Scents from Brazilian Cerrado: chemical composition of the essential oil from<i>Pseudobrickellia brasiliensis</i>(Asteraceae)

Silva, Rafael F.; Rezende, Cláudia M.; Pereira, Jonny Everson Scherwinski; Vieira, R. F.; Santos, M. C. S.; Bizzo, H. R.

doi:10.1080/10412905.2015.1037021

Cited by 5 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies with P. brasiliensis apolar extracts revealed the presence of triterpenes and sesquiterpenes, such as hidroxigermacreno, lupeol, β-amyrin acetate, spathulenol, cadinene, cadinol, oplopanona acetate, and α-acetoxy-β-amyrin ( 20 ). Moreover, the essential oil from the leaves contains the major compounds terpinen-4-ol, γ-terpinene, α-terpinene, and α-terpineol ( 21 ). However, no study has been conducted to identify the chemical compounds present in the polar extract of P. brasiliensis , a similar formulation used in folk medicine.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro

Almeida

Avelar-Freitas

Santos

et al. 2017

Braz J Med Biol Res

View full text Add to dashboard Cite

Pseudobrickellia brasiliensis (Asteraceae) is a plant commonly known as arnica-do-campo and belongs to the native flora of the Brazilian Cerrado. The alcoholic extract of the plant has been used as an anti-inflammatory agent in folk medicine, but the biological mechanism of action has not been elucidated. The present study evaluated the composition of P. brasiliensis aqueous extract and its effects on pro-inflammatory cytokine production and lymphocyte proliferation. The extracts were prepared by sequential maceration of P. brasiliensis leaves in ethanol, ethyl acetate, and water. Extract cytotoxicity was evaluated by trypan blue exclusion assay, and apoptosis and necrosis were measured by staining with annexin V-FITC and propidium iodide. The ethanolic (ETA) and acetate (ACE) extracts showed cytotoxic effects. The aqueous extract (AQU) was not cytotoxic. Peripheral blood mononuclear cells stimulated with phorbol myristate acetate and ionomycin and treated with AQU (100 μg/mL) showed reduced interferon (IFN)-γ and tumor necrosis factor (TNF)-α expression. AQU also inhibited lymphocyte proliferative response after nonspecific stimulation with phytohemagglutinin. The aqueous extract was analyzed by liquid chromatography coupled with photodiode array detection and mass spectrometry. Quinic acid and its derivatives 5-caffeoylquinic acid and 3,5-dicaffeoylquinic acid, as well as the flavonoids luteolin and luteolin dihexoside, were detected. All these compounds are known to exhibit anti-inflammatory activity. Taken together, these findings demonstrate that P. brasiliensis aqueous extract can inhibit the pro-inflammatory cytokine production and proliferative response of lymphocytes. These effects may be related to the presence of chemical substances with anti-inflammatory actions previously reported in scientific literature.

show abstract

Section: Resultsmentioning

confidence: 99%

Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro

Almeida

Avelar-Freitas

Santos

et al. 2017

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…This is the first time that the presence of kaurenoic acid in P. brasiliensis has been reported. There are studies that indicate some activities for kaurenoic acid and derivatives: anticonvulsant, anticancer, anti-inflammatory, and others (Okoye et al, 2013;Lima Silva et al, 2015;Simão et al, 2016). Chemotaxonomic studies have indicated the presence of terpenes in the Asteraceae family, and diterpenes with a kaurane skeleton was found in the Eupatorieae tribe (Alvarenga et al, 2005).…”

Section: Chemical Constituents In the Hexane And Ethyl Acetate Extractsmentioning

confidence: 99%

“…The whole plant or parts of the plant are popularly used as an analgesic and an antiinflammatory and topical wound healer in a tanned or macerated form in alcohol (Ribeiro et al, 2001;Silva et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Chemical constituents of Pseudobrickellia brasiliensis leaves(Spreng.) R.M. King & H. Rob. (Asteraceae)

Amorim

Godinho

Archanjo

et al. 2016

Rev. bras. plantas med.

