Scalable Parallel Manipulation of Single Cells Using Micronozzle Array Integrated with Bidirectional Electrokinetic Pumps

Nagai, Masao; Kato, Keita; Soga, Satoshi; Santra, Tuhin Subhra; Shibata, Takayuki

doi:10.3390/mi11040442

Cited by 7 publications

(4 citation statements)

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“…Furthermore, single-cell bioprinting with the inkjet printing method could be achieved only by diluting the cell suspension to reduce the cell density [80,82,83]. Therefore, to increase the reliability and efficiency of single-cell printing, various new techniques have been developed to improve the dispensing efficiency of inkjet-like single-cell printing [40][41][42][44][45][46]68,[84][85][86][87][88][89][90][91][92][93][94][95][96][97][98][99].…”

Section: Noncontact Printingmentioning

confidence: 99%

“…Second, the printed single cells or colonies can be easily recovered with addressability for subsequent analysis. Third, it is convenient to integrate the highly efficient single-cell printing with other techniques, such as imaging system [40,41], electric field [42][43][44], and acoustic field [45,46], and the single-cell encapsulation efficiency can reach more than 90%. By comparison, the theoretical limit of the single-cell capture efficiency of the widely used droplet-based microfluidic approach is only 37% according to Poisson's distribution.…”

Section: Introductionmentioning

confidence: 99%

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Advances in Single-Cell Printing

Zhou¹,

Wu²,

Wen³

et al. 2022

Micromachines

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Single-cell analysis is becoming an indispensable tool in modern biological and medical research. Single-cell isolation is the key step for single-cell analysis. Single-cell printing shows several distinct advantages among the single-cell isolation techniques, such as precise deposition, high encapsulation efficiency, and easy recovery. Therefore, recent developments in single-cell printing have attracted extensive attention. We review herein the recently developed bioprinting strategies with single-cell resolution, with a special focus on inkjet-like single-cell printing. First, we discuss the common cell printing strategies and introduce several typical and advanced printing strategies. Then, we introduce several typical applications based on single-cell printing, from single-cell array screening and mass spectrometry-based single-cell analysis to three-dimensional tissue formation. In the last part, we discuss the pros and cons of the single-cell strategies and provide a brief outlook for single-cell printing.

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Section: Noncontact Printingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advances in Single-Cell Printing

Zhou¹,

Wu²,

Wen³

et al. 2022

Micromachines

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“…Miniaturization of space components has promoted the development of micro/nano-thrusters in the space propulsion system, where the electrokinetic propulsion technology becomes a new solution of space-related tasks [ 3 , 4 , 5 , 6 , 7 , 8 ]. Motivated by the electrokinetic electroosmosis principle, electroosmosis actuation with an external electric field is extensively applied to micro/nano-fluidics and electric machines, such as micropump devices and small thrusters, which are associated with the electric double layer (EDL) in micro/nanochannels [ 9 , 10 , 11 ]. The EDL is generated due to the interaction of ionized solution with static charges on dielectric surfaces.…”

Section: Introductionmentioning

confidence: 99%

Space Electroosmotic Thrusters in Ion Partitioning Soft Nanochannels

Zheng

Jian

2021

Micromachines

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Space electroosmotic thrusters (EOTs) are theoretically investigated in a soft charged nanochannel with a dense polyelectrolyte layer (PEL), which is considered to be more realistic than a low-density PEL. When the PEL is dense, its permittivity is smaller than the one of the electrolyte solution layer, leading to rearrangement of ions in the channel, which is denoted as the ion partitioning effect. It is noted that fluid viscosity becomes high within the PEL owing to the hydration effect. An analytical solution for electroosmotic velocity through the channel is obtained by utilizing the Debye–Hückel linearization assumption. Based on the fluid motion, thruster performances, including thrust, specific impulse, thrust-to-power ratio, and efficiency, are calculated. The ion partitioning effect leads to enhancement of the thruster velocity, while increase of the dynamic viscosity inside the PEL reduces the flow rate of the fluid. Therefore, these performances are further impacted by the dense soft material, which are discussed in detail. Moreover, changes or improvements of the thruster performances from the dense PEL to the weak PEL are presented and compared, and distributions of various energy items are also provided in this study. There is a good result whereby the increase in electric double layer thickness promotes the development of thruster performances. Ultimately, the simulated EOTs produce thrust of about 0 to 20 μN and achieve thruster efficiency of 90.40%, while maintaining an appropriate thrust–power ratio of about 1.53 mN/W by optimizing all design parameters.

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“…The high-throughput in vivo cellular microenvironment can permit us to investigate cellular function in detail. Nagai et al [ 29 ] developed a parallel single-cell manipulation using a micronozzle array compacted with a bidirectional electrokinetic micropump. The polydimethylsiloxane (PDMS) micro nozzle array combined with bidirectional electrokinetic pumps are operated by using DC-biased AC voltages.…”

mentioning

confidence: 99%