2018
DOI: 10.1111/tbed.13053
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Abstract: Brucellosis is a worldwide zoonosis causing important economic loss and a public health problem. Small ruminants are the preferred hosts of Brucella melitensis and thus the main source of human infections. Effective control of sheep and goat brucellosis has been achieved in several countries through vaccination with the live‐attenuated B. melitensis Rev1 vaccine. However, Rev1 induces a long‐lasting serological response that hinders the differentiation between infe… Show more

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Cited by 7 publications
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References 36 publications
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“…More recently, a Rev1 construct expressing the green fluorescent protein as a tagging antigen has been proposed. However, this method requires the simultaneous injection of this protein and a booster injection to trigger antibody persisting in the ancillary ELISA‐green fluorescent protein DIVA test [22].…”
Section: Introductionmentioning
confidence: 99%
“…More recently, a Rev1 construct expressing the green fluorescent protein as a tagging antigen has been proposed. However, this method requires the simultaneous injection of this protein and a booster injection to trigger antibody persisting in the ancillary ELISA‐green fluorescent protein DIVA test [22].…”
Section: Introductionmentioning
confidence: 99%
“…The Tn7 carrying the gfpmut3 gene was inserted in chromosome II of B . abortus S19 by using the four-parental mating method previously described [ 37 , 40 ].The expression of the gfpmut3 gene is driven by the E . coli rrnB P1 ribosomal promoter, as described in [ 39 ].…”
Section: Methodsmentioning
confidence: 99%
“…Endotoxicity of the purified GST-GFP was determined by Limulus lysate assay (Sigma-Aldrich), following product information technical bulletin. The stability and integrity of GST-GFP were tested as described previously [ 37 ].…”
Section: Methodsmentioning
confidence: 99%
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“…This is because the bacterium remains viable in the host for a certain time and replicates. This promotes an immune response mediated by T lymphocytes against components of the pathogen, guaranteeing its effectiveness as protection-inducing agent against brucellosis (84, 148).…”
Section: Live Attenuated Vaccinesmentioning
confidence: 99%