View full text Add to dashboard Cite

Pseudobrickelliabrasiliensisis aspecies endemic toBrazil, popularlyknown as "arnica"/ "arnica-do-campo"/ "arnica-do-mato" and used for itsanalgesicand antiinflammatoryproperties. The objective of this research was thephytochemical studyof the essential oilandhexaneandethyl acetateextracts of the leaves of this species. The essential oilwasextracted byhydrodistillation using a Clevengerapparatusand was analyzed byGC/MS, 25components were identified, with a predominance ofmonoterpenes. The extractswere subjected toclassicalchromatographyand the fractionswere analyzed byGC/ MS, 1D 1 H-NMR, 13 C-NMR and 13 C-NMR-DEPT 135.α-amyrin, α-amyrin acetate, β-amyrin, β-amyrin acetate, lupeol, lupeolacetate, pseudotaraxasterol andtaraxasterol (triterpenes), andkaurenoicacid (diterpene) were identified.Theseterpenesarechemo-taxonomicallyrelated to theEupatorieaetribe(Asteraceae) and may be responsible for the anti-inflammatory activity attributed to the plant.Key words: Pseudobrickellia brasiliensis, Asteraceae, medicinal plant, phytochemistry, terpenes. RESUMO: Constituintes químicos das folhas de Pseudobrickellia brasiliensis (Spreng.) R.M. King & H. Rob. (Asteraceae).Pseudobrickellia brasiliensis é uma espécie endêmica do Brasil, popularmente conhecida como "arnica"/ "arnica-do-campo"/ "arnica-do-mato" e usada por suas propriedades analgésica e antiinflamatória. O objetivo do trabalho foi o estudo fitoquímico do óleo essencial e dos extratos hexânico e em acetato de etila das folhas dessa espécie. O óleo essencial foi extraído por hidrodestilação em aparato de Clevenger e foi analisado por CG/ EM, sendo identificados 25 componentes, com predomínio de monoterpenos. Os extratos foram submetidos a cromatografia clássica, e as frações foram analisadas por CG/EM, 1D 1 H-RMN, 13 C-RMN e 13 C-RMN-DEPT 135. Foram identificados α-amirina, acetato de α-amirina, β-amirina, acetato de β-amirina, lupeol, acetato de lupeol, pseudotaraxasterol e taraxasterol (triterpenos) e o ácido caurenóico (diterpeno). Estes terpenos estão quimiotaxonomicamente relacionados a tribo Eupatorieae (Asteraceae) e podem ser responsáveis pela atividade antiinflamatória atribuída a planta. Palavras-chave:Pseudobrickellia brasiliensis, Asteraceae, planta medicinal, fitoquímica, terpenos.

show abstract

Non-clinical investigations about cytotoxic and anti-platelet activities of gamma-terpinene

Souza,

Pimentel,

de Sousa

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

View full text Add to dashboard Cite

Gamma-terpinene (γ-TPN) is a cyclohexane monoterpene, isolated from essential oils of pharmacologically active plant species, such as tea tree (Melaleuca alternifolia), oregano (Origanum vulgare), rosemary (Rosmarinus o cinalis L.), thyme (Thymus vulgaris Marchand) and eucalyptus (Eucalyptus sp.). Terpenes are widely studied for their recognized pharmacological actions on the cardiovascular system, hemostasis and antioxidant actions. The objective of this study was to investigate the cytotoxic and antiplatelet activity of γ-TPN in non-clinical study models. For the in silico evaluation, the PreADMET, SwissADME and SwissTargetPrediction software were used. Molecular docking was performed using the AutoDockVina and BIOVIA Discovery Studio databases. The cytotoxicity of γ-TPN was analyzed by the MTT assay with normal murine endothelial (SVEC4-10) and broblast (L929) lines. Platelet aggregation was evaluated with platelet-rich (PRP) and platelet-poor (PPP) plasma from spontaneously hypertensive rats (SHR), in addition to SVEC4-10 cells pre-incubated with γ-TPN (50, 100 and 200 µM) for 24 h. In in vivo tests, SHR animals were also used, pre-treated by gavage with γ-TPN for 7 days, distributed into four groups (control, 25, 50 and 100 mg/Kg). At the end, blood samples were collected to measure nitrites using the Griess reagent. γ-TPN proved to be quite lipid-soluble (Log P = + 4.50), with a quali ed pro le of similarity to the drug, good bioavailability, and adequate pharmacokinetics. The monoterpene exhibited a nity mainly for the P2Y12 receptor (6.450 ± 0.232 Kcal/mol), moderate cytotoxicity for L929 (CC 50 = 333.3 µM) and SVEC 4-10 (CC 50 = 366.7 µM). The presence of γ-TPN in SVEC 4-10 cells was also able to reduce platelet aggregation by 51.57 and 44.20%, respectively, at the lowest concentrations (50 and 100 µM). It was concluded that γ-TPN has a good a nity with purinergic receptors and an effect on the reversal of platelet aggregation and oxidative stress, being promising and safe for therapeutic targets and subsequent studies in the control of thromboembolic diseases.

show abstract

Scents from Brazilian Cerrado: chemical composition of the essential oil fromPseudobrickellia brasiliensis(Asteraceae)

Cited by 5 publications

References 6 publications

Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro

Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M. King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro

Chemical constituents of Pseudobrickellia brasiliensis leaves(Spreng.) R.M. King & H. Rob. (Asteraceae)

Non-clinical investigations about cytotoxic and anti-platelet activities of gamma-terpinene

Contact Info

Product

Resources

About