Sampling oscilloscope as a coherent wide-band detector

Bletzinger, P.; Garscadden, A.; Alexeff, I.; Jones, W. D.

doi:10.1088/0950-7671/42/5/424

Cited by 7 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When the sampling frequency is insufficient to resolve the time course of a signal of interest, one way to improve time resolution is to perform repetitive measurements in a way in which the data are only sampled once after the trigger with a variable time interval between trigger and sampling, which can be much shorter than the sampling interval. This method was originally applied using the sampling oscilloscope (14). In cellular imaging experiments, a 350-ns pulsed laser was used to generate snapshots sampling fura-2 fluorescence images at various times after different individual stimuli during the exposure of a cooled CCD camera (15).…”

Section: Discussionmentioning

confidence: 99%

Precise Time Superresolution by Event Correlation Microscopy

Fang

Zhao

Lindau

2019

Biophysical Journal

View full text Add to dashboard Cite

Fluorescence imaging is often used to monitor dynamic cellular functions under conditions of very low light intensities to avoid photodamage to the cell and rapid photobleaching. Determination of the time of a fluorescence change relative to a rapid high time-resolution event, such as an action potential or pulse stimulation, is challenged by the low photon rate and the need to use imaging frame durations that limit the time resolution. To overcome these limitations, we developed a time superresolution method named event correlation microscopy that aligns repetitive events with respect to the high time-resolution events. We describe the algorithm of the method, its step response function, and a theoretical, computational, and experimental analysis of its precision, providing guidelines for camera exposure time settings depending on imaging signal properties and camera parameters for optimal time resolution. We also demonstrate the utility of the method to recover rapid nonstepwise kinetics by deconvolution fits. The event correlation microscopy method provides time superresolution beyond the photon rate limit and imaging frame duration with well-defined precision.

show abstract

Section: Discussionmentioning

confidence: 99%