In this study, the structural architecture of oil palm frond (OPF) after various pretreatment is evaluated through microscopic studies. Maximal degrees of saccharification of OPF are depended on pretreatment method used. Each pretreatment has its own effect(s) on the fiber of OPF. OPF is a solid waste generated by the palm oil industry that creates a problem for the industry in terms of waste management. This lignocellulosic waste material has many physio-chemical structural and compositional factors that hinder the enzymatic digestion of the cellulose present in the lignocellulosic biomass. A pretreatment is needed to alter or remove these structural and compositional impediments to hydrolysis in order to improve the rate of enzyme hydrolysis and increase the yield of fermentable sugars. The structural architecture of lignocellulosic fibres after various pretreatment e.g. chemical, biological and biochemical, were evaluated through light microscopic (LM), scanning electron microscopic (SEM) and transmission electron microscopic (TEM) studies. These pretreatments, both separately and intergrated, rendered the OPF biomass more susceptible and accessible to saccharification and increased the production of fermentable sugars. Morphological changes that took place in the lignocellulosic biomass included the removal of inhibitory materials, e.g. triterpenoids, silica, hydrocarbons, etc., production of cracks in the lignocellulosic fibres, and exposure of cellulosis materials by creating pores during pretreatment. The most effective pretreatment was autoclaving followed by enzymatic hydrolysis of the OPF. This combination of treatments resulted in a significant increase in reducing sugar production by creating pores due to the removal of silica and lignin from biomass, as observed in SEM studies.