Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2011.12.089 View full text
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M. Ashraf-Khorassani, L.T. Taylor, E. Seest

Abstract: A thorough evaluation of 5 μm bare silica from two major vendors for achiral supercritical fluid chromatography of polar analytes has been carried out. Columns were the same dimension, and a virgin column was reserved for each modifier-mixture combination. Three mixtures were prepared and chromatographically separated via a gradient of methanol-modified CO(2) that incorporated 5% (w/w) water as a neutral additive. Mixture (A) invoked both trifluoroacetic acid and water as additives. Mixture (B) utilized isopro…

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