Screening of Five Specified Raw Materials in Several Imported Commercially Available Processed Foods Using Immunochromatography Kits

Suzuki, Tsuyoshi; Matsuura, Kenji; Maeda, Mamoru; Nagasaki, Toshio; Segami, Sathiko; Watanabe, Mithiko; Watanabe, Kenji; Shiroyanagi, Rieko; Nishida, Kanako; Toyoda, Masatake

doi:10.3136/nskkk.51.691

Cited by 3 publications

(1 citation statement)

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“…Analysis is initiated by dropping the protein solution extracted from a food onto a test strip, and a target band is detected after 10 to 20 minutes if antigen-antibody reaction turns positive, which is similar to ELISA and Western blotting methods. This method does not require expensive equipment/materials and can be visualized easily and quickly [22][23][24] . However, false-negatives are possible due to insufficient sensitivity, the influence of contaminants, and the hook effect or prozone effect (which occurs when excessive amounts of target protein are present).…”

Section: Immunochromatography Methods (Lateral Flow Method)mentioning

confidence: 99%

Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds

Akiyama

Adachi

2021

Food Safety

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In the Japanese allergy-labeling system, food labeling is mandated for 7 specific ingredients (egg, cow's milk, wheat, buckwheat, peanut, shrimp, and crab) and recommended for 21 food ingredients in reference to case numbers of actual illness and the degree of seriousness. To monitor the validity of the labeling system, official methods for the detection of specific ingredient proteins in processed foods were developed. The official methods consist of ELISA methods for screening, and western blot methods for egg and milk, and PCR methods for wheat, buckwheat, peanut, shrimp/prawn, and crab as confirmation tests. The official methods consist of ELISA methods for screening, and western blot methods for egg and milk, and PCR methods for wheat, buckwheat, peanut, shrimp/prawn, and crab as confirmation tests. Threshold amounts (a few mg/ kg) for labeling were set based on the approach of the analytical detections. Any foods containing protein allergens should be labeled if these contain allergens at greater than 10 ppm (mg/kg). Validation protocol criteria were established to standardize the Japanese official method. Food Safety Commission of Japan conducted a risk assessment of egg as a specific ingredient and judged that current labeling system for foods containing allergens is generally appropriate for "eggs". In the future, it is important to accumulate necessary scientific knowledge in order to carry out food health impact assessment including further refinement. The Japanese experience and knowledge of food allergy-labeling system would contribute to harmonize international labeling guidelines to protect allergic consumers globally.

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Section: Immunochromatography Methods (Lateral Flow Method)mentioning

confidence: 99%

Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds

Akiyama

Adachi

2021

Food Safety

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Evaluation of Immunochromatographic Test Kits for Food Allergens Using Processed Food Models

Morishita¹,

Akiyama²,

Arikawa³

et al. 2006

J. Food Hyg. Soc. Jpn

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Application of Two Immunochromatographic Test Kits to Screening of Four Kinds of Allergic Substances in Imported Foods

Hirota¹,

Sawada²,

Suzuki³

et al. 2007

J. Food Hyg. Soc. Jpn

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We tested for four kinds of allergic substances (egg, milk, wheat and peanuts) in 52 imported processed foods using immunochromatographic test kits (ITK). ELISA was also employed to confirm the e#ectiveness of the ITK. Among 92 data from 23 samples, allergic substances were detected in 9 cases with one kind of ITK, but not with the ELISA test. Among 116 data from 29 samples, 6 were negative with one kind of ITK and but positive with the other ITK. These results suggested that these 4 kinds of allergic substances in imported foods can be detected by using a double-check method with two kinds of ITK.

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Screening of Five Specified Raw Materials in Several Imported Commercially Available Processed Foods Using Immunochromatography Kits

Cited by 3 publications

References 0 publications

Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds

Japanese Food Allergy-Labeling System and Comparison with the International Experience; Detection and Thresholds

Evaluation of Immunochromatographic Test Kits for Food Allergens Using Processed Food Models

Application of Two Immunochromatographic Test Kits to Screening of Four Kinds of Allergic Substances in Imported Foods

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