RETRACTED ARTICLE: Salt in stools is associated with obesity, gut halophilic microbiota and Akkermansia muciniphila depletion in humans

Seck, El hadji; Senghor, Bruno; Merhej, Vicky; Bachar, Dipankar; Cadoret, F.; Robert, C.; Azhar, Esam I.; Yasir, Muhammad; Bibi, Fehmida; Jiman-Fatani, Asif A.; Konaté, Drissa; Musso, Didier; Doumbo, Ogobara K.; Sokhna, C.; Levasseur, Anthony; Lagier, Jean-Christophe; Khelaifia, Saber; Million, Matthieu; Raoult, Didier

doi:10.1038/s41366-018-0201-3

Cited by 54 publications

(45 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A positive correlation between the richness of the halophilic bacteria and faecal salinity has been recently reported [19]. To verify this association, we randomly selected 20 faecal samples with different per cent relative abundances of haloarchaea, i.e.…”

Section: Diversity Of Haloarchaeal Phylotypes In the Human Gutmentioning

confidence: 79%

“…For instance, our previous study was the rst to report the presence of haloarchaea in faecal samples of Korean subjects in 2008 based on a conventional molecular ecology method [18]. More recently, viable haloarchaeal strains (belonging to the genus Haloferax) were isolated from human faeces [19,20], and two genome sequences of the human gut-derived haloarchaeal strains (i.e. Haloferax massiliensis and Halorubrum lipolyticum) are currently available [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

Kim¹,

Whon²,

Lim

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Archaea are one of the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although limited information regarding the diversity and abundance of other archaeal phylotypes is available.Results: We surveyed the archaeome of faecal samples collected from 897 East Asian subjects living in South Korea. In total, 42.47% faecal samples were positive for archaeal colonisation; these were subsequently subjected to archaeal 16S rRNA gene deep sequencing and real-time quantitative polymerase chain reaction-based abundance estimation. The mean archaeal relative abundance was 10.24% ± 4.58% of the total bacterial and archaeal abundance. We observed extensive colonisation of haloarchaea (95.54%) in the archaea-positive faecal samples, with 9.63% mean relative abundance in archaeal communities. Haloarchaea were relatively more abundant than methanogens in some samples. The presence of haloarchaea was also verified by fluorescence in situ hybridisation analysis. Owing to large inter-individual variations, we categorised the human gut archaeome into four archaeal enterotypes.Conclusions: The study demonstrated that the human gut archaeome is indigenous, responsive, and functional, expanding our understanding of the archaeal signature in the gut of human individuals.

show abstract

Section: Diversity Of Haloarchaeal Phylotypes In the Human Gutmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 99%

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

Kim¹,

Whon²,

Lim

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…A positive correlation between the richness of the halophilic bacteria and faecal salinity has been recently reported [18]. To verify this association, we randomly selected 20 faecal samples with different proportional abundances of haloarchaea, i.e., 5.38 -99.33% relative abundances, and measured faecal salinity using a salinity refractometer, yielding a mean salinity of 0.68%, ranging from 0.30-1.05% (see Additional file 1: Supplementary Fig.…”

Section: Diversity Of Haloarchaeal Phylotypes In the Human Gutmentioning

confidence: 78%

“…Haloarchaea is increasingly detected in habitats of relatively low salinities [41,42], supporting the possible growth of haloarchaea in the human gut. Higher faecal salinity might not necessarily promote haloarchaeal growth in the human gut, as evidenced by the absence of haloarchaea-assigned sequence, in which the mean salinity is 0.7% and ranging from 0% to 6% [18]. Members of haloarchaea can grow either aerobically or anaerobically.…”

Section: Pcr Amplicon Sequencing Analysis Revealed That Sequences Assmentioning

confidence: 99%

“…For instance, we were the first to report the presence of haloarchaea in faecal samples of Korean subjects in 2008 based on a conventional molecular ecology method [17]. More recently, Seck et al isolated viable haloarchaeal strains (belonging to the genus Haloferax) from human faeces [18]. However, the presence of the haloarchaeal community and their collective genomes (hereafter termed 'haloarchaeome') in the human gut were not confirmed despite the use of metataxonomic analysis, i.e., the archaeal 16S rRNA gene-targeted amplicon sequencing.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

Kim

Whon

Lim

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Archaea are the least-studied members of the gut-dwelling autochthonous microbiota. Few studies have reported the exclusive dominance of methanogens in the archaeal microbiome (archaeome) of the human gut, although information regarding the diversity and abundance of other archaeal phylotypes is limited. Results We surveyed the archaeome in the faecal samples collected from 897 normal East Asian subjects living in South Korea. In total, 42.47% faecal samples were positive for archaeal colonization, which were subsequently subjected to archaeal 16S rRNA gene deep sequencing and abundance estimation. The mean archaeal abundance was 9.89 ± 4.48% of the total bacterial and archaeal abundance. We observed extensive colonization of haloarchaea (95.53%) in the Korean gut, with 9.63% mean relative abundance in archaeal communities. Haloarchaea were relatively more abundant than methanogens in certain samples. The presence of haloarchaea was also verified by fluorescence in situ hybridization analysis. Owing to large inter-individual variations, we categorized the human gut archaeome into four archaeal enterotypes. Conclusions The study demonstrated that the human gut archaeome is indigenous, responsive, and functional, expanding our understanding of the archaeal signature in the gut of normal healthy individuals.

show abstract

Meta-analyses on the Periodontal Archaeome

et al. 2022

View full text Add to dashboard Cite

RETRACTED ARTICLE: Salt in stools is associated with obesity, gut halophilic microbiota and Akkermansia muciniphila depletion in humans

Cited by 54 publications

References 46 publications

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

The human gut archaeome: identification of diverse haloarchaea in Korean subjects

Meta-analyses on the Periodontal Archaeome

Contact Info

Product

Resources

About