IV. Yeast sequencing reports. New open reading frames, one of which is similar to the nifV gene of Azotobacter vinelandii, Found on a 12·5 kbp fragment of chromosome IV of Saccharomyces cerevisiae

Abstract

The nucleotide sequence of a 12.5 kbp segment of the left arm of chromosome IV is described. Five open reading frames (ORFs) longer than 100 amino acids were detected, all of which are completely confined to the 12.5 kbp region. Two ORFs (D1271 and D1286) correspond to previously sequenced genes (PPH22 and VMA1 or TFP1, respectively). ORF D1298 shows the characteristics of alpha-isopropylmalate and homocitrate synthase genes and is similar to the nifV gene of Azotobacter vinelandii. Two more ORFs have no apparent homologue in the data libraries. Conversely, two smaller ORFs of 25 and 85 amino acids encoding the ribosomal protein YL41A and an ATPase inhibitor, respectively, were detected. Although a substantial part of the 12.5 kbp fragment apparently lacks protein-coding characteristics, no other elements, such as tRNA genes or transposons, were found.

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

“…typhimuriurn and E. coli enzymes) and eukaryotic origin (350/0 identity with Leu4p from S. cerevisiae). The highest similarity (84% identical amino acids) was observed with ORF D1298 of S. cerevisiae recently described as part of the yeast sequencing programme (Verhasselt et al, 1995), which probably encodes one of the two homocitrate synthase isoenzymes thought to be present in this species (Bhattacharjee, 1985;Tucci and Ceci, 1972). Conserved regions between all six polypeptides are distributed over the entire length, except in the N-terminal region where bacterial genes lack an N-terminal extension (Figure 3).…”

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

“…typhimuriurn and E. coli enzymes) and eukaryotic origin (350/0 identity with Leu4p from S. cerevisiae). The highest similarity (84% identical amino acids) was observed with ORF D1298 of S. cerevisiae recently described as part of the yeast sequencing programme (Verhasselt et al, 1995), which probably encodes one of the two homocitrate synthase isoenzymes thought to be present in this species (Bhattacharjee, 1985;Tucci and Ceci, 1972). Conserved regions between all six polypeptides are distributed over the entire length, except in the N-terminal region where bacterial genes lack an N-terminal extension (Figure 3).…”

In Saccharomyces cerevisae , feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p

“…The recent sequencing of chromosome IV of S. cerevisiae has allowed the identification of the gene LYS20 (YDL182w previously named D1278) encoding one isoform of homocitrate synthase [26]. We have shown that disruption of this gene does not prevent growth on minimal medium but leads to a reduced although significant level of homocitrate synthase, indicating that this activity is due to at least two isoenzymes ( [14]; this paper).…”

Lysine is synthesized through the α-aminoadipate pathway inThermus thermophilus

“…3). The predicted T. thermophilus LYS4 protein showed 30.4% identity with homoaconitate hydratase of S. cerevisiae [22].…”

“…The LYS20 gene (from nt 1031 to nt 2161) encodes 376 amino acids, and the LYS4 gene (from nt 2601, truncated) encodes more than 386 amino acids. The predicted T. thermophilus LYS20 protein showed significant homology to homocitrate synthase of S. cerevisiae [22] and Yarrowia lipolytica [23], with 53.5% and 52.7% identity (Fig. 3).…”

“… Amino acid sequence alignment of homocitrate synthases from T. thermophilus, S. cerevisiae [22], Y. lipolytica [23] and K. pneumoniae [25]. Amino acids conserved in three or more organisms are shaded.…”

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

“…typhimuriurn and E. coli enzymes) and eukaryotic origin (350/0 identity with Leu4p from S. cerevisiae). The highest similarity (84% identical amino acids) was observed with ORF D1298 of S. cerevisiae recently described as part of the yeast sequencing programme (Verhasselt et al, 1995), which probably encodes one of the two homocitrate synthase isoenzymes thought to be present in this species (Bhattacharjee, 1985;Tucci and Ceci, 1972). Conserved regions between all six polypeptides are distributed over the entire length, except in the N-terminal region where bacterial genes lack an N-terminal extension (Figure 3).…”

In Saccharomyces cerevisae , feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p

“…The recent sequencing of chromosome IV of S. cerevisiae has allowed the identification of the gene LYS20 (YDL182w previously named D1278) encoding one isoform of homocitrate synthase [26]. We have shown that disruption of this gene does not prevent growth on minimal medium but leads to a reduced although significant level of homocitrate synthase, indicating that this activity is due to at least two isoenzymes ( [14]; this paper).…”

Lysine is synthesized through the α-aminoadipate pathway inThermus thermophilus

“…3). The predicted T. thermophilus LYS4 protein showed 30.4% identity with homoaconitate hydratase of S. cerevisiae [22].…”

“…The LYS20 gene (from nt 1031 to nt 2161) encodes 376 amino acids, and the LYS4 gene (from nt 2601, truncated) encodes more than 386 amino acids. The predicted T. thermophilus LYS20 protein showed significant homology to homocitrate synthase of S. cerevisiae [22] and Yarrowia lipolytica [23], with 53.5% and 52.7% identity (Fig. 3).…”

“… Amino acid sequence alignment of homocitrate synthases from T. thermophilus, S. cerevisiae [22], Y. lipolytica [23] and K. pneumoniae [25]. Amino acids conserved in three or more organisms are shaded.…”

Cloning and sequencing of the LYS1 gene encoding homocitrate synthase in the yeast Yarrowia lipolytica

“…typhimuriurn and E. coli enzymes) and eukaryotic origin (350/0 identity with Leu4p from S. cerevisiae). The highest similarity (84% identical amino acids) was observed with ORF D1298 of S. cerevisiae recently described as part of the yeast sequencing programme (Verhasselt et al, 1995), which probably encodes one of the two homocitrate synthase isoenzymes thought to be present in this species (Bhattacharjee, 1985;Tucci and Ceci, 1972). Conserved regions between all six polypeptides are distributed over the entire length, except in the N-terminal region where bacterial genes lack an N-terminal extension (Figure 3).…”

In Saccharomyces cerevisae , feedback inhibition of homocitrate synthase isoenzymes by lysine modulates the activation of LYS gene expression by Lys14p

“…The recent sequencing of chromosome IV of S. cerevisiae has allowed the identification of the gene LYS20 (YDL182w previously named D1278) encoding one isoform of homocitrate synthase [26]. We have shown that disruption of this gene does not prevent growth on minimal medium but leads to a reduced although significant level of homocitrate synthase, indicating that this activity is due to at least two isoenzymes ( [14]; this paper).…”

Lysine is synthesized through the α-aminoadipate pathway inThermus thermophilus

“…3). The predicted T. thermophilus LYS4 protein showed 30.4% identity with homoaconitate hydratase of S. cerevisiae [22].…”

“…The LYS20 gene (from nt 1031 to nt 2161) encodes 376 amino acids, and the LYS4 gene (from nt 2601, truncated) encodes more than 386 amino acids. The predicted T. thermophilus LYS20 protein showed significant homology to homocitrate synthase of S. cerevisiae [22] and Yarrowia lipolytica [23], with 53.5% and 52.7% identity (Fig. 3).…”

“… Amino acid sequence alignment of homocitrate synthases from T. thermophilus, S. cerevisiae [22], Y. lipolytica [23] and K. pneumoniae [25]. Amino acids conserved in three or more organisms are shaded.…”