Scalable Manufacturing of Human Mesenchymal Stromal Cells in the Vertical‐Wheel Bioreactor System: An Experimental and Economic Approach

A Scalable System for Generation of Mesenchymal Stem Cells Derived from Induced Pluripotent Cells Employing Bioreactors and Degradable Microcarriers

“…When the ihMSCs were seeded at 1000 and 6600 cells per cm 2 (a seeding density similar to those used in other studies 12 ) and incubated for a further 7 days under the same conditions, both GelMA microcarrier formulations supported cell expansion to approximately 12 000 cells per cm 2 with no sign of cytotoxicity (Figure 2B). GelMA microcarriers are theoretically digestible, dismissing the need for separation.…”

Turning Nature’s own processes into design strategies for living bone implant biomanufacturing: a decade of Developmental Engineering

“…Bioreactor systems allowing efficient expansion of progenitor cells should be used and evidence of this has already been provided for example in multi-stack and hollow-fiber bioreactors [ [125] , [126] , [127] ]. There exist several types of suspension culture using microcarriers in stirred bioreactors [ 128 , 129 ] such as vertical wheel systems [ 130 , 131 ], wave bags [ 132 ] and mostly small scale stirred systems (typically spinner flasks of 100-200 ml) [ [133] , [134] , [135] ]. Studies showing that BM-MSCs can be expanded in 5L stirred tanks [ 136 ] and 50L Mobius® bioreactors demonstrate that large scale production of single cell progenitor cells is feasible and could provide adequate quantities of cells for further differentiation.…”

Engineering microenvironments for manufacturing therapeutic cells

“…47 Combined analysis of cell yields and bioprocess economic modeling of microcarrier systems have also shown that microcarrier systems in a vertical wheel bioreactor can reduce the total manufacturing costs per dose by nearly a half relative to standard tissue culture plastic expansion. 48 The combined analysis of manufacturing costs and yield was shown to be particularly valuable in comparing the manufacturing of MSCs in a multi-layer vessel, stirred tank bioreactor with microcarriers, a hollow fiber bioreactor, and a packed bed bioreactor; while MSCs had the greatest fold expansion in the hollow fiber bioreactor, multi-layer vessels were shown to be the most cost-effective in terms of yield. 49…”