Screening of strains and recombinant enzymes from Thermus thermophilus for their use in disaccharide synthesis

Characterization of the interaction between superoxide dismutase and 2-oxoisovalerate dehydrogenase

“…T. thermophilus is found in hot springs of neutral and alkaline pH, as well as in hot water or in natural waters subjected to thermal pollution and grows optimally at temperatures around 70-75°C (Fuciños et al, 2005). The thermophile produces a number of enzymes of considerable biotechnological interests, such as proteases, phosphatases, catalases, several DNA processing enzymes and lipases, which have been extensively documented (Pantazaki et al, 2002;Fuciños et al, 2005;Song and Zhang, 2008;Fuciños et al, 2011;Liu et al, 2011;Serrano-Posada et al, 2011;Sandoval et al, 2012;Song et al, 2012;Torres et al, 2012;Murugapiran et al, 2013). Although these enzymes play important roles in the thermoadaptation of thermophiles, the pathways mediated by them remain unclear.…”

Sustainable synthesis of N-acetyllactosamine using an immobilized β-galactosidase on a tailor made porous polymer

“…Spectra were consistent with previous references. 57 Re-use cycles. Aer the reaction was stopped, the immobilized biocatalyst was recovered, washed and re-assayed with fresh substrate mixture under the same conditions as in the rst experiment, and then the process was repeated twenty times.…”

“…Spectra were identical to the ones previously reported. 57 Re-use of the immobilized Biolacta-poly(AGE-co-DVB)-27 in the enzymatic synthesis of N-acetyl lactosamine with biosolvents…”

Revisiting the fundamentals of p-nitrophenol analysis for its application in the quantification of lipases activity. A graphical update.

“…Many analytical determinations using pNP release for enzyme-catalyzed reactions in aqueous solutions are based on reaction quenching by enzyme denaturation using alkaline reagents such as Na 2 CO 3 or NaOH (Lam, Cortez, Nguyen, Kato, & Cheruzel, 2016;Sandoval et al, 2012). This is a useful alternative when Figure 6.…”

“…wavelength, for the appropriate determination of this compound, since the absorbance of this chromophore remains without change regardless the pH. Another option for the accurate determination of the p-nitrophenol released by this reaction is the use of organic solvents (such as ethanol, acetone and other organic mixtures) as alternative quenchers, since they do not interfere in the analytical quantification of pNP and do not favor the hydrolysis of pNP esters (Lam et al, 2016;Palacios et al, 2014;Sandoval et al, 2012).…”