SAYP and Brahma are important for ‘repressive’ and ‘transient’ Pol II pausing

Vorobyeva, Nadezhda E.; Nikolenko, J. V.; Набирочкина, Е. Н.; Краснов, А. Н.; Shidlovskii, Yulii V.; Георгиева, С. Г.

doi:10.1093/nar/gks472

Cited by 34 publications

(26 citation statements)

References 42 publications

(71 reference statements)

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“…In our previous studies we have described in details the scheme which makes possible to activate the Drosophila developmental ftz-f1 gene in S2 Schneider cells [13]. A simultaneous recruitment of the BAP170 and OSA subunits to the promoter of this gene in a non-activated stage (in normal S2 Schneider cells) was demonstrated above.…”

Section: Pbap But Not Bap Subcomplex Of the Dswi/snf Complex Is Accummentioning

confidence: 84%

“…According to our previous studies, the promoter of the ftz-f1 ecdysone cascade gene with a high affinity binds the PBAP subcomplex of the dSWI/SNF [13]. It was demonstrated for the PB-and SAYP-specific subunits of PBAP subcomplex.…”

Section: Both Pbap and Bap Subcomplexes Of Dswi/snf Chromatin Remodelmentioning

confidence: 94%

“…Moreover dSWI/SNF has been revealed to be important for the transient pausing of RNA Polymerase II during active transcription. So, the significance of the dSWI/SNF for the proper elongation and Ser2 CTD phosphorylation marker loading has been demonstrated for the same gene during the active transcription state [13]. Furthermore, the influence of the SWI/SNF complex on the RNA Polymerase II transition to the elongation state has been reported for the human.…”

Section: Introductionmentioning

confidence: 89%

“…The locus of rDNA was chosen as a negative control because in our previous studies it did not bind the common subunits of the dSWI/SNF complex [13]. The results of chromatin immunoprecipitation are shown on Figure 3.…”

Section: Both Pbap and Bap Subcomplexes Of Dswi/snf Chromatin Remodelmentioning

confidence: 99%

“…To evaluate which one of the dSWI/SNF subcomplexes assists RNA Polymerase II in elongation process we have studied the re-distribution of the subunits on the ftz-f1 gene upon transcription activation. Earlier, we have described the multistep scheme of the ftz-f1 gene activation by the addition and subsequent withdrawal of the 20 hydroxyecdysone (below, referred to simply as ecdysone) hormone into the S2 Schneider cells culturing medium [13]. The main steps of the induction system are schematically presented in the Figure 4.…”

Section: Pbap But Not Bap Subcomplex Of the Dswi/snf Complex Is Accummentioning

confidence: 99%

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SWI/SNF Chromatin Remodeling Complex Involved in RNA Polymerase II Elongation Process in Drosophila melanogaster

Vorobyeva¹,

Mazina²,

Doronin³

2013

Chromatin Remodelling

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Section: Pbap But Not Bap Subcomplex Of the Dswi/snf Complex Is Accummentioning

confidence: 84%

Section: Both Pbap and Bap Subcomplexes Of Dswi/snf Chromatin Remodelmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 89%

Section: Both Pbap and Bap Subcomplexes Of Dswi/snf Chromatin Remodelmentioning

confidence: 99%

Section: Pbap But Not Bap Subcomplex Of the Dswi/snf Complex Is Accummentioning

confidence: 99%

See 3 more Smart Citations

SWI/SNF Chromatin Remodeling Complex Involved in RNA Polymerase II Elongation Process in Drosophila melanogaster

Vorobyeva¹,

Mazina²,

Doronin³

2013

Chromatin Remodelling

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The SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA

et al. 2022

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BRG1 and BRM are ATPase core subunits of the human SWI/SNF chromatin remodelling complexes mainly associated with transcriptional initiation. They also have a role in alternative splicing, which has been shown for BRM-containing SWI/SNF complexes at a few genes. Here, we have identified a subset of genes which harbour alternative exons that are affected by SWI/SNF ATPases by expressing the ATPases BRG1 and BRM in C33A cells, a BRG1- and BRM-deficient cell line, and analysed the effect on splicing by RNA sequencing. BRG1- and BRM-affected sub-sets of genes favouring both exon inclusion and exon skipping, with only a minor overlap between the ATPase. Some of the changes in alternative splicing induced by BRG1 and BRM expression did not require the ATPase activity. The BRG1-ATPase independent included exons displayed an exon signature of a high GC content. By investigating three genes with exons affected by the BRG-ATPase-deficient variant, we show that these exons accumulated phosphorylated RNA pol II CTD, both serine 2 and serine 5 phosphorylation, without an enrichment of the RNA polymerase II. The ATPases were recruited to the alternative exons, together with both core and signature subunits of SWI/SNF complexes, and promoted the binding of RNA binding factors to chromatin and RNA at the alternative exons. The interaction with the nascent RNP, however, did not reflect the association to chromatin. The hnRNPL, hnRNPU and SAM68 proteins associated with chromatin in cells expressing BRG1 and BRM wild type, but the binding of hnRNPU to the nascent RNP was excluded. This suggests that SWI/SNF can regulate alternative splicing by interacting with splicing-RNA binding factor and influence their binding to the nascent pre-mRNA particle.

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