Salmonella Enteritidis in Shell Eggs

Yousef, Ahmed E.

doi:10.1016/b978-0-12-394382-8.00007-1

Cited by 6 publications

(2 citation statements)

References 108 publications

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“…Serotype Enteritidis was chosen as a non-host strain for multiple reasons: (i) ser. Enteritidis is a common serotype found in cases of gastroenteritis [66,67]; (ii) it is fully resistant to χ; (iii) it only produces a single flagellin without undergoing phase variation; and (iv) its H antigen type is g,m, with g antigen flagellins having been shown to be χ resistant by Meynell [50] and confirmed by our data (S1 Table ). It is worth noting that despite being fully resistant to χ, this serotype allows a very small level of adsorption similar to the Flastrain (Fig 4).…”

Section: Strain Selection and Elimination Of Phase Variation And Meth...supporting

confidence: 80%

Phages on filaments: A genetic screen elucidates the complex interactions between Salmonella enterica flagellin and bacteriophage Chi

Esteves,

Bigham,

Scharf

2023

PLoS Pathog

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The bacterial flagellum is a rotary motor organelle and important virulence factor that propels motile pathogenic bacteria, such as Salmonella enterica, through their surroundings. Bacteriophages, or phages, are viruses that solely infect bacteria. As such, phages have myriad applications in the healthcare field, including phage therapy against antibiotic-resistant bacterial pathogens. Bacteriophage χ (Chi) is a flagellum-dependent (flagellotropic) bacteriophage, which begins its infection cycle by attaching its long tail fiber to the S. enterica flagellar filament as its primary receptor. The interactions between phage and flagellum are poorly understood, as are the reasons that χ only kills certain Salmonella serotypes while others entirely evade phage infection. In this study, we used molecular cloning, targeted mutagenesis, heterologous flagellin expression, and phage-host interaction assays to determine which domains within the flagellar filament protein flagellin mediate this complex interaction. We identified the antigenic N- and C-terminal D2 domains as essential for phage χ binding, with the hypervariable central D3 domain playing a less crucial role. Here, we report that the primary structure of the Salmonella flagellin D2 domains is the major determinant of χ adhesion. The phage susceptibility of a strain is directly tied to these domains. We additionally uncovered important information about flagellar function. The central and most variable domain, D3, is not required for motility in S. Typhimurium 14028s, as it can be deleted or its sequence composition can be significantly altered with minimal impacts on motility. Further knowledge about the complex interactions between flagellotropic phage χ and its primary bacterial receptor may allow genetic engineering of its host range for use as targeted antimicrobial therapy against motile pathogens of the χ-host genera Salmonella, Escherichia, or Serratia.

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Section: Strain Selection and Elimination Of Phase Variation And Meth...supporting

confidence: 80%

Phages on filaments: A genetic screen elucidates the complex interactions between Salmonella enterica flagellin and bacteriophage Chi

Esteves,

Bigham,

Scharf

2023

PLoS Pathog

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“…Salmonella enteritidis (SE) is a foodborne pathogen hosted by both humans and various animals, which could cause the death of livestock, resulting in serious economic losses [ 1 , 2 ] and seriously endangering human health by contaminating livestock products [ 3 ]. Consequently, the creation of quick and precise detection techniques is crucial for the management and prevention of SE disease [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of Salmonella enteritidis in Penaeus vannamei

Ling

et al. 2023

Molecules

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Salmonella enteritidis (SE) is an important factor causing foodborne disease, and electrochemical sensors have drawn much attention for SE prevention and detection due to their many advantages. A renewable electrochemical sensor using specially designed locked nucleic acids (LNA) as linkers for the detection of SE was proposed to improve the reusability and reproducibility of biosensors. One end of the LNA was designed as an anchor to attach to modified electrodes through the sulfhydryl group; the other end was used to match with a short segment of SE aptamers, which will allow for the convenient renewal of occupied aptamers by raising the temperature. Results revealed that the manufactured biosensor had good stability, reproducibility, and selectivity in addition to a linear range of 6 × 101–6 × 105 CFU/mL and a limit of detection (LOD) of 20.704 CFU/mL. The recovery rate of SE for the real sample varied from 98.84% to 134.82% without exceeding 16.27% in the relative standard deviation (RSD). The proposed biosensor appears to be a promising tool for foodborne pathogen detection.

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Diversification, Innovation and Safety of Local Cuisines and Processed Food Products

Kuang

Bhat

2017

Sustainability Challenges in the Agrofood Sector

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Salmonella Enteritidis in Shell Eggs

Cited by 6 publications

References 108 publications

Phages on filaments: A genetic screen elucidates the complex interactions between Salmonella enterica flagellin and bacteriophage Chi

Phages on filaments: A genetic screen elucidates the complex interactions between Salmonella enterica flagellin and bacteriophage Chi

A Renewable Biosensor Based on LNA-Aptamer Hybridization for the Detection of Salmonella enteritidis in Penaeus vannamei

Diversification, Innovation and Safety of Local Cuisines and Processed Food Products

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