Saponins in Tumor Therapy

Bachran, Christopher; Bachran, Silke; Sutherland, Mark; Bachran, Diana; Fuchs, Hendrik

doi:10.2174/138955708784534445

Cited by 90 publications

(70 citation statements)

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“…4;2013 Soyasaponins I, III and soyasapogenol B decreased cell proliferation in human colon cancer cells in a time and concentration-dependent manner (Figures 2-4). Previous studies (Bachran et al, 2008;Berhow et al, 2000;Ellington et al, 2005Ellington et al, , 2006Koratkar & Rao, 1997) utilizing different cell lines , different soyasaponin mixtures, and concentrations, demonstrated less cell growth inhibition than the current study. When reaching the 48 and 72 hour time points in the previously listed studies, cell growth curves of soyasaponin mixtures were still progressing steadily upwards, but in the present study, cells growth plateaus after 24 hours or begin to die off after 48 hours eventually returning to near 0 hour cell numbers after 72 hours.…”

Section: Discussioncontrasting

confidence: 61%

Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Salyer¹,

Eswaranandam²,

Lee³

2013

JFR

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Group B saponins, the predominant form of saponins in heat-treated soy products, have been shown to possess hypocholesterolemic, antimutagenic, and anticarcinogenic properties. Previous studies have evaluated crude mixtures of soyasaponins, but studies evaluating a single purified soyasaponin as an anticarcinogenic agent are limited. The goal of this study is to examine the effects of purified soyasaponins I and III as well as their aglycone form, soyasapogenol B, as anticarcinogenic agents on the human colon adenocarcinoma cell line (Caco-2). Experiments were conducted to examine the effects of purified soyasaponins on cell proliferation, Protein Kinase C (PKC) activity, and cell morphology in Caco-2 cell cultures. Treatment of cells with soyasaponins I and III at concentrations of 0.3-0.9 mg/ml significantly reduced viable cell numbers after 48 and 72 hours of exposure by 8.6-65.3% (p < 0.05). Soyasapogenol B at a concentration of 0.15 mg/ml significantly reduced viable cell numbers after 24 hours by 62.4% (p < 0.05). Cell morphology changes demonstrated that as concentrations and lipophilicity of soyasaponins increased, cell membranes became rougher and more irregular. Treatment of cells for 72 hours significantly reduced the amount of PKC activity by 9-40% (p < 0.05). Results indicate that purified soyasaponins I, III and soyasapogenol B can suppress Caco-2 colon cancer cell proliferation. These findings suggest that purified group B soyasaponins and their final metabolite soyasapogenol B may be a colon-cancer suppressive component of soy that warrants further examination as a potential nutraceutical or functional food.

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Section: Discussioncontrasting

confidence: 61%

Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Salyer¹,

Eswaranandam²,

Lee³

2013

JFR

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“…(Khan et al, 2006), having structural similarity with saikosaponins (Hsu et al, 2000;Mahato & Pal, 1987). Saikosaponins and saikosaponin-like compounds have been reported to possess potent antitumor activity (Bachran et al, 2008). In a study of Mallick et al (2010), methanol extract of Corchorus acutangulus, its n-butanol fraction, and corchorusin-D inhibited cell growth and produced significant cytotoxicity in leukemic cell lines U937 and HL-60 via mitochondrial apoptotic pathway.…”

Section: Discussionmentioning

confidence: 99%

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

İşeri

Yurtçu²,

Şahin

et al. 2013

Pharmaceutical Biology

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Context: Corchorus olitorius L. (Malvaceae) has industrial importance in world jute production and is a widely cultivated and consumed crop in Cyprus and in some Arabic countries. Objective: The present study investigated cytotoxic and genotoxic effects of leaf extracts (LE) and seed extracts (SE) of the C. olitorius on the multiple myeloma-derived ARH-77 cells. The extracts were also evaluated for their total phenol content (TPC) and free radical scavenging activity (FRSA). Materials and methods: C. olitorius was collected from Nicosia, Cyprus. TPC and FRSA were measured by Folin-Ciocalteu and DPPH free radical methods, respectively. Cytotoxicity was evaluated by the MTT assay (4-2048 mg/mL range), and DNA damage (at IC 50 and ½IC 50 ) was measured by the comet assay. Results and discussion: The LE had significantly higher total phenol (78 mg GAE/g extract) than the SE (2 mg GAE/g extract) with significantly higher FRSA (IC 50 LE: 23 mg/mL and IC 50 SE: 10 401 mg/mL). Both LE and SE exerted cytotoxic effects on cells after 48 h. The IC 50 of SE (17 mg/mL) was lower than LE (151 mg/mL), which demonstrates its higher cytotoxicity on cells. The extracts were applied at 150 and 75 mg/mL for LE and at 17 and 8.5 mg/mL for SE, and the results of the comet assay revealed that the extracts induced genotoxic damage on ARH-77 cells. In both 48 h leaf and seed extract treatments, genotoxic damage significantly increased with increasing concentrations at relevant cytotoxic concentrations. Conclusion: To our knowledge, this is the first report demonstrating the high cytotoxic potential of C. olitorius SE and the genotoxic potential of LE and SE.

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“…Saponins have been ascribed a number of pharmacological actions, 1,14-16 the important ones being permeabilizing of the cell membrane, 4 lowering of serum cholesterol levels, 17 stimulation of luteinizing hormone release leading to abortifacient properties, 17 immunomodulatory potential via cytokine interplay, 14 cytostatic and cytotoxic effects on malignant tumor cells, 18 adjuvant properties for vaccines as immunostimulatory complexes, 19 and synergistic enhancement of the toxicity of immunotoxins. 20,21 A number of other activities have been attributed to saponins, and the literature is replete with various pharmacological properties ascribed to the saponins in general.…”

Section: Pharmacological Studiesmentioning

confidence: 99%

Chemistry and pharmacology of saponins: special focus on cytotoxic properties

Thakur¹,

Melzig²,

Fuchs³

et al. 2011

BTAT

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Saponins are bioactive compounds produced mainly by plants but also by some marine organisms and insects. In the recent past, there has been unforeseen interest in the clinical utilization of saponins as chemotherapeutic agents. The research on saponins in various forms as a treatment for cancer has generated a lot of potential. The advent of nanotechnology and the cytotoxicity enhancing properties of saponins are some of the highlights of the current decade. This review gives an updated overview of the clinical potential that saponins hold as cytotoxic agents, and covers the literature for 1957-2011, with the main focus on research conducted in the last decade. It is conceivable that saponins hold a lot of therapeutic potential and could be a lead for identification of synthetic or semisynthetic molecules for the treatment of cancer via membrane-mediated or transport-mediated pathways.

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Saponins in Tumor Therapy

Cited by 90 publications

References 0 publications

Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Soyasaponin I, III, and Soyasapogenol B Inhibit Proliferation and Modulate PKC Expression in Caco-2 Human Colon Cancer Cells

Corchorus olitorius(jute) extract induced cytotoxicity and genotoxicity on human multiple myeloma cells (ARH-77)

Chemistry and pharmacology of saponins: special focus on cytotoxic properties

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