Screen-Printed Polyaniline-Based Electrodes for the Real-Time Monitoring of Loop-Mediated Isothermal Amplification Reactions

Abstract

Nucleic acid amplification testing is a very powerful method to perform efficient and early diagnostics. However, the integration of a DNA amplification reaction with its associated detection in a low-cost, portable, and autonomous device remains challenging. Addressing this challenge, the use of screen-printed electrochemical sensor is reported. To achieve the detection of the DNA amplification reaction, a real-time monitoring of the hydronium ions concentration, a byproduct of this reaction, is performed. Such measurements are done by potentiometry using polyaniline (PAni)-based working electrodes and silver/silver chloride reference electrodes. The developed potentiometric sensor is shown to enable the real-time monitoring of a loop-mediated isothermal amplification (LAMP) reaction with an initial number of DNA strands as low as 10 copies. In addition, the performance of this PAni-based sensor is compared to fluorescence measurements, and it is shown that similar results are obtained for both methods.

Reduced graphene oxide-polyaniline film as enhanced sensing interface for the detection of loop-mediated-isothermal-amplification products by open circuit potential measurement

“…The voltage dri caused by pH change of rGO-PANi lm is of À64 mV per pH unit which is comparable to those obtained from other polyaniline-based sensors in previously reported works. 12,18,19 Depending on the fabrication process of the electrodes, the slope of the calibration curves ranges from À55 to À90 mV per pH unit. Compared to pristine polyaniline lm (Fig.…”

“…It should be emphasized that the measurement of the OCP is highly sensitive and reproducible. Indeed, although such a detection of LAMP products has been shown previously with potentiometric measurement, 12 however, this method could trigger signicant oxidation state change of PANi during current/potential imposition measurement, leading to inaccurate signals. By contrast, our OCP technique should provide a simpler and more accurate detection procedure since it can be done without any application of neither potential nor current to the system.…”

“…polyaniline) meet these criteria. 12,13 In the literature, it is well-known that polyaniline is a pH-sensitive lm with the response of about several tens millivolts per pH unit at room temperature. The pH sensitivity of polyaniline is due to the proton doping (protonation) and dedoping (deprotonation) of the conducting oxidative state, namely emeraldine.…”

Graphene Quantum Dots Decorated Gold-Polyaniline Nanowire for Impedimetric Detection of Carcinoembryonic Antigen

“…The continuous development of nanotechnology offers a new horizon for electrochemical detection of cancer makers. Several nanomaterials such as quantum dots 10 , metal nanoparticles 11 and conducting polymers 12 have been used to amplify the detection signal in electrochemical immunosensors. Among the nanostructured materials used, noble metal nanoparticles such as gold nanoparticle (AuNP) has attracted increasing attention and become a promising material for ultrasensitive detection of chemicals and biomolecules 13,14 have combined graphene and magnetic beads with enzyme-labelled antibody-AuNP bioconjugate for the electrochemical detection of CEA.…”

Combining 3D graphene-like screen-printed carbon electrode with methylene blue-loaded liposomal nanoprobes for phospholipase A2 detection

“…The electrochemical sensor based on screen-printed carbon electrode (SPCE) is particularly attractive because it is highly portable, disposable and cost-effective (Ping et al, 2012). To improve selectivity or increase sensitivity (Arduini et al, 2016), various inorganic and organic materials including copper (Zen et al, 2003), porous nickel (Niu et al, 2013), polyaniline (Gosselin et al, 2017), redox polymers (Gao et al, 2002), bacteriophage (Shabani et al, 2008), gold nano-particles (Labib et al, 2012), and reduced graphene (Scala-Benuzzi et al, 2018) have been used to modify the SPCEs. One approach is to create graphene-based electrochemical sensors that possess abundant binding-sites which could enhance signal detection because of its large accessible surface area and distinct electronic properties (Haque et al, 2012;Pumera, 2010;Zhou et al, 2009aZhou et al, , 2009b.…”