Salmonella spp investigation in transport boxes of day-old birds

The objective of this research was to isolate and to verify the sensitivity to antimicrobial agents of strains of Salmonella sp. isolated from poultry products in the state of Ceara, Brazil. A total number of 114 samples was collected from 63 broiler carcasses derived from two processing plants and two supermarkets, and 51 excreta samples were collected in broiler farms located in the state of Ceara, which used three live production stages. Each excreta sample consisted of a fresh excreta pool from 100 birds. Samples were submitted to microbiological analyses, and the isolated Salmonella strains were tested for antimicrobial sensitivity. No Salmonella was isolated from excreta samples, while broiler carcass samples showed a high contamination rate of11.8%. Three serotypes were identified: Salmonella enterica serovar Enteritidis, 50%; Salmonella enterica serovar Panama 33%, and Salmonella enterica serovar Newport, 17%. As to the susceptibility tests to antimicrobial agents, 100% of the isolated Salmonella strains showed resistance to Ampicillin and Tetracycline, and sensitivity to Gentamycin, Netilmycin, Carbenicillin, Chloramphenicol.

Section: Discussionmentioning

confidence: 99%

“…Salmonella is one of the most important sources of food-borne illness in humans, mainly due to the complexity of Salmonellosis epidemiology (Zancan et al 2000). Broiler meat is a high risk product, according to , and production systems and processing facilitate the presence of Salmonella in the final product.…”

Section: Discussionmentioning

confidence: 99%

Initial identification and sensitivity to antimicrobial agents of Salmonella sp.isolated from poultry products in the state of Ceara, Brazil

Oliveira

Cardoso

Salles

et al. 2006

“…in order to ensure that they were free from this pathogen. The procedure was performed according to Zancan et al (2000), with modifications, as follows: individual cloacal swabs impregnated with selenite-cystine and novobiocin (40µg/mL) (SCNov) were directly streaked onto brilliant green agar plates containing nalidixic acid (100µg/mL) (VBNal) and incubated at37ºC for 24h. Negative samples for Salmonella spp.…”

Section: Bacteriological Pre-inoculation Monitoringmentioning

confidence: 99%

Recovery of Salmonella Gallinarum in the Organs of Experimentally-Inoculated Japanese Quails (Coturnix coturnix)

Silva

Cardoso²,

Teixeira³

et al. 2015

Salmonellosis is an infection caused by specific or non specific serotypes of the Salmonella genus, responsible for losses in the poultry industry. Fowl typhoid, caused by S. Gallinarum (SG) is important because it causes elevated mortality in adult birds, leading to economic losses in the poultry industry. This study aimed at quantifying the number of viable SG cells in the liver, spleen, lung, cecum, and reproductive tract (ovary and testicles) of experimentally inoculated Japanese quails (Coturnix coturnix), as well as SG shedding in their feces. One hundred and two Japanese quails, with four months of age at the beginning of the experiment, were used. The birds were inoculated with three bacterial cultures containing different concentrations (6x10 4 CFU/0.1mL, 2x105 CFU/0.4mL, or 5x10 6 CFU/0.2mL) of SG resistant to nalidixic acid. On days 1, 4, 7, and 14 after the inoculation (dpi) individual cloacal swabs were collected from six birds per group, which were subsequently sacrificed for organ sampling. The swab samples were streaked directly on plates containing brilliant green agar and nalidixic acid (VBNal). Samples that were negative after 24h, were streaked again. The collected organs were individually macerated and transferred to buffered peptone water at 0.1%. The solutions were immediately diluted serially for CFU counting in VBNal. SG was successfully recovered from one quail, which was inoculated with 2x10 5 CFU/0.4mL, and from five quails of the group inoculated with 5x10 6 CFU/0.2mL inoculum. All of the analyzed cloacal swab samples were negative. Therefore, this study demonstrated it was difficult to isolate SG from the analyzed organs and that it was not possible to recover thepathogen in the cloacal swabs collected from inoculated quails. These results may be explained by the absence of flagella in SG, inducing weak intestinal immune response in the beginning of the infection and preventing its isolation in cloacal swab samples. The low positivity rate of the analyzed organs may be due to the immune status of the euthanized birds, since the SG dissemination in the animal organism occurs mostly close to death, which was observed in the birds found dead during the experiment.

“…Birds were analyzed according to methodology of Zancan et al (2000) with modifications. Fresh feces and cloacal swabs were collected per cage (pool of three birds) using sterile material.…”

Section: Bacteriological Monitoring Before Inoculationmentioning

confidence: 99%

Experimental infection of Salmonella Enteritidis in quails submitted to forced molting by feed fasting

Santos

Teixeira²,

Lopes³

et al. 2015

This study aimed at evaluating bacterial shedding, as detected in swabs, feces, and eggs of quails submitted to forced molting by feed fasting and experimentally infected with a Salmonella Enteritidis (SE) strain. In the experiment, 84 40-week-old Italian female quails were distributed in the following groups: FI (quails induced to molt by fasting and inoculated with Salmonella Enteritidis -SE); CI (quails fed with a laying diet and inoculated with SE); FNI (quails induced to molt by fasting and not inoculated with SE); and CNI (quails fed with a laying feed and not inoculated with SE). Feces, cloacal swabs, and eggs were collected on day 1, 3, 7 and 14 post-inoculation (dpi) and submitted to bacteriological analyses. All samples obtained from cloacal swabs were negative for SE. None of the quails of the non-inoculated groups (FNI and CNI) were positive for SE in the fecal samples. Among the inoculated quails, the FI group presented significantly higher (p< 0.05) SE shedding in the feces on 1 dpi than the CI group. On 4 dpi, no significant difference was observed (p< 0.05) in SE shedding between the inoculated quail groups. On 7 dpi, only the FI group shed SE in the feces, whereas on 14 dpi, none of the groups shed SE. According to the results, we concluded that quails submitted to molting by fasting have higher possibility of shedding SE in the feces.