SOST, an LNGFR target, inhibits the osteogenic differentiation of rat ectomesenchymal stem cells

Abstract

SOST alleviated the osteogenic differentiation of EMSCs, and LNGFR enhanced the osteogenic differentiation of EMSCs by decreasing SOST, suggesting that the LNGFR/SOST pathway may be a novel target for promoting dental tissue regeneration and engineering.

The role and potential mechanism of p75NTR in mineralization via in vivo p75NTR knockout mice and in vitro ectomesenchymal stem cells

“…Moreover, micro‐CT and bone microstructural parameters analysis in p75NTR‐knockout mice showed obvious bone loss in both the femur trabecular and cortical bone, implying that the osteogenic potential was remarkably decreased in the absence of p75NTR. These in vivo data confirmed the findings of previous in vitro studies on the effect of p75NTR in mineralization . Mineralization‐related markers such as Runx2, ALP, Col1, BSP, OCN and OPN have been reported to display a positive correlation with p75NTR in rat EMSCs in vitro.…”

“…12 Moreover, p75NTR has been reported to participate in tooth morphogenesis and dental hard tissue mineralization. [13][14][15][16][17] However, most molecular signatures on the effect of p75NTR in tooth morphogenesis remain to be uncovered, and the elusive mechanism underlying tooth development has severely restricted dental tissue engineering thus far. In this study, p75N-TR ExIII -knockout mice and EMSCs isolated from p75NTR ExIII−/− and p75NTR +/+ male mice were investigated to reveal the exact mechanism of p75NTR in mineralization regulation, contributing to dental tissue engineering.…”

“…These in vivo data confirmed the findings of previous in vitro studies on the effect of p75NTR in mineralization. 13,17,18 F I G U R E 7 Investigations revealing the potential mechanism of p75NTR in mineralization. Immunohistochemistry showed similar expression patterns for p75NTR and Mage-D1 (A).…”

p75NTR−/− mice exhibit an alveolar bone loss phenotype and inhibited PI3K/Akt/β‐catenin pathway

“…Given the reduced bone mass and osteogenic differentiation in p75NTR −/− mice, we focused on embryonic stem cells to reveal the role of p75NTR in regulating craniomaxillofacial development.CNC-derived EMSCs, the progenitor cells of craniofacial hard tissues including the maxilla and mandible, populate the majority of the first branchial arch mesenchyme and play a crucial role in tooth and mandibular morphogenesis 20. In previous studies, we successfully developed rat EMSCs and detected their multi-differentiation abilities 10,37,38. In this study, EMSCs were isolated from WT and p75NTR −/− embryos separately, but derived from the same heterozygous pregnant mice.…”

Effect of Lactoferrin on the Expression Profiles of Long Non-coding RNA during Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells

“…Mechanism of MSCs osteogenic differentiation is regulated by complex pathways at transcriptional, post-transcriptional and post-translational levels [42,47,48,49,50], and the related osteogenesis signaling pathways, including TGF-β signaling [21], Wnt signaling [51], Hedgehog [52], Notch [53], NF-κappa B signaling [5], Ras [54], and Calcium Signaling [55]. In our study, a total number of 427 differential genes were identified and found to be related to osteogenic development pathways, such as MAPK signaling pathway, p53 signaling pathway (Cell growth and death), calcium signaling pathway and so on.…”