Scale-up of mixing processes of highly concentrated suspensions using electrical resistance tomography

Lomtscher, Annett; Jobst, Karin; Fogel, Stefan; Rostalski, Kay; Stempin, Silke; Kraume, Matthias

doi:10.1016/j.flowmeasinst.2016.10.002

Cited by 20 publications

(14 citation statements)

References 6 publications

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“…However, in addition to the required improvements in phage purification, the standardization and phage production process has not been widely explored. Throughout the literature, there are references to the importance as well as the need for scale-up, yet studies exploring large-scale manufacture of phages are scarce (Krysiak-Baltyn, Martin, & Gras, 2018;Lomtscher et al, 2017;Warner, Barker, Lee, & Perkins, 2014). For successful translation of phage therapies into the clinic, scalable, robust, and cost-effective manufacturing processes are required to match the expected increased demand.…”

Section: Studies Have Shown That Approximately 54% Of Predominantmentioning

confidence: 99%

A scaled‐down model for the translation of bacteriophage culture to manufacturing scale

Ali

Rafiq

Ratcliffe

2019

Biotech & Bioengineering

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Therapeutic bacteriophages are emerging as a potential alternative to antibiotics and synergistic treatment of antimicrobial‐resistant infections. This is reflected by their use in an increasing number of recent clinical trials. Many more therapeutic bacteriophage is being investigated in preclinical research and due to the bespoke nature of these products with respect to their limited infection spectrum, translation to the clinic requires combined understanding of the biology underpinning the bioprocess and how this can be optimized and streamlined for efficient methods of scalable manufacture. Bacteriophage research is currently limited to laboratory scale studies ranging from 1–20 ml, emerging therapies include bacteriophage cocktails to increase the spectrum of infectivity and require multiple large‐scale bioreactors (up to 50 L) containing different bacteriophage–bacterial host reactions. Scaling bioprocesses from the milliliter scale to multi‐liter large‐scale bioreactors is challenging in itself, but performing this for individual phage‐host bioprocesses to facilitate reliable and robust manufacture of phage cocktails increases the complexity. This study used a full factorial design of experiments approach to explore key process input variables (temperature, time of infection, multiplicity of infection, agitation) for their influence on key process outputs (bacteriophage yield, infection kinetics) for two bacteriophage–bacterial host bioprocesses (T4 – Escherichia coli; Phage K – Staphylococcus aureus). The research aimed to determine common input variables that positively influence output yield and found that the temperature at the point of infection had the greatest influence on bacteriophage yield for both bioprocesses. The study also aimed to develop a scaled down shake‐flask model to enable rapid optimization of bacteriophage batch bioprocessing and translate the bioprocess into a scale‐up model with a 3 L working volume in stirred tank bioreactors. The optimization performed in the shake flask model achieved a 550‐fold increase in bacteriophage yield and these improvements successfully translated to the large‐scale cultures.

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Section: Studies Have Shown That Approximately 54% Of Predominantmentioning

confidence: 99%

A scaled‐down model for the translation of bacteriophage culture to manufacturing scale

Ali

Rafiq

Ratcliffe

2019

Biotech & Bioengineering

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“…Untersuchungen mittels Prozesstomographie bzw. lokal vorgenommene Messungen der Strömungsgeschwindigkeit haben gezeigt, dass sich unter Ansatz gleicher Rührerumfangsgeschwindigkeiten in geometrisch ähnlich ausgebildeten Reaktoren vergleichbare Strömungsgeschwindigkeiten ausbilden . Daher wurden die Rührer bei den Umfangsgeschwindigkeiten von 5,5 und 6,7 m s −1 betrieben.…”

Section: Experimenteller Aufbauunclassified

Investigation of Hydrodynamics in Biogas Digesters with Oval Geometry by Instrumented Flow Followers

Reinecke

Jobst

Hampel

2017

Chemie Ingenieur Technik

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Instrumented flow following sensor particles with magnetic position detection have been used to study the hydrodynamics of model biogas digesters with oval geometry in laboratory and pilot plant scale. Vertical residence profiles and circulation time distributions are extracted from the sensor particle data to characterize the macroscopic flow in the digesters. In particular, the oval digesters are compared regarding mixing homogeneity and suspension of particles

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“…1). Mathematisch lässt sich das Verhältnis der Drehzahlen im Original (o) und im Modell (m) über den Skalierungsmaßstab sowie das Verhältnis der jeweiligen Dichten ρ beschreiben , .

true \frac{n_{normalm}}{n_{normalo}} = \frac{d_{normalo}}{d_{normalm}} \sqrt{\frac{ρ_{normalo}}{ρ_{normalm}}}

…”

Section: Materials Und Methodenunclassified

“…Allerdings wurde die Auswirkung partikelfreier und partikelbeladener Fluide bei gleichem Viskositätsverhalten der Suspensionen auf das Strömungsfeld nicht betrachtet. Dies ist nach wie vor Stand der Forschung . Die verwendete Messtechnik erlaubt darüber hinaus nur eine großskalige, approximierte Bestimmung von Geschwindigkeitskomponenten und eignet sich für die detaillierte Charakterisierung des Strömungsfeldes nur bedingt.…”

Section: Introductionunclassified

Einfluss der Rührwerksposition auf den Mischprozess in Biogasanlagen anhand eines Paddelrührwerks

Annas

Elfering

Volbert

et al. 2019

Chemie Ingenieur Technik

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Die Auslegung und Optimierung von Rührsystemen in Biogasanlagen erfolgt nach wie vor auf Basis empirischer Erfahrungswerte. Mithilfe der particle image velocimetry (PIV) wurde das Strömungsfeld eines Paddelrührwerks unter dem Einfluss der Einbaulage und der Fluidviskosität im Labormaßstab untersucht. Dabei wurden die Viskositätseigenschaften von Fermentersuspensionen mithilfe eines Rohrviskosimeters bestimmt. Über Maßstabsgesetze konnten die Viskositätseigenschaften der Modellsuspensionen für den Versuch angepasst werden. Als Ergebnis zeigt sich, dass eine geänderte Einbaulage Totzonen um bis zu 55 % reduzieren kann.

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Scale-up of mixing processes of highly concentrated suspensions using electrical resistance tomography

Cited by 20 publications

References 6 publications

A scaled‐down model for the translation of bacteriophage culture to manufacturing scale

A scaled‐down model for the translation of bacteriophage culture to manufacturing scale

Investigation of Hydrodynamics in Biogas Digesters with Oval Geometry by Instrumented Flow Followers

Einfluss der Rührwerksposition auf den Mischprozess in Biogasanlagen anhand eines Paddelrührwerks

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