Screening of Nutritional Parameters for the Production of Protease from <i>Aspergillus Oryzae</i>

Gedela, Srinubabu; Lokeswari, N.; Jayaraju, K.

doi:10.1155/2007/915432

Cited by 25 publications

(19 citation statements)

References 7 publications

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“…Our report is in accordance with Srinubabu et al (2007) who also reported di-ammonium hydrogen orthophosphate for protease production by A. oryzae, whereas, Johnvesly and Naik (2001) observed nitrates as best nitrogen sources.…”

Section: Discussionsupporting

confidence: 92%

“…Glucose has been universally suggested as the best carbon source (Mehrotra et al, 1999, Srinubabu et al, 2007 as it can be easily metabolized by the microorganisms (Ashour et al, 1996). Adequate amount of enzyme (2054 U/gss) was produced by the isolated strain with glucose, however, sorbitol was found to be best source and maximum yield of enzyme (2531 U/gss) was obtained at a concentration of 200 mg/gss, while the inhibitory effect at higher concentrations might be due to catabolite repression.…”

Section: Discussionmentioning

confidence: 99%

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Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Santosh¹,

Bisht²,

Shikha³

et al. 2011

Afr. J. Biotechnol.

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The increase in agricultural practices has necessitated the judicious use of agricultural wastes into value added products. In this study, an extracellular, organic solvent and oxidant stable, serine protease was produced by Aspergillus flavus MTCC 9952 under solid state fermentation. Maximum protease yield was obtained when the strain was grown under wheat bran and corn cob mixture (1:1) incubated for 48 h at pH 9.0 and temperature 37°C with 50% of initial moisture content. The partially purified enzyme showed wide range of pH optima (8.0-12.0) and pH stability (7.0-12.0), whereas, optimum temperature was 40°C and was stable over a wide range of temperature 30-45°C. The protease was extremely stable towards several organic solvents. The enzyme retained 80% of its original activity in the presence of non ionic and ionic surfactants and 100% with 10% H 2 O 2 after 1 h of incubation at 30°C. In addition, the enzyme showed excellent compatibility with some commercial powder detergents. The compatibility of our protease with several detergents, oxidants and organic solvents suggests its possible use in detergent industry and peptide synthesis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Santosh¹,

Bisht²,

Shikha³

et al. 2011

Afr. J. Biotechnol.

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“…As the agitation rate was increased above 120 rpm, the enzyme production decreased. At this speed, aeration of the culture medium was increased which could lead to sufficient supply of dissolved oxygen in the media [17].…”

Section: Effect Of Agitation Rate On Protease Productionmentioning

confidence: 99%

Optimization of Process Parameters for Production of Alkaline Protease by OVAT Method Using Isolated Strain Alternaria alternata TUSGF1

Polley¹,

Ghosh²

2019

AFSJ

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Aim: The current study aimed at studying the optimum fermentation conditions and nutritional conditions for alkaline protease production by submerge fermentation using Alternaria alternata TUSGF1, isolated from poultry farm soil. Study Design: The results of environmental and nutritional parameters for protease production by OVAT method was analyzed by origin 6.1 software. Place and Duration of Study: Department of Food technology and Biochemical Engineering, Jadavpur University, Kolkata, West Bengal, India between March 2017 and May 2017. Methodology: A protease producing microorganism was isolated from a poultry farm soil and identified as Alternaria alternata TUSGF1. Various environmental and nutritional process parameters such as volume of medium, fermentation time, temperature, age of inoculums, agitation and carbon sources and nitrogen sources were standardized for the maximum yield of alkaline protease. Results: The optimum conditions of protease activity was 30°C at volume of medium 60 ml with 7 days age of inoculum in the medium containing 168 h of incubation and 120 rpm agitation rate. Peptone, casien, skimmed milk, urea and yeast extract were good nitrogen sources whilst maltose, fructose, starch, and sucrose were appropriate for enzyme production by submerge fermentation. Conclusion: Alkaline protease production by a newly isolated Alternaria alternata TUSGF1 from poultry farm soil was studied in shake flask conditions by submerge fermentation. It was established that the optimum protease production was recorded at 30°C, 60 ml volume of medium leaves and incubation time of 168 h. The best carbon and nitrogen sources for protease production were fructose and casein, respectively.

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“…Starch concentration decreased along with mold growth as sugar production increased.Aspergillus nigergrowth reached the end of the exponential phase at the 48 th hour. The maximum concentration of sugar achieved was 14.42 g/L.Aspergillus nigeralso consumes fats (Falony et al, 2006) and protein (Srinubabu et al, 2007).If polysaccharide content in the cultivation media is low, residual starch is reduced drastically.…”

Section: Cultivation Of Aspergillus Nigermentioning

confidence: 99%

Bioethanol Production from Tofu Waste by Simultaneous Saccharification and Fermentation (SSF) using Microbial Consortium

Febrianti¹

2017

IJTech

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Tofu waste can be used as a raw material for bioethanol production due to its high carbohydrate content in the form of starch. A microbial consortium, consisting of Aspergillus niger and Saccharomyces cerevisiae. The study's first objective wasto capture the amount of sugar produced from starch hydrolysis using single cultures of Aspergillus niger.The study's second objective wasto determine the amount of ethanol produced by the SSF technique. Aspergillus niger was used to produce an amylase enzyme that hydrolyzes starch into simple sugar.Then, Saccharomyces cerevisiae was used to produce bioethanol from the sugar produced earlier.The synthesis of bioethanol consists of two main stages, hydrolysis and fermentation. In previous studies, the hydrolysis and fermentation processes were performed separatelyusing a separated hydrolysis and fermentation (SHF)technique. This studyprocesses via a simultaneous saccharification and fermentation (SSF) technique which produced higher substrate efficiency, cell yield, and product yield compared to the SHF process.The characterization process showed that tofu waste flour was mainly composed of carbohydrates, which comprised 54.04±0.03% (dw) and had a starch content of 39.23±0.20 (dw). Sugar from the starch of the tofu waste was produced by batch system cultivation for 84 hours using Aspergillus niger. The highest sugar production (14.48 g/L) was achieved during the 48 th hour. Then, Saccharomyces cerevisiae was used to convert the produced sugar into bioethanol. The production of bioethanol by SSF using a microbial consortium for 72 hours was 7.69 g/L of bioethanol, with a yield of bioethanol per substrate use (Yp/s) of 0.23 g ethanol/g substrate and a substrate conversion efficiency of 88%.

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Screening of Nutritional Parameters for the Production of Protease from Aspergillus Oryzae

Cited by 25 publications

References 7 publications

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Oxidant and solvent stable alkaline protease from Aspergillus flavus and its characterization

Optimization of Process Parameters for Production of Alkaline Protease by OVAT Method Using Isolated Strain Alternaria alternata TUSGF1

Bioethanol Production from Tofu Waste by Simultaneous Saccharification and Fermentation (SSF) using Microbial Consortium

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