang

et al. 2018

Zootaxa

759

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Benthodytes marianensis sp. nov., is described from the Mariana Trench at the depth of 5567 m. This is the thirteenth species in Benthodytes Théel, 1882. The new species has a broad brim around the body, dark violet skin, 9 pairs of large dorsal papillae with other papillae of equal or smaller size situated elsewhere, forming two zigzag rows, and some minute papillae scattered around the anterior region. Body wall ossicles are rods, crosses with three or four arms and central apophyses, and crosses of an unusual type. Crosses with four arms and central bipartite, tripartite and tetrapartite apophyses and peculiar cross-shaped ossicles with high apophyses ending in two horizontal arms are present in the dorsum. Large rod-shaped ossicles with bipartite apophyses and crosses with three or four arms and rudimentary apophyses are present on the ventrum. B. marianensis is morphologically most similar to B. incerta, but it is distinguished from B. incerta by the number and arrangement of dorsal large papillae, the broad brim and details of ossicle morphology. The phylogenetic analyses based on (16S, COI) and nuclear genes (H3) all confirm the morphological identification.

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Sea cucumber species identification of family Caudinidae from Surabaya based on morphological and mitochondrial DNA evidence

Cited by 8 publications

References 8 publications

Identification of Beche-de-mers from Indonesia by molecular approach

Identification of Beche-de-mers from Indonesia by molecular approach

Sea cucumber species found in Soft-bottom of Wulan Estuary-Demak, Central Java

Benthodytes marianensis, a new species of abyssal elasipodid sea cucumbers (Elasipodida: Psychropotidae) from the Mariana Trench area

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