Sand flies and Leishmania: specific versus permissive vectors

Volf, Petr; Myšková, Jitka

doi:10.1016/j.pt.2006.12.010

Cited by 130 publications

(104 citation statements)

References 12 publications

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“…The cause of the low host specificity in Leishmania species in lizards is unknown, but we speculate that it may stem from low host specificity of the vectors. As stressed by Volf and Peckova (2007), the classification of sandfly species into specific or permissive vectors is likely to be an over simplification of the true complexity of parasite-sandfly interactions. Perhaps due to the modes of transmission of these parasites, Leishmania species in lizards have a significantly lower degree of host specificity.…”

Section: Discussionmentioning

confidence: 99%

Molecular detection, identification and phylogenetic inference of Leishmania spp. in some desert lizards from Northwest China by using internal transcribed spacer 1 (ITS1) sequences

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Molecular detection, identification and phylogenetic inference of Leishmania spp. in some desert lizards from Northwest China by using internal transcribed spacer 1 (ITS1) sequences

et al. 2016

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“…3A). Neither extending the incubation time nor increasing the concentration of UTP significantly altered the profile obtained 2 . Importantly, neither full-length nor small products were produced by corresponding preparations from LRV1-negative parasites (Fig.…”

Section: Resultsmentioning

confidence: 77%

“…The neglected tropical disease leishmaniasis is caused by species of the genus Leishmania, which are single-celled eukaryotic parasites transmitted by phlebotomine sand flies (1,2). Leishmaniasis occurs in many regions of the world, with more than 12 million cases and more than 1.7 billion people at risk (1,3).…”

Section: Introductionmentioning

confidence: 99%

Concentration of 2′C-methyladenosine triphosphate by Leishmania guyanensis enables specific inhibition of Leishmania RNA virus 1 via its RNA polymerase

Robinson

Beverley

2018

Journal of Biological Chemistry

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Leishmania is a widespread trypanosomatid protozoan parasite causing significant morbidity and mortality in humans. The endobiont dsRNA virus Leishmania RNA virus 1 (LRV1) chronically infects some strains, where it increases parasite numbers and virulence in murine leishmaniasis models, and correlates with increased treatment failure in human disease. Previously, we reported that 2'-C-methyladenosine (2CMA) potently inhibited LRV1 in Leishmania guyanensis (Lgy) and L. braziliensis, leading to viral eradication at concentrations above 10 µM. Here we probed the cellular mechanisms of 2CMA inhibition, involving metabolism, accumulation and inhibition of the viral RNA dependent RNA polymerase (RDRP) . Activation to 2CMA triphosphate (2CMA-TP) was required, as 2CMA showed no inhibition of RDRP activity from virions purified on cesium chloride gradients. In contrast, 2CMA-TP showed IC50s ranging from 150 to 910 µM, depending on the CsCl density of the virion (empty, ssRNA-and dsRNA-containing). Lgy parasites incubated in vitro with 10 µM 2CMA accumulated 2CMA-TP to 410 µM, greater than the most sensitive RDRP IC50 measured. Quantitative modeling showed good agreement between the degree of LRV1 RDRP inhibition and LRV1 levels. These results establish that 2CMA activity is due to its conversion to 2CMA-TP, which accumulates to levels that inhibit RDRP and cause LRV1 loss. This attests to the impact of the Leishmania purine uptake and metabolism pathways, which allow even a weak RDRP inhibitor to effectively eradicate LRV1 at micromolar concentrations. Future RDRP inhibitors with increased potency may have potential therapeutic applications for ameliorating the increased Leishmania pathogenicity conferred by LRV1.

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“…Algunos vectores son específicos para una sola especie de patógeno (Ej. Phlebotomus papatasi solo transmite L. major en el Viejo Mundo) (26) , mientras que los vectores permisivos son los que tienen capacidad de transmitir diferentes especies del mismo patógeno (Ej. Lu.…”

unclassified

“…Además de la detección del ADN de Leishmania en el potencial vector por PCR, el reciente hallazgo de receptores de lipofosfoglicanos (LPG) de Leishmania en el tracto digestivo de algunas especies de flebotomíneos por técnicas bioquímicas y moleculares es un importante aporte en la determinación de su rol vectorial, pues se sabe que la unión de Leishmania a estos receptores LPG es crucial para evitar la acción de las enzimas digestivas y permitir el desarrollo del parásito en el vector (6,26) .…”

unclassified

Vectores de la leishmaniasis tegumentaria y la Enfermedad de Carrión en el Perú: una actualización

Zorrilla

Vásquez

Espada

et al. 2017

Rev Peru Med Exp Salud Publica

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Sand flies and Leishmania: specific versus permissive vectors

Cited by 130 publications

References 12 publications

Molecular detection, identification and phylogenetic inference of Leishmania spp. in some desert lizards from Northwest China by using internal transcribed spacer 1 (ITS1) sequences

Molecular detection, identification and phylogenetic inference of Leishmania spp. in some desert lizards from Northwest China by using internal transcribed spacer 1 (ITS1) sequences

Concentration of 2′C-methyladenosine triphosphate by Leishmania guyanensis enables specific inhibition of Leishmania RNA virus 1 via its RNA polymerase

Vectores de la leishmaniasis tegumentaria y la Enfermedad de Carrión en el Perú: una actualización

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