Schirmer strip vs. capillary tube method: Non-invasive methods of obtaining proteins from tear fluid

Posa, Andreas; Bräuer, Lars; Schicht, Martin; Garreis, Fabian; Beileke, Stephanie; Paulsen, Friedrich

doi:10.1016/j.aanat.2012.10.001

Cited by 177 publications

(189 citation statements)

References 45 publications

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“…The centrifugal force pulled the tear fluid out of the Schirmer strip, through the central ''pore'' in the bottom of the smaller tube and into the outer 1.5 mL tube. 20 …”

Section: Isolation Of the Tear Fluid From Schirmer Stripsmentioning

confidence: 99%

Palate Lung Nasal Clone (PLUNC), a Novel Protein of the Tear Film: Three-Dimensional Structure, Immune Activation, and Involvement in Dry Eye Disease (DED)

Schicht

Rausch

Beron

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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Citation: Schicht M, Rausch F, Beron M, et al. Palate lung nasal clone (PLUNC), a novel protein of the tear film: three-dimensional structure, immune activation, and involvement in dry eye disease (DED). Invest Ophthalmol Vis Sci. 2015;56:7312-7323. DOI:10.1167/iovs.15-17560 PURPOSE. Palate Lung Nasal Clone (PLUNC) is a hydrophobic protein belonging to the family of surfactant proteins that is involved in fluid balance regulation of the lung. Moreover, it is known to directly act against gram-negative bacteria. The purpose of this study was to investigate the possible expression and antimicrobial role of PLUNC at the healthy ocular surface and in tears of patients suffering from dry eye disease (DED).METHODS. Bioinformatics and biochemical and immunologic methods were combined to elucidate the structure and function of PLUNC at the ocular surface. Tissue-specific localization was performed by using immunohistochemistry. The PLUNC levels in tear samples from non-Sjögren's DED patients with moderate dry eye suffering either from hyperevaporation or tear deficiency were analyzed by ELISA and compared with tears from healthy volunteers.RESULTS. Palate Lung Nasal Clone is expressed under healthy conditions at the ocular surface and secreted into the tear film. Protein modeling studies and molecular dynamics simulations performed indicated surface activity of PLUNC. In vitro experiments revealed that proinflammatory cytokines and bacterial supernatants have only a slight effect on the expression of PLUNC in HCE and HCjE cell lines. In tears from DED patients, the PLUNC concentration is significantly increased (7-fold in evaporative dry eye tears and 17-fold in tears from patients with tear deficiency) compared with healthy subjects. CONCLUSIONS.The results show that PLUNC is a protein of the tear film and suggest that it plays a role in fluid balance and surface tension regulation at the ocular surface.

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“…The centrifugal force pulled the tear fluid out of the Schirmer strip, through the central ''pore'' in the bottom of the smaller tube and into the outer 1.5 mL tube. 20 …”

Section: Isolation Of the Tear Fluid From Schirmer Stripsmentioning

confidence: 99%

Palate Lung Nasal Clone (PLUNC), a Novel Protein of the Tear Film: Three-Dimensional Structure, Immune Activation, and Involvement in Dry Eye Disease (DED)

Schicht

Rausch

Beron

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…The procedure was performed as previously described by Posa et al considering the following modification (Posa et al, 2012). The protein content of these collected saliva samples, was measured with a protein assay based on the Bradford dye-binding procedure (BioRad, Hercules, CA).…”

Section: Western Blot Analysismentioning

confidence: 99%

The distribution of human surfactant proteins within the oral cavity and their role during infectious diseases of the gingiva

Schicht

Stengl

Sel

et al. 2015

Annals of Anatomy - Anatomischer Anzeiger

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“…Studies on drugs for ocular applications present many bioanalytical challenges, from the accurate collection of the entire eye, the eye compartment or secreted liquid (which requires highly trained scientists), to the correct storage conditions and preparation of the samples [44]. These factors all determine the quality of the final bioanalytical result.…”

Section: Challenges With Methods Development and Validation Of Drugs In mentioning

confidence: 99%

2017 White Paper on Recent Issues in Bioanalysis: Aren’t Bmv Guidance/Guidelines ‘ Scientific ’? (Part 1 – LCMS: Small Molecules, Peptides and Small Molecule Biomarkers)

Welink¹,

Yang

Hughes³

et al. 2017

Bioanalysis

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The 2017 11th Workshop on Recent Issues in Bioanalysis (11th WRIB) took place in Los Angeles/Universal City, California from 3 April 2017 to 7 April 2017 with participation of close to 750 professionals from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day, weeklong event – A Full Immersion Week of Bioanalysis, Biomarkers and Immunogenicity. As usual, it was specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small and large molecule analysis involving LCMS, hybrid LBA/LCMS and ligand-binding assay (LBA) approaches. This 2017 White Paper encompasses recommendations emerging from the extensive discussions held during the workshop, and is aimed to provide the bioanalytical community with key information and practical solutions on topics and issues addressed, in an effort to enable advances in scientific excellence, improved quality and better regulatory compliance. Due to its length, the 2017 edition of this comprehensive White Paper has been divided into three parts for editorial reasons. This publication (Part 1) covers the recommendations for Small Molecules, Peptides and Small Molecule Biomarkers using LCMS. Part 2 (Biotherapeutics, Biomarkers and Immunogenicity Assays using Hybrid LBA/LCMS and Regulatory Agencies’ Inputs) and Part 3 (LBA: Immunogenicity, Biomarkers and PK Assays) are published in volume 9 of Bioanalysis, issues 23 and 24 (2017), respectively.

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Schirmer strip vs. capillary tube method: Non-invasive methods of obtaining proteins from tear fluid

Cited by 177 publications

References 45 publications

Palate Lung Nasal Clone (PLUNC), a Novel Protein of the Tear Film: Three-Dimensional Structure, Immune Activation, and Involvement in Dry Eye Disease (DED)

Palate Lung Nasal Clone (PLUNC), a Novel Protein of the Tear Film: Three-Dimensional Structure, Immune Activation, and Involvement in Dry Eye Disease (DED)

The distribution of human surfactant proteins within the oral cavity and their role during infectious diseases of the gingiva

2017 White Paper on Recent Issues in Bioanalysis: Aren’t Bmv Guidance/Guidelines ‘ Scientific ’? (Part 1 – LCMS: Small Molecules, Peptides and Small Molecule Biomarkers)

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