Salmonella serovars along two beef chains in Ethiopia

Hiko, Adem; Irsigler, Herlinde; Ameni, Gobena; Zessin, Karl-Hans; Fries, R.

doi:10.3855/jidc.6354

Cited by 13 publications

(14 citation statements)

References 12 publications

(46 reference statements)

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“…The proportion of Salmonella isolates, in the current study, recovered from swabs of carcasses of abattoir origin was 14.29%. This finding corroborates with the findings of Takele et al 6 and Dabassa and Bacha 30 who reported 11.3% and 13.3% from beef carcasses in Jimma municipal abattoir, respectively, and Hiko et al 24 who reported 11.8% from Addis Ababa Abattoirs Enterprise whereas this prevalence is lower than other reports from Tigray region (16.4%), 31 Dire Dawa abattoir (17.7%), 32 and Senegal (42.8%). 33 Counter to this, the current finding is higher than the report of El-Gamal and EL-Bahi, 34 Renatus et al, 35 Thongsay et al, 36 Kalambhe et al, 37 Bahnass et al, 38 Sefinew and Bayleyegn, 39 and Gizachew and Mulugeta 40 from Egypt (0.0%), Namibia (0.50%), Thailand (4.5%), Central India (6%), Saudi Arabia (8.5%), and Bahir Dar (4.8, 7.6%), respectively.…”

Section: Discussionsupporting

confidence: 89%

“…The current finding is higher than the report of Shilangale et al, 21 who reported a 0.85% incidence of Salmonella from the three beef export abattoirs in Namibia and the 1.5% isolation rate reported by Ibrahim 22 from cattle carcasses at Kano abattoir in Nigeria. Contrarily, this finding is lower than the reports of Wassie et al, 23 Hiko et al, 24 and Amenu 25 from Gonder (20.8%), Addis Ababa (26.6%), and Arbaminch (31.5%) municipal abattoirs, respectively.…”

Section: Discussioncontrasting

confidence: 54%

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Carriage, Risk Factors, and Antimicrobial Resistance Patterns of Salmonella Isolates from Raw Beef in Jimma, Southwestern Ethiopia

Geresu

Desta

2021

IDR

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Purpose Consumption of raw beef infected with multidrug-resistant Salmonella is pertinent to the world public health risk of antimicrobial resistance. Henceforth, this study aimed to investigate the carriage, antimicrobial resistance (AR) patterns, and the revealing risk factors of Salmonella-contaminating beef in abattoirs and butcher shops in Jimma town. Methods A cross-sectional study was conducted to investigate the carriage and AR patterns and to reveal the risk factors of beef contaminated by Salmonella spp. Three hundred and forty-eight swab samples were collected from abattoirs (n=210) and butcher shops (n=138) and the conventional cultural methods were employed for identification of Salmonella. Isolates were subjected to 12 antimicrobials using the Kirby–Bauer disk diffusion method for AR patterns. Results The occurrence of Salmonella isolates from the abattoir samples was 11.4%, whereas about 6.52% of isolates were recovered from butcher shops. Educational status (abattoir: odds ratio (OR)=8.40, confidence interval (CI)=1.186–59.493; butcher shops: OR=9.17, CI=1.15–73.239), job related training (abattoir: OR=5.50, CI=1.065–28.416), contamination risk perception (abattoir: OR=5.31, CI=1.256–22.489), neatness of knives (abattoir: OR=7.6, CI=0.892–65.376), source of contamination (abattoir: OR=8.44, CI=1.682–42.39), wearing of protective cloth (butcher shops: OR=8.44, CI=1.682–42.39), manner of hand washing (butcher shops: OR=7.25, CI=1.210–43.442), and money handling (butcher shops: OR= 9.69, CI=1.578–59.474) were among the potential risk factors significantly associated with Salmonella carcass contamination in the abattoir and butcher shops. Of the 33 Salmonella isolates, 14 (58.3%) and six (66.7%) of the abattoir and butcher shops isolates, correspondingly, were resistant to two or more antibiotics. Conclusion The finding of this investigation exhibited extensive multidrug-resistant Salmonella isolates in the study setting. Hence, establishing standard meat safety requirements and provision of training for meat handlers and prudent use of antimicrobials are recommended.

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Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 54%

Carriage, Risk Factors, and Antimicrobial Resistance Patterns of Salmonella Isolates from Raw Beef in Jimma, Southwestern Ethiopia

Geresu

Desta

2021

IDR

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“…Salmonella Saintpaul was previously reported from camel (Molla, Salah, Alemayehu, & Mohammed, ) and minced beef (Zewdu & Cornelius, ) in the country. Another recent study also showed that S. Saintpaul was the dominant serovar in beef abattoir and beef processing plant in Addis Ababa(Hiko, Irsigler, Ameni, Zessin, & Fries, ).…”

Section: Discussionmentioning

confidence: 92%

“…Salmonella Saintpaul was previously reported from camel (Molla, Salah, Alemayehu, & Mohammed, 2004) and minced beef (Zewdu & Cornelius, 2009) in the country. Another recent study also showed that S. Saintpaul was the dominant serovar in beef abattoir and beef processing plant in Addis Ababa (Hiko, Irsigler, Ameni, Zessin, & Fries, 2016 Salmonella carried by cockroaches in a neonatal intensive care unit (Tilahun et al, 2012), and another study also showed high mortality from blood stream infection in TASH due to MDR enterobacteriaceae (Seboxa et al, 2015).…”

