Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites

Ördög, Vince; Stirk, Wendy A.; Lenobel, René; Bancı́řová, Martina; Strnad, Miroslav; Staden, J. Van; Szigeti, Jenő; Németh, László

doi:10.1023/b:japh.0000047789.34883.aa

Cited by 116 publications

(76 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extracellular IAA production in this microalga is mainly associated with stress conditions. Higher IAA production was archieved when illuminance was set at 11000 lx (the highest level), in absence of CO 2 and stirring of culture medium, demonstrating that the luminous flux is relevant to the production of this biomolecule.…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Production of indole-3-acetic acid in the culture medium of microalga Scenedesmus obliquus (UTEX 393)

Prieto¹,

Córdoba²,

MONTENEGRO³

et al. 2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

O acido indolacético (IAA) é uma auxina natural e um dos principais reguladores do crescimento de plantas superiores. A quantidade de ácido indolacético extracelular produzida pela cultura de microalga verde Scenedesmus obliquus foi estabelecida por cromatografia líquida de alta eficiência (HPLC). Avaliou-se o efeito de três variáveis de processo na produção de IAA extracelular nestas culturas usando planejamento fatorial 2 3 para uma metodologia de screening. As variáveis de planejamento experimental foram agitação, iluminância (intensidade do fluxo luminoso) e fonte de carvão (mistura CO 2 /air v/v (%)) em três diferentes níveis. A concentração de IAA e a taxa de crescimento específico foram usadas como variáveis de resposta. As análises de HPLC mostraram que em condições experimentais, a quantidade de IAA que foi liberada no meio pela alga S. obliquus depende somente da variável iluminância. Os efeitos da luz e as concentrações de carvão no meio de cultura foram significantes (p < 0.05) para uma taxa de crescimento específico.The indoleacetic acid (IAA) is a natural auxin and one of the main growth regulators in higher plants. The extracellular indoleacetic acid (IAA) amount produced by the green microalga Scenedesmus obliquus culture was detected by high performance liquid chromatography (HPLC). It was evaluated the effect of three process variables in the extracellular IAA production in these cultures using 2 3 factorial design for screening methodology. The experimental design variables were agitation, illuminance (intensity of luminous flux) and carbon source (CO 2 /air v/v (%) mixture) at three different levels. The IAA concentration and the specific growth rate were used as response variables. The HPLC analyses showed that under experimental conditions, the IAA amount that is released to the medium by S. obliquus depends only on the illuminance variable. The light effects and the carbon concentrations in culture medium were significant (p < 0.05) for specific growth rate.

show abstract

Section: Discussionmentioning

confidence: 93%

“…[1][2][3] Several studies describe the potential use of these microorganisms to produce bioactive compounds of high values. [4][5][6][7] Current applications for the isolated chemical compounds from various kinds of algae are enormous, and within those, ecological and functional chemistry is outstanding.…”

Section: Introductionmentioning

confidence: 99%

Production of indole-3-acetic acid in the culture medium of microalga Scenedesmus obliquus (UTEX 393)

Prieto¹,

Córdoba²,

MONTENEGRO³

et al. 2011

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…MBS have been reported to induce higher yields, a better efficiency of nutrient use and resistance to various abiotic and biotic stresses in a wide range of annual and perennial crops, including grapevine Turan and Köse 2004;Norrie et al 2001). The mechanisms by which MBS affect cell metabolism are mainly through the physiological action of major and minor nutrients, amino acids, vitamins, and also cytokinins, auxin, and absicisic acid (ABA)-like growth substances (Ördög et al 2004;Stirk et al 2003;Durand et al 2003;Crouch and van Staden 1993;Crouch et al 1992). Therefore, their effect is a result of many components that may work synergistically at different concentrations, although the mode of action of seaweed extracts still remains quite unknown (Vernieri et al 2005;Fornes et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of ammonium and potassium root influxes by the application of marine bioactive substances positively affects Vitis vinifera plant growth

et al. 2007

View full text Add to dashboard Cite

The enhancing effect of three marine bioactive substances (MBS) -EXT1116, NA9158 and 251104 -on the absorption of ammonium and potassium by the root system and the growth of potted grapevine (Vitis vinifera L.) plants is reported. Root ion influxes were determined in vivo by the non-invasive vibrating probe technique. Treatment with MBS generally enhanced nutrient absorption only in the root region between 0.8 and 1.7 mm from the root apex. Among the three substances tested, EXT1116 was the most effective in terms of enhancing the absorption of both ions, with significantly higher values than those of the other two substances and the control. NA9158 and 251104 were more effective in improving ammonium absorption than potassium absorption, while NA9158 was the most effective MBS in enhancing both biomorphometric parameters (shoot length, number of leaves, visual assessment of root system) and dry weight. Based on these results, we suggest that a combination of NA9158 and EXT1116 may be useful in enhancing plant growth by combining the capacity of NA9158 to increase root biomass and that of EXT1116 to enhance mineral absorption.

show abstract

“…After biomass was separated by centrifugation (4000 g for 10 min), the pellet was collected in a flask containing sterile Milli-Q water and kept at room temperature for 48 hrs to further stimulate the synthesis of secondary metabolites. Subsequently, the biomass was freeze dried at -20°C for 24 hrs 23 . 0.5 g freeze dried biomass was suspended in 10 ml of methanol and the cells were ruptured by ultrasonication (Sartorius, Germany; model: BBI 8535108) for 30 sec.…”

Section: Preparation Of Extractsmentioning

confidence: 99%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

A freshwater microalga, Nostoc sp. isolated from Tamdil Wetland of Mizoram was tested for its algicidal, antibacterial and antifungal activity. Extracts were prepared with methanol, ethanol:water and dichloromethane:isopropanol and tested for their antimicrobial activity against nine microorganisms comprising of three chlorophycean algae (Chlorella vulgaris, Scenedesmus quadricauda and Selenastrum capricornatum), one cyanobacterium (Anabaena variabilis), three bacterial strains (Bacillus subtilis, Bacillus pumilus and Escherichia coli) and two fungal strains (Fusarium udum and Fusarium culmorum). Among the three extracts tested the methanol extract was found most effective on algal test organisms. The highest (60%) inhibitory effect was observed against A. variabilis by methanol extract followed by 31% inhibition by dichloromethane:isopropanol. When tested on bacterial strains, the highest inhibition zone (23.67±1.58mm) was observed in B. subtilis by dichloromethane:isopropanol extract followed by ethanol:water extract. In case of fungal activity, the highest inhibition zone (11.00±0.58mm) was observed in F. culmorum by dichloromethane:isopropanol extract and the rest of the extracts showed almost similar effect. MICs of each extract on all the organisms varied. The extracts were further analysed by UV-VIS Spectrophotometer, which reveals the presence of active compounds. Among the three extracts, methanol indicated the highest percentage inhibition and also obtained highest absorption spectra. So, this extract was analysed by GC-MS and we identified the presence of main components in the extract as 1,2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester, Phytol, n-Hexadecanoic acid, 9,12-Octadecadienoic acid, etc. Further study for purification of the potent compound will explain their usefulness in pharmaceutical and biotechnological industry.

show abstract

Screening microalgae for some potentially useful agricultural and pharmaceutical secondary metabolites

Cited by 116 publications

References 11 publications

Production of indole-3-acetic acid in the culture medium of microalga Scenedesmus obliquus (UTEX 393)

Production of indole-3-acetic acid in the culture medium of microalga Scenedesmus obliquus (UTEX 393)

Enhancement of ammonium and potassium root influxes by the application of marine bioactive substances positively affects Vitis vinifera plant growth

Untitled

Contact Info

Product

Resources

About