Screening and characterization of endophytic fungi of Panax ginseng Meyer for biocontrol activity against ginseng pathogens

Park, Young-Hwan; Chung, Jun Young; Ahn, Deok Jong; Kwon, Tae Ryong; Lee, Seong-Kyu; Bae, Inhwan; Yun, Hae Keun; Bae, Hyeun‐Jong

doi:10.1016/j.biocontrol.2015.07.012

Cited by 27 publications

(16 citation statements)

References 70 publications

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“…Thus, it is possible that a particular fungi living as an endophyte in host plant in one ecosystem can turn pathogenic in another ecological niche. Phoma terrestris , which was isolated as endophyte from FCG, is known as a phytopathogen that causes pink root in onion 20 . P .…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have been conducted to survey the endophyte-plant interaction with particular emphasis on the production of secondary fungal metabolites and their potential use in biological control perspective 16 , 17 . Previous studies report phylogenetic diversity and biocontrol potential of fungal endophytes in field-cultivated ginseng (FCG) 18 – 20 , whereas fungal endophytes in MCG are still poorly characterized. Therefore, characterization of the fungal community associated with MCG is extremely important to reveal the identity of unexplored endophytes and identify novel compounds for biological control.…”

Section: Introductionmentioning

confidence: 99%

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Fungal endophytes inhabiting mountain-cultivated ginseng (Panax ginseng Meyer): Diversity and biocontrol activity against ginseng pathogens

Park

Kim

Mishra

et al. 2017

Sci Rep

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Fungal endophytes isolated from mountain-cultivated ginseng (MCG, Panax ginseng Meyer) were explored for their diversity and biocontrol activity against ginseng pathogens (Alternaria panax, Botrytis cinerea, Cylindrocarpon destructans, Pythium sp. and Rhizoctonia solani). A total of 1,300 isolates were isolated from three tissues (root, stem and leaf) from MCGs grown in 24 different geographic locations in Korea. In total, 129 different fungal isolates were authenticated by molecular identification based on internal transcribed spacer (ITS) sequences. The fungal endophytes belonged to Ascomycota (81.7%), Basidiomycota (7.08%), Zygomycota (10%) and Unknown (1.15%), with 59 genera. Analysis of diversity indices across sampling sites suggested species abundance as a function of geographical and environmental factors of the locations. Shannon diversity index and richness in the different tissues revealed that root tissues are colonized more than stem and leaf tissues, and also certain fungal endophytes are tissue specific. Assessment of the ethyl acetate extracts from 129 fungal isolates for their biocontrol activity against 5 ginseng pathogens revealed that Trichoderma polysporum produces the antimcriobial metabolite against all the pathogens. This result indicates the promise of its potential usage as a biocontrol agent.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fungal endophytes inhabiting mountain-cultivated ginseng (Panax ginseng Meyer): Diversity and biocontrol activity against ginseng pathogens

Park

Kim

Mishra

et al. 2017

Sci Rep

Self Cite

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“…and the late blight pathogen, Phytophthora infestans , but did not report an effect of the endophyte on late blight; and Beauveria bassiana was reported as a potential biocontrol agent based only on in vitro studies against the pathogens (Park et al ., ; Reisenzein & Tiefenbrunner, ). These and other studies (Lee et al ., ; Li et al ., ; Vieira et al ., ; Park et al ., ; Kandel et al ., ; Zheng et al ., ) describe in vitro interactions against pathogens, but no impact of the antagonists against a given disease.…”

Section: Discussionmentioning

confidence: 99%

Significant in vitro antagonism of the laurel wilt pathogen by endophytic fungi from the xylem of avocado does not predict their ability to control the disease

2018

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The ascomycete Raffaelea lauricola causes laurel wilt, a lethal vascular disease of avocado, Persea americana, and other members of the Lauraceae plant family. Few effective control measures for laurel wilt exist and new measures are needed. In this study, biological control of the disease with endophytic fungi from avocado was examined. Thirty-two endophytes (24 operational taxonomic units or OTUs) isolated from the xylem of healthy trees (the infection court of R. lauricola) were evaluated against R. lauricola with in vitro dual-culture assays. Nine OTUs that showed strong in vitro antagonism of the pathogen were tested in planta against laurel wilt. In three greenhouse experiments, grafted avocado plants of Simmonds or Russell cultivars, which are both susceptible to laurel wilt, were inoculated with endophytes and, after 10-16 days, inoculated with the same isolate of R. lauricola that was used in the in vitro assays. Within 14 days of inoculation with R. lauricola, laurel wilt developed in plants that were not treated with endophytes (positive controls) but also developed in endophyte-treated plants to the extent observed in the positive controls (P = 0.05). The pathogen colonized plants rapidly and systemically, but endophytes generally did not colonize xylem more than 2 cm above the point at which plants were inoculated. Although the tested endophytes strongly antagonized the pathogen in vitro, this did not translate to an ability to reduce development of laurel wilt. The management of laurel wilt and other plant diseases with fungal endophytes is discussed.

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“…showed antimicrobial activity against three bacteria. The fungi Colletotrichum pisi, Fusarium oxysporum, Fusarium solani, Phoma terrestris, and another two unknown fungi showed antimicrobial activity against some of the pathogens of ginseng (Park et al, 2015). These data indicate endophytic fungi help synthesise bioactive agents used by plants as protection against other pathogens (Ayob and Simarani, 2016).…”

Section: Introductionmentioning

confidence: 85%

Antimicrobial activity of crude extracts of endophytic fungi from Oryctanthus alveolatus (Kunth) Kuijt (Mistletoe)

Sanay¹,

Armando²,

Hathyla³

et al. 2018

Afr. J. Microbiol. Res.

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The use of antibiotics has unleashed high bacterial resistance. This outcome triggered the urgent need for effective new antibacterial agents to treat infectious diseases. A promising source for the production of antibiotics and several other bioactive substances are endophytic fungi. These microorganisms inhabit in and bring benefits to living plant tissues. Thus, the aim of this paper was to know the endophytic fungi associated with Oryctanthus alveolatus (mistletoe) and assess their potential to inhibit pathogenic bacteria. A total of 86 endophytic fungi were isolated from the stems and leaves of O. alveolatus. Of these fungi, 29 were selected for the assessment of antimicrobial activity. The antimicrobial activity of the obtained extracts was evaluated using the agar diffusion method towards two Gram-positive pathogenic bacteria (Staphylococcus aureus and Staphylococcus epidermidis) and two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The extracts were tested at concentrations of 200, 500, 700, 800, 900 and 1000 µg.mL -1 . The antimicrobial test showed that two (COA 009 and 014) of the 29 extracts inhibited bacterial growth of at least one of the strains each, from both Gram-positive and Gram-negative bacteria. The extracts with the inhibitory activity were derived from the fungi Curvularia sp. (COA 009) and Diaporthe sp. (COA 014).

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Screening and characterization of endophytic fungi of Panax ginseng Meyer for biocontrol activity against ginseng pathogens

Cited by 27 publications

References 70 publications

Fungal endophytes inhabiting mountain-cultivated ginseng (Panax ginseng Meyer): Diversity and biocontrol activity against ginseng pathogens

Fungal endophytes inhabiting mountain-cultivated ginseng (Panax ginseng Meyer): Diversity and biocontrol activity against ginseng pathogens

Significant in vitro antagonism of the laurel wilt pathogen by endophytic fungi from the xylem of avocado does not predict their ability to control the disease

Antimicrobial activity of crude extracts of endophytic fungi from Oryctanthus alveolatus (Kunth) Kuijt (Mistletoe)

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