Section: Discussionmentioning

confidence: 94%

Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia

Eguale

Asrat

Alemayehu

et al. 2018

Zoonoses and Public Health

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Salmonella is one of the common causes of food-borne bacterial illnesses. The primary sources of human nontyphoidal Salmonella (NTS) infection are food animals. This study characterized temporally and spatially related Salmonella isolated during April 2013 to March 2014 from faeces of diarrhoeic human patients in Addis Ababa (n = 68) and food animals (n = 84) in Addis Ababa and surrounding districts (dairy cattle, n = 30; slaughtered cattle, n = 20; poultry, n = 26; swine n = 8). Isolates were serotyped, page typed and tested for antimicrobial susceptibility using Kirby-Bauer disc diffusion method, and genotyped by pulsed-field gel electrophoresis (PFGE). The dominant Salmonella serovars isolated from food animals were S. Saintpaul (38.1%), S. Typhimurium (17.9%) and S. Kentucky (9.5%), whereas in humans, S. Typhimurium (39.7%), S. Virchow (30.9%) and S. Kottbus (10.3%) were frequently isolated. Resistance to streptomycin, sulfisoxazole, tetracycline, ampicillin and cephalothin was higher in animal isolates than human isolates, and mean number of antimicrobials to which isolates were resistant was significantly higher in isolates from cattle and poultry compared to those from humans (p < 0.05). All S. Kentucky isolated from animals and humans were multidrug resistant (MDR) with shared resistance phenotype (AmpCfCipTeSuSNa). Although this study involved small sample size and was not able to show clear epidemiological linkage among isolates from various sources, genotyping by PFGE analysis demonstrated circulation of closely related genotypes of S. Virchow, S. Typhimurium and S. Kentucky among humans and food animals. Detection of related Salmonella isolates from humans and animals, the high MDR status of isolates from animals and close proximity of farms and human residential areas in the absence of appropriate biosecurity present major public health problem. Integrated surveillance of Salmonella serovars in humans and animals and implementation of appropriate hazard analysis and pathogen control strategies along critical points of the food chain from farm to table is recommended.

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“…In Ethiopia, different Salmonella serovars were isolated from animals and humans (Alemayehu et al, 2003;Tibaijuka et al;Ejeta et al, 2004;Aragaw et al, 2007;Zewdu and Cornelius, 2009;Sibhat et al, 2011;Beyene et al, 2011;Hiko et al, 2016;Kiflu et al, 2017;Hiko et al, 2018). However, PFGE analysis of the isolates was not conducted; thus establishing genetic relationships for epidemiological purposes is impossible.…”

Section: Introductionmentioning

confidence: 99%

PFGE XbaI® Indistinguishable properties of Salmonella Kastrup and Salmonella Larochelle isolates at beef processing and distribution continuum

Hiko¹,

Agga²,

Bräutigam³

et al. 2019

Ethiop. Vet. J.

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Salmonellosis is a major cause of foodborne illnesses in humans throughout the world. Pulsed field gel electrophoresis (PFGE) is a commonly used tool for epidemiological survey and source tracking of Salmonella strains isolated from various sources. A total of 237 samples from various sources were collected at abattoir and butcheries in Ethiopia from December 2011 to April 2012. Isolates of Salmonella Kastrup (n = 3) and Salmonella Larochelle (n = 11) were characterized for antimicrobial resistance using disc diffusion and for genotypic profiles using PFGE XbaI ®. Salmonella Kastrup and S. Larochelle were isolated from 1.3% and 4.6% of total samples (n = 237) examined. Except for water samples (n = 12) and truck swabs (n=11), all other sample sources were positive for one or more of S. Kastrup and S. Larochelle. Salmonella Kastrup was isolated in Ethiopia for the first time. Salmonella isolates from both Kastrup and Larochelle serotypes showed similar antimicrobial resistance profiles. Two pulsotypes consisting of the same three S. Kastrup and 10 S. Larochelle cluster and second cluster consisting of one S. Larochelle were identified at 1.5% and 1% band positions tolerance by unweighted-pair group method (UPGM) and showed ≥91% similarity. S. Kastrup and S. Larochelle isolates showed similar antimicrobial resistance profiles with indistinguishable PFGE XbaI ® patterns indicating possible clonal spread of the isolates along beef processing and distribution continuum.

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Salmonella serovars along two beef chains in Ethiopia

Cited by 13 publications

References 12 publications

Carriage, Risk Factors, and Antimicrobial Resistance Patterns of Salmonella Isolates from Raw Beef in Jimma, Southwestern Ethiopia

Carriage, Risk Factors, and Antimicrobial Resistance Patterns of Salmonella Isolates from Raw Beef in Jimma, Southwestern Ethiopia

Phenotypic and genotypic characterization of temporally related nontyphoidal Salmonella strains isolated from humans and food animals in central Ethiopia

PFGE XbaI® Indistinguishable properties of Salmonella Kastrup and Salmonella Larochelle isolates at beef processing and distribution continuum

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